Fig. 3: | Nature Communications

Fig. 3

From: Fibril formation and therapeutic targeting of amyloid-like structures in a yeast model of adenine accumulation

Fig. 3

In vivo formation of amyloid-like structures upon adenine feeding. a and b Flow cytometry analysis (a) and representative confocal and differential interference contrast (DIC) images (b) of WT and aah1Δapt1Δ cells under the indicated conditions using ProteoStat staining. *P < 0.01 (Student’s t-test). Values are the mean ± s.d. of three experiments. Scale bars are 50 and 5 μm for the lower (first and third lines) and higher resolution (second and fourth lines) images. c and d Z-stack followed by 3D reconstruction (c) and projection of a single section (d) of aah1Δapt1Δ cells using ProteoStat. The analysis was performed using the Imaris software. Scale bar in (d) is 1 µm. e Representative image of aah1Δapt1Δ cells double-stained with Hoechst and ProteoStat. Cells were visualized using DIC microscopy. Scale bar is 1 µm. f Representative confocal and differential interference contrast (DIC) images of WT and aah1Δapt1Δ cells under the indicated conditions. Cells were fixed and subjected to indirect immunofluorescence using a polyclonal antibody raised against adenine amyloid-like assemblies, designated as Anti-ADEaf (=amyloid fibrils). Scale bar is 5 µm