Fig. 3 | Nature Communications

Fig. 3

From: Anti-LRP5/6 VHHs promote differentiation of Wnt-hypersensitive intestinal stem cells

Fig. 3

Structural analysis of L-P2-B10 and L-P2-D07 anti-LRP6 binding mode. a Alignment of the LRP6P3E3P4E4 complexes with L-P2-B10 and LRP6–L-P2-D07 in two orthogonal views. Cartoon trace with transparent surface representation of LRP6P3E3 (blue), LRP6P4E4 (green), L-P2-B10 (orange) and L-P2-D07 (red). b Interface of LRP6–L-P2-B10 with on the left-hand side LRP6P3 (gray) and CDR2 and CDR3 loops of L-P2-B10 (green and red, respectively). Interacting side chains are shown as sticks. On the right side, surface presentation of L-P2-B10 (orange) with interacting residues of LRP6P3 shown in sticks; numbers represent the six blades of β-propeller 3. c Interface of LRP6–L-P2-D07 complex, presented similar to b, with in addition the CDR1 loop in blue (left) and the surface of L-P2-D07 in red (right). d Binding-surface areas of L-P2-B10 (orange), L-P2-D07 (red) and DKK-C31 (magenta) on LRP6P3E3P4E4 (gray)

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