Table 1 Calculated EC50 values for Dox, TCO-Dox and activated Dox against each cell line

From: Synergistic enzymatic and bioorthogonal reactions for selective prodrug activation in living systems

Drug EC50, HeLa EC50, Saos-2 EC50, CCC-HEH-2 EC50, HUVECsa
Dox 0.732 0.425 0.084 0.133
TCO-Dox 6.793 7.569 6.169 5.808
Activated Doxb 0.029 0.0001 0.404 0.272
  1. aEC50 values are determined the 72 h cytotoxicity of each drug. The units of EC50 are in μM.
  2. bActivated Dox indicates that each cell line was pre-incubated with 100 μL of 500 μM 2 for 6 h and incubated with 200 μL of TCO-Dox at different concentrations for 72 h.