Fig. 5 | Nature Communications

Fig. 5

From: Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease

Fig. 5

Cellular contributors to cfDNA in islet transplant recipients. a Deconvolution results for pooled sample of cfDNA from five patients, 1 h after islet transplantation. The patients present a noticeable amount of pancreas-derived cfDNA (typically absent in healthy donors). Cell types contributing <1% were included in “Other”. b Same as a, expressed as absolute levels of cfDNA (genome equivalents per ml plasma). Also shown is the prediction for a healthy individual. c Inferred amount of cfDNA from all three pancreas cell types for three individuals prior to, 1 h after, and 2 h after islet transplantation. Error bars: SD, estimated using Bootstrapping. d Comparison of pancreatic cfDNA estimations using deconvolution (y-axis) to results of targeted insulin promoter methylation assay (x-axis). Pearson’s r = 0.996, p-value = 1.6e−8. e Same as c, using a reference atlas with whole pancreas methylome, instead of purified pancreas cell types. Here, deconvolution fails to identify pancreatic cfDNA in recipient 1

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