Fig. 4: | Nature Communications

Fig. 4

From: Fibril formation and therapeutic targeting of amyloid-like structures in a yeast model of adenine accumulation

Fig. 4

Addition of TA rescues the toxic effect observed in the adenine salvage mutant. a WT, aah1Δ, apt1Δ, and aah1Δapt1Δ strains were serially diluted and spotted on SD medium without adenine (−ADE) or on SD media containing 2 mg/L adenine with or without various concentrations of TA, as indicated. b Dose–response curve for aah1Δapt1Δ cells in SD medium containing adenine and TA at different concentrations. The percentage of growth represents the growth with TA compared to the growth without TA. c Flow cytometry analysis of WT and aah1Δapt1Δ cells under the indicated conditions following ProteoStat staining. *P < 0.01 (Student’s t-test). Values are the mean ± s.d. of three experiments. TA concentration was 0.5 mM. d Representative confocal microcopy images of aah1Δapt1Δ cells under the same conditions as in (c). Cells were visualized using DIC microscopy. Scale bars are 50 and 5 μm for the lower (first line) and higher resolution (second line) images