Fig. 1 | Nature Communications

Fig. 1

From: Single-cell microRNA-mRNA co-sequencing reveals non-genetic heterogeneity and mechanisms of microRNA regulation

Fig. 1

Experimental workflow. a Overall strategy for profiling miRNA and mRNA from the same single cells using half-cell genomics. It involves cell lysis, half-cell split, followed by small RNA & large RNA library preparation. b For miRNA library preparation, a pre-adenylated (APP) 3′ adaptor was used to ligate to the 3′ end of miRNA molecules, followed by digestion of unreacted 3′ adaptor, ligation with 5′ adaptor, RT and PCR amplification. c For mRNA library preparation, first-strand cDNA synthesis was primed by the 3′ SMART-Seq CDS Primer IIA. Template switching at the 5′ end of transcript was performed using the SMART-Seq v4 oligonucleotides. After PCR amplification, cDNA was fragmented using Illumina’s Tagmentation process

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