Fig. 1 | Nature Communications

Fig. 1

From: Anti-LRP5/6 VHHs promote differentiation of Wnt-hypersensitive intestinal stem cells

Fig. 1

VHHs targeting LRP6P3E3P4E4 block cellular responses to Wnt3a. a Schematic representation of LRP6. The P3E3P4E4 module of the extracellular domain was used to generate anti-LRP6 VHHs. Coloring scheme: LRP6P1E1; yellow/orange, LRP6P2E2; pink/orange, LRP6P3E3; blue/orange and LRP6P4E4; green/orange. LA domains are shown in brown. b Wnt luciferase reporter assay performed in LRP6-overexpressing HEK293T cells stimulated with Wnt3a-conditioned medium and treated with 10 μM of the indicated anti-LRP6P3E3P4E4 VHHs. c Wnt luciferase reporter assay performed in HEK293T cells stimulated with Wnt3a-conditioned medium and treated with 10 μM of the indicated anti-LRP6P3E3P4E4 VHHs. Graphs show average (bars) and range (dots) of luciferase activity in duplicate cell cultures transfected in parallel

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