Fig. 4 | Nature Communications

Fig. 4

From: Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease

Fig. 4

Cellular contributors to cfDNA in healthy individuals. a Predicted distributions of contributors to circulating cfDNA, averaged across eight sample pools of healthy donors. Contributions smaller than 1% were included in “Other”. b Deconvolution results for eight sets of pooled DNA samples, expressed as absolute levels of DNA (genome equivalents/ml plasma, derived by multiplying the fraction contribution of each tissue by the total amount of cfDNA in 1 ml plasma). Shown are contributions larger than 1%. Young, 19–30 years old; Old, 67–97 years old (pool average > 75 yr). c Comparison of estimated proportion of various cell types in healthy plasma samples (blue) vs. leukocytes (orange), as predicted by deconvolution. Shown, from left, are the contributions of erythrocyte progenitor cells, vascular endothelial cells and hepatocytes, all of which are not expected in leukocyte samples. Also shown are the predicted contributions of lymphocytes, that represent a large fraction of leukocyte cell population. Shaded boxes mark 95% confidence interval of the sample mean. d Deconvolution of whole blood methylomes (not plasma), showing excellent correlation (Pearson’s r = 0.985, p < 2e−16) between the estimated proportions of monocytes, neutrophils and lymphocytes and the actual proportions of these cells obtained via standard complete blood count (CBC) for each sample

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