Fig. 2 | Nature Communications

Fig. 2

From: ATP-dependent membrane remodeling links EHD1 functions to endocytic recycling

Fig. 2

Membrane binding and ATPase activity of EHD1. a Representative gel showing results from a sedimentation assay with EHD1 added to liposomes of increasing PS content and spun down to separate the supernatant (S) and pellet (P) fractions. M denotes the marker lane. b Bound fraction and ATPase activity of EHD1 with liposomes of increasing PS content. Data represent mean ± SD for N = 3. c Bound fraction and ATPase activity of EHD1(F322A) with liposomes of increasing PS content. Data represent mean ± SD for N = 3. d Bound fraction and ATPase activity of EHD1 on liposomes of the indicated compositions. Data represent mean ± SD for N = 3. e Liposome-bound fraction of EHD1 with increasing PS with different nucleotides (mean ± SD, N = 3 for Apo, N = 3 for ADP, N = 3 for AMP-PNP, N = 5 for ATP-γ-S). Statistical significance was assessed using an unpaired two-tailed t-test and **P = 0.003, ***P = 0.0001

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