Fig. 2 | Nature Communications

Fig. 2

From: Anti-LRP5/6 VHHs promote differentiation of Wnt-hypersensitive intestinal stem cells

Fig. 2

Anti-LRP6P3E3P4E4 VHHs show nM affinities. a, b Luciferase activities normalized to unstimulated control are represented as a heat map. Anti-LRP6P3E3P4E4 VHHs cause a dose-dependent decrease of Wnt signaling in both HEK293T cells overexpressing LRP6 (a) or untransfected HEK293T cells (b). c Binding affinities of anti-LRP6P3E3P4E4 VHHs as revealed by ITC measurements. One representative experimental titration curve of each VHH binding to LRP6P3E3P4E4 is shown. DP: differential power, kD: dissociation constant, N: stoichiometry, ΔH: delta enthalpy, −TΔS: temperature delta entropy

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