Fig. 3 | Nature Communications

Fig. 3

From: Synergistic enzymatic and bioorthogonal reactions for selective prodrug activation in living systems

Fig. 3

Quantification and location of the intracellular self-assembly in live cells. a Flowchart showing the cell culture conditions. Each group of cells was treated with different concentrations (100–500 μM) of 2 for a range of durations (1, 2, 4, 6, 15 and 24 h), washed with PBS and incubated with 100 μL of 50 μM TCO-CMR. b Fluorescence intensity of restored coumarin in HeLa cells for each incubation condition with 100–500 μM 2 for 1, 2, 4, 6, 15 and 24 h, respectively (n = 6). c Comparison of the fluorescence intensity from restored coumarin in HeLa cells and HUVECs. All cells were pre-treated with 100–500 μM 2 for 6 h, washed with PBS and incubated with 100 μL of 50 μM TCO-CMR for 24 h (n = 6). Error bars indicate standard deviation. d Confocal images showing the distribution of liberated coumarin (blue) and the overlap with ER tracker (red). Scale bar, 50 μm

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