Aggressive behaviours of solid tumours are highly influenced by the tumour microenvironment. Multiple signalling pathways can affect the normal function of stromal fibroblasts in tumours, but how these events are coordinated to generate tumour-promoting cancer-associated fibroblasts (CAFs) is not well understood. Here we show that stromal expression of Dickkopf-3 (DKK3) is associated with aggressive breast, colorectal and ovarian cancers. We demonstrate that DKK3 is a HSF1 effector that modulates the pro-tumorigenic behaviour of CAFs in vitro and in vivo. DKK3 orchestrates a concomitant activation of β-catenin and YAP/TAZ. Whereas β-catenin is dispensable for CAF-mediated ECM remodelling, cancer cell growth and invasion, DKK3-driven YAP/TAZ activation is required to induce tumour-promoting phenotypes. Mechanistically, DKK3 in CAFs acts via canonical Wnt signalling by interfering with the negative regulator Kremen and increasing cell-surface levels of LRP6. This work reveals an unpredicted link between HSF1, Wnt signalling and YAP/TAZ relevant for the generation of tumour-promoting CAFs.
Stepwise acquisition of genetic alterations is crucial for the initiation of primary epithelial tumours. Yet, increasing evidence supports the notion that concomitant stromal changes play a critical role in cancer progression in many types of neoplasias1,2. Fibroblasts constitute a significant proportion of the stromal compartment in many solid tumours. As opposed to normal fibroblasts, which are generally anti-tumorigenic3, cancer-associated fibroblasts (CAFs) present a pathological activated phenotype that enables them to influence tumour progression, dissemination and response to therapy through remodelling of the extracellular matrix (ECM) and signalling to cancer, endothelial and immune cells4,5. In CAFs, signalling pathways such as Heat-Shock Factor 1 (HSF1) and YAP/TAZ are activated in response to cellular stress and mechanical cues, respectively6,7. In turn, HSF1 affects signalling to cancer cells promoting tumour growth whereas YAP promotes cancer cell invasion and angiogenesis through remodelling of the ECM. Thus, each pathway is regulated by different mechanisms and controls a defined set of functions; whether these molecular events are interconnected to regulate the emergence of a fully activated CAF phenotype is not known.
Dickkopf (DKK) proteins comprise a conserved family of secreted negative regulators of β-catenin8. DKK1, DKK2 and DKK4 have been shown to antagonise Wnt-mediated β-catenin stabilisation by binding and down-modulating Wnt co-receptor LRP5/69,10. In contrast, DKK3 does not interact with LRP5/6 and thus is not considered a true Wnt signalling antagonist. The role of DKK proteins in cancer is thought to be mainly tumour suppressive, as they are commonly downregulated in cancer cells and can negatively affect proliferation and survival. Yet, the role of DKK proteins in cancer stroma is still understudied.
In this study we uncover an unprecedented role for DKK3 in linking HSF1 and YAP/TAZ signalling. We demonstrate that DKK3 is a HSF1 target gene that promotes aggressive behaviours in CAFs by potentiating YAP/TAZ activity via canonical Wnt signalling. Mechanistically, we show that DKK3 promotes LRP5/6 activity by interfering with the negative Wnt regulator Kremen1/2.
DKK3 expression levels in the tumour microenvironment
The initial evidence suggesting that DKK3 may play a role in the regulation of the tumour microenvironment came from analyses of stromal gene expression in normal and cancerous tissues (Supplementary Figure 1a). DKK3 gene and protein expression is significantly upregulated in the tumour stroma in several types of cancers including breast, colon and ovarian (Supplementary Figure 1a and Fig. 1a–c). Stromal expression of other DKK genes across various cancer types was less consistent (Supplementary Figure 1a), suggesting that DKK3 is the only DKK factor commonly associated with cancer stroma. DKK3 protein levels in breast cancer (BC) stroma were significantly increased upon progression to more aggressive cancers, particularly in ER-negative BC (Fig. 1d and Supplementary Figure 1b). Furthermore, in ER-negative BC there was a significant association between high stromal DKK3 gene expression and poor outcome (Fig. 1e). Analysis of human BC tissues showed that stromal DKK3 expression was restricted to vimentin-positive cells (Fig. 1f), an expression pattern characteristic of CAFs11,12. To investigate the cell of origin of DKK3 expression, we isolated different cell populations of murine MMTV-PyMT mammary carcinomas (Supplementary Figure 1c&d, see Methods for details). Dkk3 expression was restricted to CAFs (Fig. 1g). Similar findings were obtained in a human setting13 (Supplementary Figure 1e). Stromal DKK3 expression in human tumours positively correlated with the expression of CAF markers ACTA2, FAP and COL1A2 (Fig. 1h), supporting a link between DKK3 and CAFs. As DKK3 was restricted to CAFs in tumours, we investigated the prognostic potential of whole tumour DKK3 expression. These analyses indicated a significant correlation between DKK3 gene expression and poor outcome in ER-negative BC patients, as well as in colon and ovarian cancer patients (Fig. 1i).
DKK3 is a HSF1 target gene upregulated in CAFs
To investigate DKK3 in tractable models, we isolated CAFs from murine mammary carcinomas and human breast, colon and ovarian cancers, as well as normal tissue counterparts (NFs). We observed consistent upregulation of DKK3 mRNA and protein levels in CAFs compared to NFs and cancer cells (Fig. 2a, b and Supplementary Figure 2a&b). Comprehensive analysis of these models indicated that DKK3 expression was stronger in FAP-positive CAFs (Supplementary Figure 2b-d) but not necessarily associated with DKK3 secretion (Fig. 2b). Noteworthy, the DKK gene DKK2 also showed a significant enrichment in the cancerous stroma of some tumour types (Supplementary Figure 1a). However, DKK2 expression was not restricted to CAFs in murine or human tumours (Fig. 1g and Supplementary Figure 1d&e), nor upregulated in murine or human CAFs when compared to NFs (Fig. 2a, b and Supplementary Figure 2b). In addition, no association between DKK2 expression and poor outcome was observed in ER-negative BC, colon and ovarian cancer patients (Fig. 1i). For these reasons, we decided to focus on studying the role of DKK3 in CAFs.
To investigate the mechanism leading to DKK3 upregulation in CAFs, we first analysed the ChEA Transcription Factor Binding Site dataset in the Enrich database14,15. This informed of 58 transcription factors that potentially bind the DKK3 promoter (Fig. 2c). Of these, HSF1 was the only factor to positively modulate DKK3 expression in perturbation assays16. This was further confirmed in two independent datasets involving Hsf1 deletion in fibroblasts6,17 (Supplementary Figure 2e). HSF1 is a transcription factor that can be activated by stresses such as oxidative stress, nutrient-deprivation and protein misfolding18, which are commonly found in the tumour microenvironment. HSF1 has been associated to aggressive CAF behaviours by its ability to drive a transcriptional programme that supports the malignant potential of cancer in a non-cell-autonomous way6. Silencing Hsf1 expression reduced Dkk3 mRNA and protein expression in CAFs (Fig. 2f, g). Furthermore, HSF1 activity strongly correlated with DKK3 gene expression in the stromal compartment of human tumours (Fig. 2h). Next, we performed chromatin immunoprecipitation using anti-HSF1 antibodies and extracts from murine NF and CAFs. Fig. 2i shows that Hsf1 was significantly more associated with the bona fide HSF1 target gene Rilpl in CAFs. Further, Hsf1 interacted with promoter and enhancer regions of the Dkk3 locus, and this association was significantly increased in CAFs.
DKK3 modulates pro-tumorigenic functions in CAFs
To investigate the functional relevance of DKK3 we silenced Dkk3 expression in murine CAF1 with 2 independent RNAi (Fig. 3a). Knocking-down Dkk3 did not affect the expression of genes associated to CAF phenotypes (Fig. 3b). However, the ability of CAFs to contract collagen gels, a measure of their matrix remodelling capacity, was dependent on Dkk3 expression (Fig. 3c). Decreased gel contraction in Dkk3-siRNA CAFs was associated with thinner collagen fibres (Fig. 3d) and a significant decrease in matrix stiffness (Fig. 3e). Similar findings were obtained in CAF5 (Supplementary Figure 3a), a CAF line that did not secrete Dkk3 (Fig. 2b). For long-term functional analyses, we generated Dkk3 knockout CAFs (CAF-KO) and CAF-KO re-expressing Dkk3 (CAF-KO-REC)(Supplementary Figure 3b and Fig. 3f). Validating our previous results, Dkk3-null CAFs presented significantly less ECM remodelling activity (Supplementary Figure 3c, d). In three-dimensional (3D) co-culture models19 (Fig. 3g), CAFs promoted cancer cell invasion and growth of murine MMTV-PyMT TS1 cancer cells, and this ability was dependent on Dkk3 expression (Fig. 3h, i). These results were further validated using alternative approaches (i.e. matrigel on top) (Supplementary Figure 3e&f) and in human patient-derived CAFs from breast, colorectal and ovarian cancers (Supplementary Figure 4). Conversely, forcing DKK3 expression in NFs (Supplementary Figure 5a) significantly increased their abilities to remodel collagen matrices and promote TS1 invasion (Supplementary Figure 5b&c). In CAFs, HSF1 promotes tumour growth by inducing the production of secreted factors such as TGFβ and SDF16. Using recombinant DKK3 and CAF-derived conditioned media from WT, KO and KO-REC CAFs we observed that secreted DKK3 had no effect on cancer cell proliferation and motility (Supplementary Figure 5d). Validating these results, we observed no significant differences in cancer cell proliferation or motility between cells treated with conditioned media from CAF1 (detectable secreted DKK3, as per Fig. 2b) or CAF5 (no detectable secreted DKK3), or in the presence of a blocking antibody against DKK3 (Supplementary Figure 5e). In addition, secreted DKK3 was not able to recover the functional defects associated to loss of DKK3 in CAFs (Supplementary Figure 5f). Thus, DKK3 is a novel HSF1 target gene that regulates ECM remodelling and associated cancer cell growth and invasion; unlike other HSF1 target genes such as TGFβ or SDF1, DKK3 does not have any apparent secreted function.
DKK3 is required for the tumour-promoting activities of CAFs in vivo
In vivo (Fig. 4a), we observed that tumours arising from cancer cells co-injected with KO-CAFs grew significantly more slowly than those of mice co-injected with WT-CAFs or KO-REC-CAFs, leading to increased survival (Fig. 4b, c). Conversely, ectopic expression of DKK3 in NF significantly increased their ability to promote tumour growth in vivo (Supplementary Figure 5g). Histological analysis revealed that TS1 + WT-CAF or TS1 + KO-REC-CAF tumours shared a poorly differentiated morphology and invasive phenotypes typical of high-grade tumours, with loss of basal lamina (i.e. laminin staining), despite having similar number of αSMA-positive CAFs (Fig. 5d). In contrast, TS1 + KO-CAFs tumours had a more differentiated architecture where cancer cell acini where surrounded by laminin-rich areas. Fibronectin and Masson’s trichrome staining revealed increased ECM deposition at the tumour border in TS1 + WT-CAF and TS1 + KO-REC-CAF tumours, suggesting a higher reactive stroma. Second-harmonic generation (SHG) imaging confirmed these findings as TS1 + WT-CAFs tumours presented a higher content of collagen fibres than TS1 + KO-CAF tumours (Fig. 5f). These ECM characteristics have been linked with aggressive CAF phenotypes as they promote cancer cell motility and local invasion20,21. In agreement, we observed areas at the tumour border where cancer cells were invading in TS1 + WT-CAFs tumours (Fig. 5f, asterisk). Using intravital imaging we confirmed this as co-injection of TS1 cells and WT-CAFs produced tumours where both cancer cells and CAFs were highly motile (Supplementary Movie 1; Fig. 5g, h). In contrast, motility in tumours with KO-CAFs was severely impaired (Supplementary Movie 2; Fig. 5h).
DKK3 in CAFs potentiates Wnt/β-catenin and YAP/TAZ signalling
To understand the molecular mechanism whereby DKK3 exerts its functions and how HSF1 connects to other signalling pathways, we performed global transcriptomic profiling of murine CAFs after Dkk3 silencing. Gene set enrichment analysis22 revealed a significant decrease in the expression of genes associated with Wnt/β-catenin and YAP/TAZ activities (Fig. 5a, Supplementary Figure 6a and Supplementary Table 1). β-catenin and YAP/TAZ are transcriptional regulators with paramount roles in development and cancer23,24. In CAFs, YAP establishes a transcriptional programme that enhances matrix remodelling and invasion of neighbouring cancer cells7. β-catenin plays a pivotal role in fibrotic disorders25, but its relevance in modulating CAF functions is still not well understood. Dkk3 knock-down decreased levels of non-phosphorylated (active) β-catenin and TAZ levels (Fig. 5b), reduced nuclear YAP/TAZ localisation (Fig. 5c) and inhibited β-catenin and YAP/TAZ transcriptional activity (Fig. 5d). These findings were confirmed using Dkk3 knockout/recovery system (Supplementary Figure 6b&c) and patient-derived CAFs (Supplementary Figure 6d-f). Conversely, ectopic expression of DKK3 in human NFs increased non-phosphorylated (active) β-catenin and YAP/TAZ levels (Supplementary Figure 6g). Furthermore, we observed reduced β-catenin and YAP staining in DKK3-null CAFs in vivo (Fig. 5e, f), and DKK3 expression significantly correlated with YAP/TAZ and β-catenin signatures in human cancer stroma (Fig. 5g). We confirmed that β-catenin and YAP/TAZ were activated in murine and human CAFs (Fig. 5h and Supplementary Figure 7a&b), and in the stroma of breast, colorectal and ovarian cancers (Supplementary Figure 7c).
DKK3 regulates CAF functions via YAP/TAZ
Using our battery of functional assays we tested whether DKK3 was regulating CAF functions via YAP/TAZ and/or β-catenin. Contrary to YAP/TAZ silencing, knocking-down β-catenin (Fig. 6a) had no effect in the ability of CAFs to contract collagen-rich matrices (Fig. 6b), or promote cancer cell invasion and growth in 3D (Fig. 6c, d). Loss of YAP/TAZ function in CAFs leads to MLC2 and Src inactivation and reduced actomyosin contractility, which affects the ability of CAFs to remodel the ECM7. Dkk3 knockdown was associated with reduced phospho-MLC2 and phospho-Src levels (Supplementary Figure 7d). β-catenin siRNA treatment did not affect MLC2 or Src activation (Fig. 6a), suggesting that DKK3 loss-of-function is primarily associated to its effect on YAP/TAZ activity. Confirming this, expression of a constitutively active mutant of YAP in Dkk3-null CAFs was able to recover their gel remodelling and cancer cell growth promoting abilities (Fig. 6e, f). Both DKK3 and YAP gain-of-function phenotypes were associated with reactivation of actomyosin contractility and Src (Fig. 6g). Overall, these data supported a model where HSF1 in CAFs leads to DKK3 upregulation, which in turn potentiates YAP/TAZ activity leading to increased ECM remodelling and promotion of cancer cell growth and invasion (Fig. 6h). In agreement, we observed that silencing Hsf1 in CAFs led to β-catenin and YAP/TAZ inactivation (Fig. 6i and Supplementary Figure 7e) and was associated with a reduction in the matrix remodelling activity of CAFs (Fig. 6j). However, these defects were prevented by constitutive expression of DKK3 or YAPS5A.
DKK3 potentiates canonical Wnt signalling in CAFs
We next investigated the molecular mechanism whereby DKK3 modulates YAP/TAZ. We observed that the expression levels of key regulators of YAP/TAZ function, MST1/2 and LATS kinases26, was marginally affected in one of the Dkk3-null clones (i.e. KO.9) when compared to wild-type and KO-REC CAFs (Supplementary Figure 8a). However, analysis of YAP S127 phosphorylation, the main maker of Hippo activity over YAP26,27,28, showed no differences after DKK3 modulation. This suggested that DKK3 might be acting independently of Hippo signalling. To confirm this, we silenced LATS1&2 expression in wild type and KO.9 CAFs and assessed CAF functions (Supplementary Figure 8b). Silencing LATS1&2 increased the abilities of CAFs to remodel gels and promote cancer cell growth (Supplementary Figure 8c, d), in line with their negative role in YAP function. However, the presence or absence of DKK3 did not significantly alter the effect of inhibiting Hippo signalling in CAF activities or YAP/TAZ levels, excluding a role for Hippo kinases in YAP/TAZ activation through DKK3.
Recent studies have shown that, similar to β-catenin, YAP/TAZ stability and function are positively modulated in response to canonical Wnt signalling29,30. Thus, association of LRP5/6 co-receptors with Axin1 in response to Wnt stimulation displaces β-catenin and YAP/TAZ from the destruction complex. In the absence of LRP5/6 signal, Axin1 targets β-catenin and YAP/TAZ for degradation. Gene expression analyses indicated that modulating DKK3 expression levels did not alter the expression of Wnt signalling regulators (Supplementary Figure 8e). However, our previous data suggested that DKK3 depletion/ectopic expression was affecting β-catenin and YAP/TAZ protein expression (Supplementary Figures 6b&g and Supplementary Figure 8a). To confirm whether DKK3 was affecting the activity of the destruction complex, we performed cycloheximide-chase assays. Fig. 7a shows that the protein stability of β-catenin and YAP/TAZ was severely affected in the absence of DKK3. Further analyses revealed that LRP6 levels at the cell surface were diminished when Dkk3 was knocked-out (Fig. 7b), suggesting that DKK3 was affecting YAP/TAZ activity and CAF functions at the level of LRP5/6. In agreement, targeting Lrp5/6 expression in WT-CAFs (Supplementary Figure 8f), attenuated β-catenin and YAP activation (Fig. 7c and Supplementary Figure 8f) and diminished their tumour-promoting activities (Fig. 7d, e). Yet, silencing Lrp5/6 expression in Dkk3-null CAFs had no functional consequences. Inhibiting canonical Wnt signalling with XAV93931 yielded similar results (Supplementary Figure 7g&h), suggesting that this was a canonical Wnt-related function. To further confirm this, we investigated the role of DKK3 in CAFs after Wnt ligand stimulation. We observed that stimulation of wild type and Dkk3-null CAFs with canonical Wnt ligand Wnt3a induced a significant increase in their basal (i.e. low serum) gel remodelling activities, whereas non-canonical Wnt5a stimulation had no effect (Fig. 7f). Even though DKK3 was not absolutely required for CAFs to respond to Wnt3a, the gel remodelling activities of CAFs after Wnt3a stimulation were significantly reduced in the absence of DKK3. Importantly, these effects were concomitant with β-catenin and YAP activation (Fig. 7g, h), indicating that DKK3 promotes YAP/TAZ signalling by potentiating Wnt signalling in CAFs.
DKK3 disable the negative Wnt regulator Kremen1&2 in CAFs
DKK proteins have been shown to inhibit Wnt signalling by triggering LRP5/6 internalisation through formation of a ternary complex with Kremen1/2 receptors9,10. This is in striking contrast with our observations on the role of DKK3 in CAFs, and underline the functional differences of DKK proteins reported in other systems32,33,34. DKK3 is an unusual member of the family as it does not interact with LRP5/6 but still can interact with Kremen9,10,35. Using HEK293T cells, it has been proposed that DKK3-Kremen interaction negatively affects surface expression of Kremen, which may favour canonical Wnt signalling by still unclear mechanisms35. We first confirmed that Kremen1 but not LRP6 immunoprecipitated with DKK3 in CAFs (Fig. 8a). In addition, DKK3-Kremen1 co-localised to internal structures; however, after DKK3 silencing Kremen1 localised to the cell periphery (Fig. 8b), in an opposite pattern to LRP6 localisation (Fig. 8c). Interestingly, DKK3 expression was inversely associated to changes in Kremen1&2 protein expression (Fig. 8d). mRNA levels of Kremen1/2 were not altered after Dkk3 silencing (Supplementary Figure 8e), suggesting a post-translational regulation. Cycloheximide-chase assays confirmed that Kremen1 was less stable in WT-CAFs than in Dkk3-null CAFs whereas LRP6 stability was increased (Fig. 8e). Our data suggested that disabling Kremen activity may restore LRP6 functions in the absence of DKK3 expression. In agreement, silencing Kremen receptors in Dkk3-null CAFs (Fig. 8f) increased their abilities to remodel gels (Fig. 8g) and promoted cancer cell growth and invasion (Fig. 8h, i). These changes were associated with concomitant re-localisation of LRP6 to the cell surface (Fig. 8j) and reactivation of YAP/TAZ and β-catenin signalling (Fig. 8f, k). Conversely, knocking-down Kremen1&2 in DKK3-expressing CAFs had no functional consequences (Supplementary Figure 8i).
Overall, our mechanistic analyses support a model whereby DKK3 stabilises the cell-surface levels of LRP6 by uncoupling LRP6 from the Kremen-mediated internalisation machinery. In turn, DKK3-mediated LRP6 regulation leads to activation of β-catenin and YAP/TAZ, with the latter being the main mediator of DKK3 functions (Fig. 9).
Secreted DKK proteins are emerging as unpredicted tumour-promoting factors due to their immune-suppressive activities32,34,36. Here, we demonstrate that the family member DKK3 can also promote aggressive behaviours in CAFs in a cell autonomous manner. DKK3 reduces YAP/TAZ degradation by potentiating Wnt signalling, thus acting in parallel to YAP/TAZ regulation via mechanotransduction7,37. DKK3 also activates β-catenin in CAFs and we document that cancer stroma and CAFs present increased β-catenin activity. However, inhibition of β-catenin by RNAi did not affect CAF-mediated ECM remodelling, cancer cell growth and invasion. Further studies are required to determine if β-catenin modulates other pro-tumorigenic functions in CAFs such as potentiating the tumour initiating capacity of cancer cells or immune suppression12. The role of DKK3 as a positive regulator of canonical Wnt is in contrast to the well documented role of other DKK family members. Here, we provide mechanistic insights into these differences. Contrary to what has been shown for DKK1 and DKK2, we show that DKK3 does not bind LRP6 co-receptor in CAFs, and therefore cannot fulfil the bona fide antagonist role of DKK proteins in canonical Wnt signalling. By contrast, we demonstrate that DKK3 perturbs the negative regulator Kremen and enhances Wnt signalling via LRP6.
Whilst several CAF effectors have been identified, an unanswered question is how the coordination of different signalling pathways involved in their control is established. Our results suggest that HSF1-dependent DKK3 upregulation could be a response of stromal fibroblasts to stresses found in the tumour microenvironment. In turn, DKK3 expression promotes fibroblast activation as it sustains crucial activating pathways. Thus, DKK3 expression potentiates canonical Wnt signalling, leading to reduced YAP/TAZ degradation. This leads to YAP/TAZ activation, ECM stiffening and the generation of aggressive tumour microenvironments. We propose that this may be a mechanism for tumour-promoting CAFs to be selected as tumour progresses and a plausible mechanism explaining its significant enrichment in cancer stroma. Further work is still required to understand the processes modulating HSF1 activity in CAFs, and whether the axis HSF1-DKK3-YAP is also relevant in other systems.
To conclude, we identify a key role of DKK3 in tumour stroma. It is required for many of the pro-tumorigenic functions of CAFs, including matrix stiffening and cancer cell growth and invasion. High levels of DKK3 in the stroma are sustained by HSF1, potentiate Wnt signalling and reduce YAP/TAZ degradation. Thus, DKK3 acts as a crucial integrator of key molecular players modulating the emergence of tumour-promoting phenotypes in CAFs.
Transgenic FVB/n mice expressing the Polyoma Middle T antigen oncogene under the Mouse Mammary Tumour Virus promoter (MMTV-PyMT)38 were used for tumour cell isolation and FACS analysis. Wild-type FVB/n and CD-1 Nude mice (Charles River) were used as recipients for tumours. All animals were kept in accordance with UK regulations under project license PPL80/2368.
Established murine fibroblasts from FVB/n normal mammary glands (NFs) and MMTV-PyMT mammary carcinoma (CAFs) have been previously described7,39. Human fibroblasts from normal mammary glands and breast carcinomas were provided by Julia Tchou (University of Pennsilvania, USA)40 and Clare Isacke (Institute of Cancer Research, UK). Human fibroblasts from rectal carcinomas (RC11), colon carcinomas (CAF25) and adjacent normal tissue (NAD-D) were provided by Danijela Vignjevic (Institute Curie, France). Human fibroblasts from ovarian carcinoma (EOC.TIL.04) were provided by Marco Donia (University of Copenhagen, Denmark). Human CAFs from cervical (Cer-CAF) squamous cell carcinoma have been previously described7. Human primary fibroblasts were expanded and immortalised using hTERT virus (pCSII vector backbone) followed by selection with hygromycin. All resulting fibroblast populations were assessed for fibroblast and CAF marker expression and thoroughly characterised7. All fibroblasts were cultured in DMEM (Sigma), GlutaMax (Gibco), 10% FBS, 1% insulin-selenium-transferrin (ITS, Gibco). MMTV-PyMT TS1 murine breast cancer cells were used in most assays and to generate tumours in syngeneic FVB/n mice. Human breast cancer cell line BT20 was cultured in MEM plus GlutaMAX and 10% FBS. Colon cancer cell line HCT116 was grown in McCoy 5 A (with 1% GlutaMAX and 10% FCS). Ovarian cancer cell line SKOV3 was grown in RPMI with 1% GlutaMAX, 10% FBS and 1% Pyruvate. All cell lines were grown in a standard humidified 5% CO2 incubator at 37 °C and tested negative for mycoplasma infection with MycoAlertTM (Lonza). CAFs and cancer cell lines were fluorescently labelled using the following lentiviral vectors, as indicated: EGFP-CAAX pCSII-IRES2-hygro, ECFP-CAAX pCSII-IRES2-hygro, ORANGE-CAAX PCSII-IRES2-hygro, mCherry-CAAX pCSII-IRES2-hygro and pCSII-IRES2-blasti-eGFP.
cDNA, RNAi and reagents
DKK3 (a kind gift by Peter Berger, University of Innsbruck) was ectopically expressed in CAF/NFs lines using a pLENTI6 lentiviral vector system. shRNAs targeting human DKK3 were cloned into the lentiviral vector pLKO.1-TRC-cloning vector (Addgene, Plasmid #10878) at AgeI/EcoRI restriction sites according to the online Addgene protocol (www.addgene.org/tools/protocols/plko). shRNA sequences were obtained from the GPP Web Portal (Broad Institute, http://portals.broadinstitute.org/gpp/public/). A list of the respective shRNA sequences can be found in Supplementary Table 3. siRNAs were purchased from Dharmacon and are listed in the Supplementary Table 4. For in vitro treatments the following growth factors and drugs were used: recombinant human DKK3 (R&D, 1118-DK-050), XAV939 (Sigma, X3004), Cycloheximide (Sigma, C7698), Wnt3a (R&D, 5036-WN-010) and Wnt5a (R&D, 645-WN-010). Lentiviral plasmid for expression of mutant YAP was 5SA-YAP1-YFP vector (kind gift of Eric Sahai, Crick Institute).
Generation of CRISPR knockout cell lines
The CRISPR plasmid U6-gRNA/CMV-Cas9-GFP containing a guide RNA targeting mouse DKK3 was purchased from Sigma (MM0000346166). CAF1 cells (CAF-WT) were transfected with the plasmid using Lipofectamine (Life Technologies) following manufacturer’s instructions and GFP positive cells were single sorted into 96 well plates after 24 h. GFP-negative clones were also sorted as controls (e.g. WT.9). Individual cell clones were expanded and the DKK3 locus targeted by CRISPR was sequenced for knockout validation. In addition, loss of DKK3 protein expression was also confirmed by immunoblotting to generate DKK3-null CAFs (CAF-KO). DKK3-null clones were infected with DKK3-expressing lentivirus to generate KO-CAFs re-expressing DKK3 (CAF-KO-REC).
CAF-derived conditioned media was generated by culturing a confluent monolayer of cells in DMEM 2.5% or 5% FBS for 48 h. Media was then recovered and filtered through a 0.22 μm filter and used as indicated. For detection of DKK3 in the conditioned media, 50 μL of StrataClean resin (Agilent Technologies UK Ltd, 400714) were added to 1 mL of media. Each sample was vortexed for 1 min, left at RT for 1 min and spun 15,000× g for 5 min at 4 °C. The supernatant was then removed and the beads were prepared in protein sample buffer to be resolved in SDS-PAGE.
Fibroblasts were seeded at 60% confluency and transfected using DharmaFECT 1 (Dharmacon) for siRNA (100 nM final concentration), and Lipofectamine (Life Technologies) for plasmids following manufacturer’s instructions. Cell lines stably expressing cDNA (DKK3 or GFP/CFP/mCherry) or shRNAs were generated by lentiviral infection followed by puromycin selection for 2 weeks (2 μg mL−1). Alternatively, fluorescent-labelled cells were sorted by FACS.
To assess force-mediated matrix remodelling, 7.5 × 104 murine fibroblasts or 3–5 × 104 human fibroblasts were embedded in 120 µL of a mixture of collagen I (BD Biosciences) and ECM gel mixture (Sigma) yielding a final collagen concentration of ~4.6 mg mL−1 and a final ECM gel mix concentration of ~2.2 mg mL−1 (Collagen-rich matrix hereafter). Once the gel was set, cells were maintained in fibroblasts medium. Gel contraction was monitored daily by scanning the plates. Unless stated otherwise, the gel contraction value refers to the contraction observed after 2 days. To obtain the gel contraction value, the relative diameter of the well and the gel were measured using ImageJ software, and the percentage of contraction was calculated using the formula 100 × (well area − gel area) / well area. When indicated, gels were fixed in 4% paraformaldehyde (PFA) for 16 h and processed for immunofluorescence. Backscattered light (reflectance) was collected to image the matrix surrounding cells. Alternatively, collagen second-harmonic generation (SHG) signal was acquired by exciting with an 880 nm pulsed Ti–Sapphire laser and acquiring emitted light at 440 nm using a Leica SP8 microscope.
The 3D co-culture spheroid assay was adapted from Dolznig et al19. In short, cancer cells were seeded in low numbers (200 cells per well TS1-mCherry, 50 cells per well HCT116-mCherry, 150 cells per well BT20-GFP and SKOV3-orange) in ultra-low adherent cell culture plates (Corning) to form spheroids for 48 h. The spheroids were collected and mixed with 1 × 105 fibroblasts. The spheroid and fibroblast suspension was centrifuged at 200× g for 4 min and the supernatant was carefully removed. The pellet was first re-suspended in 30 µL of medium and then mixed with 270 µL of Collagen-rich matrix. The 300 µL gel containing the spheroids and fibroblasts was seeded in a glass-bottom 3.5 cm MatTek dish. A nylon filter (Nylon NET filters 120 μm, Merck Millipore) with a hole of ~1 cm diameter cut out at the centre was added to prevent excessive gel contraction. The gel was set for 1 h in the incubator at 37 ˚C and cancer cell medium was added. The spheroids were incubated for 5–7 days (3–5 days for studies involving siRNA-transfected CAFs) and then fixed with 4% PFA and either imaged directly or stained additionally with DAPI and phalloidin-TRITC or phalloidin-FITC. Samples were mounted and analysed using a Leica SP8 confocal microscope. Confocal sections were acquired for individual spheroids from bottom to top, z-stack projections were analysed using ImageJ and data analysed in GraphPad Prism. The invasion index was calculated by measuring the total area over which cancer cells had dispersed (including invading and non-invading cells) and the area of non-invading cells (1 – [non-invading area/total area]). To calculate tumour growth index, area covered by cancer cells in each spheroid was calculated.
Matrigel on Top (MOT) co-culture
24-well MatTek glass bottom plates were pre-coated with 100 µL of Matrigel. Next, 6 × 104 CAFs per well were seeded, unless otherwise stated. After 1 h, fluorescently labelled cancer cells (2 × 104 per well) were seeded and allowed to adhere for 4 h. Growth medium was exchanged to DMEM 2.5% FCS and 2% Matrigel and co-cultures were allowed to grow for 48 h, or up to 120 h for the ovarian cells. Co-cultures were then fixed in 4% PFA and analysed by confocal microscopy. Images were analysed using ImageJ and data analysed in GraphPad Prism. To calculate cancer cell growth index, area covered by cancer cells in each field of view was calculated.
Atomic force microscopy
To assess the elastic modulus of gels remodelled by fibroblasts, 75 × 103 fibroblasts were embedded in 100 µL of Collagen-rich matrx and seeded on ultra-low attachment 96-well plate (Costar). Once the gels were set, they were maintained in fibroblast medium. After three days, the elastic modulus of the gels was measured as previously described7. Gels were gently lifted from their well and fixed in the centre of 50 mm glass bottom Petri dishes using cyanoacrylate superglue. Once glued, Leibovitz L-15 medium (Invitrogen) supplemented with 10% FCS was added to the dish. AFM measurements were performed with a JPK Nanowizard-I (JPK instruments, Berlin, Germany) interfaced to an inverted optical microscope (IX-81, Olympus). AFM cantilevers with pyramidal tips (MLCT, Bruker, Karlsruhe, Germany) and nominal spring constants of 0.07 Nm−1 were modified by gluing 35-μm radius glass beads to the cantilever underside with UV curing glue (UV curing, Loctite, UK). Cantilever spring constants were determined prior to modification using the thermal noise method implemented in the AFM software (JPK SPM). Prior to any indentation tests, the sensitivity of the cantilever was set by measuring the slope of force-distance curves acquired on glass regions of the Petri dish. Using the optical microscope, the tip of the cantilever was aligned over regions in the middle of the gel and, for each gel, measurements were acquired in 30–40 locations ~100 μm apart. Force-distance curves were acquired with an approach speed of 5 μm.s−1 until reaching the maximum set force of 3 nN. After the experiment, the elastic moduli were extracted from the force-distance curves by fitting the contact portion of curves to a Hertz contact model. For each force-distance curve, goodness of fit was evaluated by calculating r^2 values and only fits with r^2 > 0.80 were retained for further analyses (representing on average 80% of the acquired force-curves).
Cells were fixed in 4% PFA for 1 h and permeabilised by incubation in PBS 0.2% Triton 100 (Sigma) at RT for 20 min. For HSF1 immunofluorescence, cells were permeabilised by incubation in PBS 0.5% NP-40 (Sigma) at 4 °C for 20 min (twice), in PBS 0.3% Triton 100 (Sigma) at RT for 20 min and in PBS 0.1% Triton 100 at RT for 15 min (twice). Where indicated (non-permeabilised), this step was not performed. Samples were blocked for 60 min at RT in blocking solution: 4% BSA PBS 0.05% Tween20 (Sigma). Then, cells were incubated with primary antibody in blocking solution in a wet chamber overnight at 4 °C. After three washes of 15 min in PBS, secondary antibody in blocking solution was added for 3 h. After three washes of 15 min in PBS, the samples were mounted and analysed using a Leica SP8 confocal microscope. To quantify β-catenin activation by immunofluorescence, for each field of view we calculated the area positive for active-β-catenin staining per cell using Volocity. These values were then normalised to the mean value of CAF-WT (murine CAFs) or control siRNA-transfected cells (human CAFs). To quantify YAP and TAZ activation by immunofluorescence, we measured their nuclear localisation using Volocity. Briefly, we calculated for each cell the total intensity of nuclear HSF1, YAP or TAZ staining (determined by the intensity within the region delimited by DAPI staining) and perinuclear staining (defined as a region encompassing two to seven pixels from the nucleus border). Nuclear/cytoplasmic YAP and TAZ ratios were calculated as the log10 of the total nuclear intensity/mean perinuclear region intensity per cell. For the analysis of human CAFs, nuclear/cytoplasmic YAP and TAZ ratios were normalised to the mean ratio of control siRNA-transfected cells. Antibody description and working dilutions can be found in Supplementary Table 2.
Tissue immunohistochemistry and immunofluorescence
For histological analysis, tumours were dissected into 10% neutral buffered formalin, embedded in paraffin blocks and serial sections were taken. Paraffin-embedded tissue sections were rehydrated before antigen retrieval using pH 6 sodium citrate buffer. After blocking endogenous peroxidase (DAKO peroxidase block), sections were incubated for 1 h at RT with primary antibodies. For DKK3 staining, four antibodies (Abgent AP1523a, R&D AF1118, Sigma HPA011868 and Santa-Cruz H130) were tested for specificity using western blot and cell pellets in control and DKK3-silenced CAFs, and then optimised for TMA analysis. Both Sigma and Santa-Cruz antibodies yielded satisfactory results. Data presented correspond to Santa-Cruz DKK3 antibodies. Sections were incubated with secondary antibodies for 50 min at RT, treated with 3,30-diaminobenzidine and counterstained with haematoxylin. Images were acquired using a Digital Slide Scanner (Hamamatsu Photonics). Images were analysed using Volocity and data analysed in GraphPad Prism. For each staining, positive area was calculated and normalised to the total area for all slices processed. For immunofluorescence, after antigen retrieval sections were incubated with 0.2% Triton X-100 (Sigma) 10% Goat serum (Sigma) blocking solution for 1 h RT. Sections were incubated overnight at 4 °C with primary antibodies diluted in PBS. After washing, the sections were incubated with fluorescent-conjugated secondary antibodies (AlexaFluor) diluted in PBS for 1 h at RT. Confocal images were captured using a Leica SP8 microscope. Quantitative analysis of YAP and β-catenin staining in the tumour stroma was performed using Volocity software. Briefly, fibroblasts were identified using automated threshold based on S100A4 staining. The mean intensities of YAP and β-catenin were then measured. In addition to haematoxylin and eosin staining and Masson’s trichrome staining, antibody description and working dilutions can be found in Supplementary Table 2.
The following tissue microarrays were purchased from US Biomax, Inc: breast cancer (BR1504b), colon cancer (CO2083) and ovarian cancer (OV2001a). Formalin-fixed, paraffin-embedded (FFPE) sections were deparaffinized, blocked with 3% H2O2 and antigen retrieval was performed using a pressure cooker with Dako citrate buffer (pH 6.0). Slides were incubated with DKK3 antibody (DKK-3 H-130, Santa Cruz, sc-25518) for 1 h at RT. Visualisation was achieved with 3,30-diaminobenzidine as a chromogen (Dako Envision + System). Images were acquired using a Digital Slide Scanner (Hamamatsu Photonics). DKK3 staining was quantified using the weighted Histoscore method to give a value of 0–30041.
Tissue digestion for cell isolation and FACS analysis
MMTV-PyMT tumours were minced with a scalpel and digested with a mixture of DNase and Liberase (Roche Diagnostics). On enzymatic digestion, samples were passed through a 100-μm filter. Cells were incubated for 5 min at RT in 2 mL NH4Cl solution (0.8% in H2O) to eliminate red blood cells. Cells were then directly used for FACS staining and sorted with a FACSAria flow cytometer (BD Biosciences). Single cell suspensions of tumour cells were labelled with FITC Rat Anti-Mouse CD45 Clone 30-F11 (BD Pharmingen™, 553079), APC Rat Anti-Mouse CD31 Clone MEC 13.3 (BD Pharmingen™, 561814), PE/Cy7 anti-mouse CD326 (Ep-CAM) (BioLegend, 118215) and PE anti-mouse CD140a (PDGFRa) (BioLegend, 135905). For cell sorting, four populations (CD45+ for immune cells, CD31+ for endothelial, EPCAM+ for epithelial and PDGFRA+ for CAFs) were collected and processed for RNA extraction and qRT-PCR.
For proliferation analyses with conditioned media, TS1 cancer cells were seeded at 5,000 per well in a 24-well plate in duplicates and incubated with media containing 5% FBS (vehicle) or 5% FBS plus 100 ng mL−1 recombinant DKK3 (rDKK3), or with CAF conditioned medium (5% FBS) for 72 h. Where indicated, blocking antibody for DKK3 (R&D AF1118) or isotype control (R&D AB-108-C) were added at 1 µg mL−1. AlamarBlue® assay was used according to manufacturer’s instructions to compare cell growth and viability.
Scratch Wound healing assay
TS1 cancer cells were cultured to a confluent monolayer in 24-well plates. A straight scratch was made on the cell layer using a 200 µL tip across the centre of the well. Cell debris was removed by washing twice with PBS and cells were incubated with media containing 5% FBS (vehicle) or 5% FBS plus 100 ng mL−1 recombinant DKK3 (rDKK3), or with CAF conditioned medium (5% FBS). Where indicated, blocking antibody for DKK3 (R&D AF1118) or isotype control (R&D AB-108-C) were added at 1 µg mL−1. Cell migration was monitored for 40 h using live cell imaging in the IncuCyte® (Essen Bioscience) in a standard humidified 5% CO2 incubator at 37 °C. The wounded area at start and end of experiment was measured using ImageJ. Wound closure was calculated as the percentage of healed area (area at t0 minus area at t40) to the starting wounded area.
For Kremen1 and DKK3 co-inmunoprecipitations, CAFs expressing empty vector of Flag-DKK3 were lysed in immunoprecipitation lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton-X-100, 10% glycerol, 2 mM EDTA, 25 mM NaF and 2 mM NaH2PO4) containing protease and phosphatase inhibitor. Lysates were pre-cleared using G-sepharose, incubated with anti-Flag antibody (rabbit) or IgG immobilised on G-sepharose beads at 4 °C overnight. Beads were then washed five times with lysis buffer and eluted with 20 μL of 2x SDS sample buffer.
Protein lysates and immunoprecipitants were processed following standard procedures. Enhanced-chemiluminescence signal was acquired using an Azure Biosystems c600. Exposures within the dynamic range were quantified by densitometry using ImageJ. Antibody description and working dilutions can be found in Supplementary Table 2. Immunoblot images in Figures and Supplementary Figures show molecular weight markers in kDa. Supplementary Figure 9 shows the uncropped images corresponding to the panels displayed in the main figures.
Luciferase reporter assays
The following plasmids were used for luciferase reporter assays: TEAD‐reporter construct (8xGTIIC-luciferase, Addgene, Plasmid #34615), TOP‐reporter construct (M50 Super 8x TOPFlash, Addgene, Plasmid #12456) and CMV-Renilla (pGL4.75[hRluc/CMV], Promega). CAFs were seeded in 6-wells plates at 70% confluency. Cells were transfected with 2 µg TOPFlash or TEAD‐reporter and 100 ng of CMV-Renilla cDNA constructs using Lipofectamine 3000 according to the manufacturer’s instructions. Cells were lysed 48 h after transfection in 100 μL of lysis buffer (Promega). Aliquots of the cell lysates were used to read luciferase emission using Dual-Glo® Luciferase Assay System (Promega) according to the manufacturer’s instructions. Luciferase activities were normalised to Renilla activity.
Generation of syngeneic orthotopic tumours
MMTV-PyMT TS1 murine breast cancer cells were used to generate tumours in wild-type FVB/n. Briefly, 106 TS1 cells and 3 × 106 CAF (WT, KO.9 or KO.9-REC) were suspended in 100 μL of PBS:Matrigel (50:50) and injected subcutaneously into 6–8 week old females. Tumour size was measured every other day using callipers. To calculate tumour volume the formula V = (length × width2) / 2 was used. Mice were killed once tumours reach the maximum allowed size.
The implantation of an orthotopic mammary imaging window in CD-1 nude mice was performed as previously described42. Forty-eight hours after surgical implantation of the imaging window, a mixture of 1 × 106 CAFs (GFP) and 4 × 105 TS1 (CAAX-mCherry) cancer cells in a PBS:Matrigel solution (50:50) were injected under the window. Intravital 2-photon imaging was performed at specified time-points using a Leica SP8 microscope. Briefly, mice were anaesthetised and tumours were excited with an 880 nm pulsed Ti–Sapphire laser and emitted light acquired at 440 nm (collagen second-harmonic generation, SHG) and 530 nm (GFP). In addition, a mCherry signal was acquired sequentially using confocal microscopy. During ~10-min intervals, 5 to 8 different regions were imaged simultaneously for 2 h for each tumour. In each region, a z-stack of 4–5 images (approximately 50 µm deep on average) was taken at a spacing of 10 μm between images, resulting in a time-lapse three-dimensional z-series for analysis. To analyse the collagen content, mean intensity of SHG signal of the top confocal plane for each z-stack was used. Moving cells were defined as those that moved 10 μm or more during the length of each movie.
RNA isolation and qRT-PCR
To obtain RNA from the different fibroblast populations, RNA was isolated using EZNA Total RNA Kit 1 (Omega Bio-tek). Reverse transcription was performed using Precision NanoScript 2 Reverse-Transcription-kit (PrimerDesign) and qPCR using PrecisionPLUS 2x qPCR MasterMix with ROX and SybrGreen (PrimerDesign). Expression levels of indicated genes were normalised to the expression of Gapdh, Rplp1 or Lamc2. Sequences of the oligonucleotides used for qRT-PCR are described in the Supplementary Table 5. For array analysis, RNA was isolated as described and processed in collaboration with Eurofins Genomics (GeneChip Mouse Gene 2.0 ST Array—details available on reasonable request).
7–10 × 106 NF or CAF at subconfluency were fixed in 1% formaldehyde and sonicated using the Bioruptor Pico sonication device (Diagenode; B01060001) using 15 cycles (30 s on; 30 s off) at maximum intensity. Purified chromatin was then separated for: (i) immunoprecipitation using Dynabeads Protein G (Life Technologies: 10003D) coated with 8 µg and 4 µg of HSF1 antibody (ThermoFisher: RT-405-P, and Cell Signalling: 4356 S, respectively) per ChIP experiment; (ii) non-immunoprecipitated chromatin, used as Input control; and (iii) assessment of sonication efficiencies using a 1% agarose gel. For the quantitative PCR briefly, reactions were carried out in 10 μL volume containing 5 μL of Sybergreen mix (Applied Biosystems; 4472918), 0.5 μL of primer (5 μM final concentration), 2.5 μL of genomic DNA and 2 μL of DNAse/RNAse-free water. A three-step cycle programme and a melting analysis were applied. The cycling steps were as follows: 10 s at 95 °C, 30 s at 60 °C and 30 s at 72 °C, for 40 cycles. Enrichment of the immunoprecipitated sample was confirmed using positive and negative controls. The exact loci of the primers are as follows: negative control (gene desert): chr6:116908976–116909177; Dkk3 Promoter (P): chr7:112159485–112159638; Dkk3 Enhancer (E): chr7:112183116–112183344; Rilpl, within the gene (positive control): chr5:124510011–124510190.
Gene expression analyses of clinical datasets
Gene expression analyses of human tumour stroma were retrieved from NCBI Gene Expression Omnibus (GEO). Datasets are described in Supplementary Figure 1a and include: Finak (GSE9014, Breast), Karnoub (GSE8977, Breast), Yeoung (GSE40595, Ovary), Nishida (GSE35602, Colon), Saadi (GSE19632, Aesophagus), Costea (GSE38517, Oral SCC), Navab (GSE22863, Lung) and Sherman (GSE43770, Pancreas). Additional datasets include: GSE14548 (Breast cancer epithelia and stroma), GSE20086 (human breast NF and CAFs), GSE70468 (human colon NF and CAFs) and GSE35250 (human ovarian NF and CAFs). In addition, the dataset describing the expression profiles of cell populations purified from human colorectal cancer (GSE39396) was also used. For Affimetrix-based arrays, probe-to-gene mapping was performed using Jetset43; for the rest of the arrays, highest variance probes were selected. Probes mapping DKK genes used for each array can be found in Supplementary Figure 1a. Unless otherwise stated, expression values for each gene were z-score normalised. For the analyses of YAP/TAZ and β-catenin signatures in tumour stromal datasets, we first generated CAF-specific YAP/TAZ and β-catenin signatures. Genes significantly downregulated in murine CAF1 after transfection with YAP/TAZ siRNA or XAV939 (β-catenin inhibitor) treatment were identified. In addition, we sought for genes specifically expressed by FAP-positive CAFs in human tumours using the GSE39396 dataset. This dataset describes the expression profiles of cancer cells, immune cells, endothelial cells and CAFs purified from human colorectal cancer. We identified genes significantly upregulated more than 1.5-fold in FAP-positive samples when compared to the rest of the samples. Genes from the YAP/TAZ and β-catenin signatures that overlapped with the genes in the FAP-positive signature were selected into CAF-specific signatures; the rest were selected into CAF-unspecific signatures. To calculate the gene-signature score in each sample, we used single sample Gene Set Enrichment Analysis (ssGSEA)44 Projection Software from the GenePattern platform developed by the Broad Institute of MIT and Harvard (USA) and available at GenePattern (www.genepattern.broadinstitute.org), following the programs guidelines (log normalisation; weighting exponent 0.75). This analysis calculates separate enrichment scores for each pairing of sample and gene set. Each ssGSEA enrichment score represents the degree to which the genes in a particular gene set are co-ordinately up- or downregulated within a sample. ssGSEA scores were z-score normalised. For analyses of HSF1 signature, a similar approach was performed. The top 200 genes downregulated between wild-type and Hsf1-null mouse embryonic fibroblasts (GSE56252) were identified and genes also present in the FAP-positive signature were selected to generate the HSF1 CAF-specific signature.
Enrich database analysis
To identify putative transcription factors (TFs) regulating DKK3 expression we used the Enrich database14,15 [http://amp.pharm.mssm.edu/Enrichr/]. We queried for DKK3 gene and retrieved the ChEA 2016 dataset to identify possible TFs that bind the DKK3 promoter, obtaining a list of 58 TFs (Supplementary Data 1). In addition, we retrieved the TF-LOF Expression from GEO dataset to obtain a list of TFs whose loss of function has been associated to changes in DKK3 expression (Supplementary Table 6).
Analysis of clinical relevance of DKK3 expression was assessed using publicly available data from the Kaplan–Meir Plotter platform for breast and ovarian cancer45,46 (version 2014 and 2015, respectively), as well as the GSE17538 dataset for colorectal cancer47. Probe-to-gene mapping was performed using Jetset43. For survival analysis of ovarian cancer (progression-free survival), the highest quartile of gene expression was used to dichotomise the different tumours into high and low groups. Survival analyses for breast (recurrence-free survival) and colon (disease-specific survival) datasets, tumours were split into high and low based on median expression, respectively. For breast cancer, further analyses of recurrence-free survival and distant-metastasis-free survival were performed based on ER-status and molecular subtype. Breast cancer disease-free survival analysis based on DKK3 expression in the tumour microenvironment was performed using the Finak Dataset48. Only ER-negative patients were analysed. Mean DKK3 expression was used to split tumours into high and low expression groups.
Gene-set enrichment analyses (GSEA)
Array data were processed and analysed using the Gene-set enrichment analysis software, developed by the Broad Institute of MIT and Harvard (USA) and available at www.broadinstitute.org, following the programme guidelines. The specific settings applied in all analyses are: Number of Permutations (1000), Permutation Type (Gene set), Enrichment statistic (Weighted), and Metric for ranking genes (t Test). Values represent the False Discovery Rate (-log2) and the Nominal Enrichment Score (NES) of each gene set. The list of the specific gene sets analysed and their sources are available in Supplementary Data 2.
Statistical analyses were performed using GraphPad Prism (GraphPad Software, Inc.). When n permitted, values were tested for Gaussian distribution using the D’Agostino-Pearson normality test. For Gaussian distributions, paired or unpaired two-tailed Student’s t-test and one-way ANOVA with Tukey post-test (for multiple comparisons) were performed. For non-Gaussian distributions, Mann–Whitney’s test and Kruskal–Wallis test with Dunn’s post-test (for multiple comparisons) were performed. Unless stated otherwise, mean values and standard errors (SEM) are shown. Survival curves were estimated based on the Kaplan–Meier method and compared using a log-rank test. P values of less than 0.05 were considered statistically significant. Where indicated, individual p values are shown; alternatively the following symbols were used to describe statistical significance: *P < 0.05; **P < 0.01; ***P < 0.001; #P < 0.0001; n.s., non-significant.
Further information on experimental design is available in the Nature Research Reporting Summary linked to this article.
Gene expression datasets of NF control or overexpressing DKK3, and CAF1 after transfection with control or DKK3 siRNAs are available at the NCBI GEO under GSE114056 [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc = GSE114056]. The rest of the datasets generated and/or analysed during the current study are available from the corresponding author upon reasonable request.
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We thank Clare Isacke, Marjan Iravani and Sarah Tang in the Breast Cancer Now Research Centre at the Institute of Cancer Research for kindly providing human breast CAFs, and Danijela Vignjevic at Institute Curie for providing human colon CAFs. In addition, we thank Sebastian Guettler, Peter Berger, Robert Kypta, Christoff Niehrs and Erik Sahai for providing us with plasmids; Afshan McCarthy for providing MMTV-PyMT tumours; Johanna Joyce, Rachael Natrajan, Pascal Meier, Udai Banerji and Richard Houlston for providing cancer cell lines; Jacco van Rheenen for training on mammary window imaging; Frances Daley and Anne Lowe for help with immunohistochemistry; Fredrik Wallberg and members of the FACS/Light microscopy Unit for assistance; members of the Biological Services Unit for help with mouse experiments; and Anguraj Sadanandam for assistance with bioinformatics analyses. We also thank lab members for help and advice throughout this work. This work was funded by Worldwide Cancer Research (Grant 15–0273) and the Institute of Cancer Research. F.C. is also funded by Cancer Research UK (C57744/A22057) and the Ramon y Cajal Research Program (MINECO, RYC-2016–20352). Spanish funding to F.C. is partially supported by the European Regional Development Fund.
The authors declare no competing interests.
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Ferrari, N., Ranftl, R., Chicherova, I. et al. Dickkopf-3 links HSF1 and YAP/TAZ signalling to control aggressive behaviours in cancer-associated fibroblasts. Nat Commun 10, 130 (2019). https://doi.org/10.1038/s41467-018-07987-0
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