Fig. 3 | Nature Communications

Fig. 3

From: Interferon lambda protects the female reproductive tract against Zika virus infection

Fig. 3

Hormone treatment modulates the severity of ZIKV infection in the vagina of OVX Ifnlr1−/− and hSTAT2 KI Ifnlr1−/− mice. Six-week-old WT, Ifnlr1−/−, hSTAT2 KI, or hSTAT2 KI Ifnlr1−/− OVX mice were given a hormone replacement regimen of estradiol, progesterone, estradiol and progesterone, or vehicle. WT and Ifnlr1−/− mice were treated with 1 mg of anti-Ifnar1 mAb at day −1. On day 0, mice were inoculated via intravaginal route with 106 FFU of ZIKV (Dakar 41525). At 3 dpi, vaginal lavage was performed (b). At 7 dpi, ZIKV RNA was measured from the vagina (a, e), cervix (c, f), and uterus (d, g). Dotted lines indicate the LOD. Results are pooled from two or three experiments. Bars indicate median values (Mann–Whitney test: (*, P < 0.05 **, P < 0.01). Numbers of mice: Vehicle: WT + anti-Ifnar1, n = 10; Ifnlr1−/− + anti-Ifnar1, n = 7. Progesterone: WT + anti-Ifnar1, n = 16; Ifnlr1−/− + anti-Ifnar1, n = 11; hSTAT2 KI, n = 7; or hSTAT2 KI Ifnlr1−/−, n = 5. Estradiol: WT + anti-Ifnar1, n = 10; Ifnlr1−/− + anti-Ifnar1, n = 6. Estradiol and progesterone: WT + anti-Ifnar1, n = 9; Ifnlr1−/− + anti-Ifnar1, n = 13

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