Fig. 1: | Nature Communications

Fig. 1

From: Fibril formation and therapeutic targeting of amyloid-like structures in a yeast model of adenine accumulation

Fig. 1

Sensitivity of the salvage mutant to adenine feeding. a Wild-type (WT), aah1Δ, apt1Δ, and aah1Δapt1Δ strains were serially diluted and spotted on SD complete medium containing 20 mg/L adenine (SD-com) or on SD medium without adenine (−ADE). b Growth curves of WT and aah1Δapt1Δ cells in SD media with or without adenine (ADE). SD-com is denoted as +ADE to emphasize the presence of adenine in the standard yeast growth medium. c WT, aah1Δ, apt1Δ, and aah1Δapt1Δ strains were serially diluted and spotted on SD medium with various concentrations of adenine, ranging from 40 mg/L (~300 µM) to 5 µg/L (~0.0375 µM), or without adenine (−ADE). d Cells were grown in the presence of different concentrations of adenine, ranging from 40,000 µg/L (~300 µM) to 0.038 µg/L (~0.029 nM), in SD medium and the absorbance at 600 nm was measured at the logarithmic phase. The results were fitted using a four-parameter logistic equation (4PL), R2 > 0.99. Inset shows the absorbance of the WT at 600 nm. e Intracellular concentration of adenine determined using GC–MS. WT and aah1Δapt1Δ cells were grown in SD media in the absence (−ADE) or presence (+ADE) of adenine and the metabolites were extracted. *P < 0.01 (Student’s t-test). Values are the mean ± s.d. of three experiments