Fig. 5 | Nature Communications

Fig. 5

From: Evolution of a General RNA-Cleaving FANA Enzyme

Fig. 5

Cleavage of a chimeric DNA/RNA substrate contain a single unpaired ribo-G residue. a Predicted secondary structure showing FANAzyme NGS12-7 in complex with the substrate. b Substrate cleavage profile under single-turnover conditions. Representative gel image (top) and kinetic profile (bottom). ([Fz] = 2.5 µM, [S] = 0.5 µM) n = 3, error bars represent s.d. c Substrate cleavage under multiple-turnover conditions. ([Fz] = 10 nM, [S] = 0.5 µM). All reactions were carried out in trans in 50 mM CHES (pH 8.5) containing 200 mM NaCl and 25 mM MgCl2 at 24 °C. S substrate, P cleavage product

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