Fig. 8 | Nature Communications

Fig. 8

From: A non-canonical BRD9-containing BAF chromatin remodeling complex regulates naive pluripotency in mouse embryonic stem cells

Fig. 8

BRD9 is dispensable for regulating the naive pluripotency network in 2i. a Time course experiment assessing mouse ES cell number cultured in 2i conditions (MEK inhibitor PD0325901 and GSK3 inhibitor CHIR99021), after treatment with DMSO or I-BRD9 at either 3 or 10 µM. Error bars represent one standard deviation from the mean of three biological replicates. b Bar graph showing the fold change in pluripotency gene expression after 72 h of treatment with I-BRD9 or JQ1 of ESCs cultured in serum/LIF (S/L) or 2i. Mean of two technical replicates; error bars represent one standard deviation from the mean. Source data are provided as a Source Data file. c Immunoblotting analysis of ESC nuclear lysates after 72 h of treatment with I-BRD9 or JQ1 of ESCs cultured in S/L or 2i. Molecular weights from ladders are indicated. Source data are provided as a Source Data file. d Quantification of the indicated proteins in c, normalized to TATA-binding protein (TBP) loading control then normalized to values in S/L DMSO. Dotted line is y = 1, which represents S/L DMSO. e Model of BRD4-mediated recruitment of BRD9/GBAF to sites annotated to naive pluripotency genes

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