RORγt controls the differentiation of TH17 cells, which are mediators of autoimmune conditions such as experimental autoimmune encephalomyelitis (EAE). RORγt also regulates thymocyte development and lymph node genesis. Here we show that the function of RORγt is regulated by its sumoylation. Loss of Sumo3, but not Sumo1, dampens TH17 differentiation and delays the progression of thymic CD8+ immature single-positive cells (ISPs). RORγt is SUMO3-modified by E3 ligase PIAS4 at lysine 31 (K31), and the mutation of K31 to arginine in mice prevents RORγt sumoylation, leading to impaired TH17 differentiation, resistance to TH17-mediated EAE, accumulation of thymic ISPs, and a lack of Peyer’s patches. Mechanistically, sumoylation of RORγt-K31 recruits histone acetyltransferase KAT2A, which stabilizes the binding of SRC1 to enhance RORγt transcription factor activity. This study thus demonstrates that sumoylation is a critical mechanism for regulating RORγt function, and reveals new drug targets for preventing TH17-mediated autoimmunity.
The transcription factor RORγt directs the differentiation of TH17 cells, which secrete IL-17 and participate in both protective and pathological immunity1. The clearance of pathogens such as Citrobacter rodentium and fungus depends on robust protective TH17 immunity2,3,4,5,6. On the other hand, TH17 cells also mediate the pathological immune responses involved in autoimmune conditions, such as multiple sclerosis, colitis, and even autism, and the prevention of these conditions depends on inhibiting the formation and function of TH17 cells7,8,9,10,11,12. The critical function of RORγt has been demonstrated by severe immune deficiency in both mice13 and humans14 carrying mutated versions of the RORγt-encoding gene Rorc. In addition, RORγt enhances thymocyte survival and is thus essential for thymic T cell development. RORγt is also required for the biogenesis of secondary lymph tissues, including gut-associated Peyer’s patches15,16,17,18. Because RORγt is required for the generation of pathogenic TH17 cells responsible for autoimmunity, it is an attractive target for the development of drugs to control TH17-mediated immunological disorders19,20. It is thus important to understand the mechanisms regulating RORγt function.
As a member of the steroid nuclear receptor superfamily, RORγt has two conserved domains21,22: an amino-terminal DNA-binding domain and a carboxyl-terminal ligand-binding domain. The very carboxyl terminal of the ligand-binding domain is an activation function 2 (AF2) motif responsible for recruiting steroid receptor coactivator 1 (SRC1) to nuclear receptors, which is required for RORγt-mediated transactivation of genes essential for TH17 differentiation23,24,25. Because RORγt is a transcription factor, previous studies have focused on the transcriptional aspects of RORγt function. However, the post-translational mechanisms that regulate RORγt function have long been neglected.
Sumoylation is a type of post-translational modification in which small ubiquitin-related modifier (SUMO) proteins are covalently attached to the lysines of target proteins. Mammals usually express three SUMO proteins: SUMO1, SUMO2, and SUMO3, which share approximately 50% amino acid sequence identity. Sumoylation is a multi-step reaction that is sequentially catalyzed by a SUMO-activating E1 enzyme, the single conjugating E2 enzyme Ubc9, and an E3 ligase. Sumoylation controls many aspects of cellular function26,27 by regulating protein stability and by enabling new protein–protein interactions through the addition of new docking sites. Knockout of the E2 enzyme Ubc9 affects thymic T cell development and the expansion of regulatory T cells28,29, implicating sumoylation as an important regulator of these two processes. However, the roles of sumoylation in other aspects of T cell development and function, including TH17 differentiation, remain unknown.
Here, we demonstrate that the loss of Sumo3, but not Sumo1, impairs TH17 differentiation and delays progression of thymic immature single-positive (ISP) CD8+ cells, which are similar to phenotypes observed in Rorγt−/− mice. This work leads us to identify lysine 31 (K31) as a functional sumoylation site in RORγt. We find that mice expressing K31-mutant RORγtK31R are incapable of being sumoylated at K31 and exhibit multiple defective RORγt-dependent functions, including differentiation of TH17, induction of TH17-dependent experimental autoimmune encephalomyelitis (EAE), the progression of thymic ISP, and development of Peyer’s patches. Additional data attribute these effects to the defective recruitment of histone acetyltransferase KAT2A, which impairs the interactions between RORγt and co-activator SRC1. Finally, we identify the E3 ligase responsible for RORγt sumoylation to be PIAS (protein inhibitor of activated STAT) proteins form the largest family of sumoylation E3 ligases30, as the E3 ligase PIAS4 is able to bind and sumoylate RORγt at K31, and knockdown of PIAS4 impairs RORγt-dependent TH17 differentiation and progression of ISP, phenocopying the effects observed in RORγtK31R/K31R mice. Our study thus reveals sumoylation as a novel post-translational mechanism for regulating RORγt-dependent functions.
Sumo3, but not Sumo1, stimulates TH17 differentiation
To investigate whether sumoylation plays a role in T helper cell differentiation, we examined the differentiation of Sumo1−/− and Sumo3-−/− CD4+ T lymphocytes (Sumo2−/− mice are embryonic lethal31). Deletion of Sumo1 compromised TH1 and Treg differentiation, but did not affect TH2 differentiation (Supplementary Fig. 1a). Deletion of Sumo3, but not Sumo1, dramatically impaired TH17 differentiation (Fig. 1a) and decreased expression of critical TH17 genes (Fig. 1b). However, Sumo3−/− CD4+ T cells could normally differentiate into TH1, TH2, and Treg lineages (Supplementary Fig. 1b). We next adoptively transferred Sumo3−/− CD4+ T cells into Rag1−/− mice to test their ability to induce EAE. Rag1−/− mice reconstituted with Sumo3−/− CD4+ T cells had attenuated disease severity (Fig. 1c), which correlated with lower infiltration of lymphocytes, including Ly6G+ neutrophils, CD4+ T cells, and CD11b+Ly6C+ monocytes, into the central nervous system (CNS; Fig. 1d and Supplementary Fig.1c for gating strategy). In addition, the percentages (Supplementary Fig.1d) and numbers (Fig. 1e) of CNS-infiltrating IL-17A+, IFNγ+, GM-CSF+, IL-17A+IFNγ+, and IL-17A+GM-CSF+ CD4+ T cells responsible for EAE were also significantly lower in these mice7,8,9. These results suggest that SUMO3, but not SUMO1, promotes RORγt-dependent TH17 differentiation.
Sumo3, but not Sumo1, is required for thymic ISP progression
To determine whether sumoylation plays a role in RORγt-dependent thymocyte development, we analyzed thymocytes from Sumo3−/− and Sumo1−/− mice. The thymic cellularity of Sumo1−/− (Fig. 2a) and Sumo3−/− (Fig. 2b) mice was equivalent to that of wild-type (WT) mice. We then analyzed CD4 and CD8 markers to monitor the three sequential stages of thymocyte development: CD4−CD8− double-negative (DN), CD4+CD8+ double-positive (DP), and CD4+ or CD8+ single-positive (SP). We did not detect obvious differences in the overall percentages of DN, DP, and SP populations in the thymi of WT versus Sumo1−/− mice (Fig. 2c). We did, however, notice an increased percentage of CD8+ SP cells in the thymi of Sumo3−/− mice (Fig. 2d). Further scrutiny of the CD8+ SP population revealed a significantly greater percentage of immature TCRloCD24hiCD8+ cells (ISPs), as well as a correspondingly lower percentage of mature TCRhiCD24loCD8+ cells, in the thymi of Sumo3−/− (Fig. 2e), but not Sumo1−/− (Fig. 2f), mice. These findings indicate the selective function of Sumo3 in the progression of ISP, which is RORγt-dependent18. Furthermore, whereas the absolute number of ISPs was increased in Sumo3−/− compared to WT thymi (Supplementary Fig. 1e), there was no difference in the number of mature TCRhiCD24loCD8+ cells in WT and Sumo3−/− thymi, suggesting that the overall increase in CD8+ SP cells observed in Sumo3−/− thymi is due to increased ISPs and not mature CD8+ cells. To determine the intrinsic function of SUMO3 in thymocyte development, we isolated and co-cultured CD4−CD8− DN thymocytes with OP9-DL4 stroma cells to observe their differentiation in vitro32 (Fig. 2g). Although both WT and Sumo3−/− DN cells could differentiate into DP and SP populations, there were increased percentages and numbers of CD8+ SP but not CD4+ SP cells in Sumo3−/− cultures (Fig. 2g, top panels). Furthermore, we detected significantly more TCRloCD24hiCD8+ ISPs among Sumo3−/− CD8+ cells than among WT CD8+ cells (Fig. 2g, bottom panels), suggesting the intrinsic requirement of SUMO3 for the progression of ISPs. We previously found that, similarly to the deletion of Sumo3 shown here, the deletion of RORγt in mice resulted in more ISPs and reduced TH17 differentiation33, which suggested that RORγt may be SUMO3-modified.
Sumoylation of K31 is essential for RORγt function
To determine whether RORγt is sumoylated, we monitored the sumoylation of immunoprecipitated RORγt using anti-SUMO1 and anti-SUMO3 antibodies (Fig. 3a, and Supplementary Fig 9 for the full-length image of immunoblot). Whereas SUMO1-modified RORγt (SUMO1-RORγt) was barely detectable (Fig. 3a, top panel), SUMO3-modified RORγt (SUMO3-RORγt) produced strong signals in both TH17 cells and thymocytes (Fig. 3a, second panel). To identify the sumoylated residues, immunoprecipitated RORγt was subject to mass spectrometric analysis to detect a signature peptide containing a “QTGG” remnant at the sumoylation site. Lysines 11 and 31 (K11 and K31) were identified as the sumoylation sites in RORγt (Supplementary Fig. 2a). To confirm these sites, K11 and K31 were mutated to arginine to prevent the sumoylation, and sumoylation of purified WT and mutant RORγt was compared in vitro (Fig. 3b). As expected, we observed that SUMO3-RORγt could not be detected in the absence of the E2 enzyme Ubc9 or SUMO3. We also observed that, whereas the K11R mutation (RORγtK11R) did not affect SUMO3-RORγt levels, the K31R mutation (RORγtK11R) greatly reduced SUMO3-RORγt (Fig. 3b). In contrast, we could not detect obvious SUMO1-modified RORγt, RORγtK11R, or RORγtK31R (Fig. 3c). These results strongly suggest that RORγt is SUMO3- but not SUMO1-modified at K31. Interestingly, sequence alignment indicated that K31 and its surrounding amino acid sequence are highly conserved in RORγt across species (Supplementary Fig. 2b), suggesting the importance of K31 as a sumoylation site.
To determine the role of K31 sumoylation in RORγt-dependent functions, we compared the ability of retrovirally expressed RORγt and RORγtK31R to rescue TH17 differentiation in Rorγt−/− CD4+ T cells (Fig. 3d). As expected, Rorγt−/− CD4+ T cells transduced with retroviruses expressing GFP alone (empty virus, EV) failed to differentiate into TH17 cells. TH17 cell differentiation was rescued by WT RORγt and RORγtK11R, but not RORγtK31R (Fig. 3d, and Supplementary Fig. 2c for gating strategy), although both mutants were expressed at the levels comparable to WT RORγt expression (Fig. 3e). Consistent with these results, the expression of critical TH17 genes was lower in RORγtK31R-reconstituted Rorγt−/− T cells than in WT RORγt-reconstituted T cells (Fig. 3f), confirming that the TH17 differentiation program is impaired when K31 sumoylation is blocked.
To determine whether K31 sumoylation is essential for RORγt-regulated thymocyte development, we compared the development of Rorγt−/− thymocytes retrovirally reconstituted with RORγt, RORγtK11R, and RORγtK31R in vitro (Fig. 3g, and Supplementary Fig. 2d for gating strategy). Isolated Rorγt−/− CD4−CD8− DN thymocytes transduced with retroviruses simultaneously expressing GFP and RORγt or RORγtK11R, but not expressing GFP alone (EV), differentiated into CD4+CD8+ DP and CD4+ SP cells. However, retroviral expression of RORγtK31R failed to fully restore thymocyte development, indicated by more CD4−CD8− DN and CD8+ SP cells and fewer CD4+CD8+ DP and CD4+ SP cells (Fig. 3g). Interestingly, the expression of surface CD4, which is lower in Rorγt−/− thymocytes than in WT thymocytes18, was rescued in Rorγt−/− cells reconstituted with WT RORγt or RORγtK11R but not with RORγtK31R (Fig. 3h), suggesting a role of K31 sumoylation in the regulation of CD4 expression. Altogether, these data demonstrate that blocking sumoylation at K31 impairs RORγt functions in thymocyte development and TH17 differentiation in vitro.
RORγt K31R/K31R mice exhibit defective TH17 differentiation
To investigate the function of K31 sumoylation in vivo, we generated a strain of mouse expressing RORγtK31R (RORγtK31R/K31R) (Supplementary Fig. 3a–3c). The number of splenocytes was slightly higher in RORγtK31R/K31R mice than in WT mice, which was partially attributed to increased CD8+ but not CD4+ T cells (Supplementary Fig. 4a). T cells from RORγtK31R/K31R mice consistently exhibited defective TH17 differentiation, as indicated by the lower generation of IL-17A+ cells compared to WT mice (Fig. 4a) and decreased expression of critical TH17 genes (Supplementary Fig. 4b). However, the T cells from RORγtK31R/K31R mice differentiated into TH1, TH2, and Treg comparably to T cells from WT mice (Supplementary Fig. 4c), suggesting a selective defect in differentiation into TH17 cells. The observed reduction in TH17 differentiation was not due to decreased expression of RORγtK31R, which was comparable with WT RORγt expression in TH17 cells (Fig. 4b). To confirm that K31R mutation affects the sumoylation of RORγt in vivo, we compared levels of SUMO3- RORγt in differentiated TH17 cells from WT and RORγtK31R/K31R mice. Indeed, SUMO3-modified (Fig. 4c), but not ubiquitin-modified (Supplementary Fig. 4d), RORγt was significantly reduced in RORγtK31R TH17 cells, confirming that RORγt-K31 is sumoylated in vivo.
To assess the global effects of K31 sumoylation on TH17 differentiation, we mapped RORγt DNA-binding sites using ChIP-seq and gene expression profiles using RNA-seq in WT and RORγtK31R/K31R TH17 cells. We found similar expression patterns in biological replicates of WT and RORγtK31R/K31R TH17 cells, as indicated by the heat map in Fig. 4d showing similarly up and downregulated genes. Many TH17 genes, including Il17a, Il17f, Ccl20, Csf2, Ccr6, and Il1r1, but not Rorc, were down-regulated in RORγtK31R/K31R TH17 cells (Fig. 4e), suggesting an essential function of RORγt K31 sumoylation in the expression of genes critical for TH17 differentiation. ChIP-seq analysis identified DNA-binding peaks within critical TH17 gene loci, including Il17a and Il17f, which overlap well with our previously identified RORγt DNA-binding peaks (Supplementary Fig. 4e)33. Furthermore, we conducted a search among all the RORγt DNA-binding peaks to identify potential transcription factor binding motifs, and the most enriched motif was the RORγt binding site in both WT and RORγtK31R/K31R TH17 cells (Supplementary Fig. 4f), validating the results of our ChIP-seq assay. We more carefully compared the RORγt DNA-binding peaks at the IL-17 loci in WT and RORγtK31R/K31R TH17 cells (Fig. 4f, top two panels), using the Hbb locus as a negative control (Fig. 4f, bottom panel). Some RORγtK31R DNA-binding peaks were smaller than those of WT RORγt at the Il17a locus (Fig. 4f), indicating that reduced RORγtK31R DNA-binding affinity likely contributes to the reduced expression of Il17a observed in RORγtK31R TH17 cells. On the other hand, the RORγtK31R DNA-binding peaks at the IL17f locus were just as great as, if not greater than, those of WT RORγt, which suggests that sumoylation of RORγt may stimulate the expression of Il17f via DNA-binding-independent mechanisms. These findings were further confirmed using individual ChIP assays (Fig. 4g). Taken together, our results demonstrate that K31 sumoylation of RORγt promotes TH17 differentiation by activating the expression of the critical TH17 genes.
RORγt K31R/K31R mice are resistant to induction of EAE
To determine the function of sumoylation in RORγt-dependent immunity in vivo, we induced EAE in WT and RORγtK31R/K31R mice. The severity of the disease was markedly attenuated in RORγtK31R/K31R mice compared with WT mice (Fig. 5a), which was reflected in lower CNS infiltration by various mononuclear cells (Fig. 5b), indicating reduced inflammation. In addition, there was lower expression of critical TH17 genes in CNS-infiltrating lymphocytes recovered from RORγtK31R/K31R mice than in those recovered from WT mice (Fig. 5c). Therefore, we have demonstrated that sumoylation of RORγt-K31 modulates TH17-mediated EAE in vivo.
Because both TH17 and TH1 cells can induce EAE34, we compared the ability of TH1 and TH17 cells derived from RORγtK31R/K31R and WT mice to induce passive EAE. For this purpose, T cells from WT or RORγtK31R/K31R mice primed with myelin oligodendrocyte glycoprotein 35–55 (MOG35–55) were cultured and stimulated with MOG35–55 in vitro under TH17- or TH1-polarizing conditions, and then adoptively transferred to Rag1−/− mice to induce EAE. Compared to their WT counterparts, RORγtK31R/K31R T cells that were expanded under TH17 conditions induced less severe EAE (Fig. 5d), which was associated with lower CNS infiltration by various mononuclear lymphocytes (Supplementary Fig. 5a) and lower expression of critical TH17 genes in lymphocytes recovered from the CNS (Supplementary Fig. 5b). In contrast, RORγtK31R/K31R and WT T cells stimulated under TH1 conditions did not differentially induce EAE in Rag1−/− mice (Fig. 5e), which was demonstrated by mostly comparable numbers of various CNS-infiltrating mononuclear lymphocytes (Supplementary Fig. 5c). These results indicate that sumoylation at K31 is required selectively for RORγt-dependent TH17 immunity in vivo.
ISPs accumulate in thymi of RORγt K31R/K31R mice
To determine the function of K31 RORγt sumoylation in thymocyte development, we analyzed thymocytes from WT, RORγt−/−, and RORγtK31R/K31R mice. The expression of RORγtK31R and WT RORγt was equivalent in CD4+CD8+ thymocytes (Fig. 6a, top panel) and non-detectable in CD4+ SP cells (Fig.6a, bottom panel), suggesting that K31R does not disturb the expression pattern of RORγt. However, SUMO3-modified (Supplementary Fig. 6a), but not ubiquitinated (Supplementary 6b), RORγt was lower in RORγtK31R/K31R thymocytes compared to those of WT thymocytes, confirming K31 as an RORγt sumoylation site in thymocytes. RORγt is known to regulate the survival and cell cycle of thymocytes by upregulating the expression of Bcl-xL18. However, WT and RORγtK31R/K31R mice had comparable thymocyte survival (Supplementary Fig. 6c) and percentages of cells with > 2 N of DNA (in the DNA synthesis phase) (Supplementary Fig. 6d). In addition, RORγtK31R/K31R mice had lower rather than greater thymic cellularity that WT mice (Fig. 6b). These results suggest that K31 sumoylation is dispensable for RORγt-dependent thymocyte survival and cell cycle regulation. Analysis of the surface markers CD4 and CD8 revealed greater percentages of CD4−CD8− DN and CD8+ SP cells in RORγtK31R/K31R thymi than in WT thymi, similar to those observed in Rorγt−/− thymi (Fig. 6c, three panels on the left, and Supplementary Fig. 6e). In addition, the absolute number of CD8+ SP but not CD4+ SP cells (Fig. 6c, two panels on right) was greater in RORγtK31R/K31R thymi than in WT. Among CD8+ SP cells, there was a higher frequency of immature TCRloCD24hiCD8+ cells (ISPs) in RORγtK31R/K31R thymi, similar to the frequency in Rorγt−/− thymi (Fig. 6d), as well as Sumo3−/− thymi (Fig. 2e). Not only the frequency but the absolute number of ISPs was greater in RORγtK31R/K31R thymi compared to WT thymi, whereas the cellularity of mature TCRhiCD24loCD8+ cells was comparable between groups (Supplementary Fig. 6f). These results indicate the critical function of RORγt-K31 sumoylation in the progression of ISPs. In addition, we observed lower levels of surface CD4 on CD4+CD8+ DP cells in both RORγtK31R/K31R and Rorγt−/− thymi compared to WT thymi (Fig. 6e), suggesting a positive role of RORγt-K31 sumoylation in CD4 expression. We next compared the differentiation of sorted WT and RORγtK31R/K31R CD4−CD8− DN thymic cells co-cultured with stroma cells in vitro (Fig. 6f). As expected, DN cells derived from RORγtK31R/K31R mice gave rise to a higher frequency of CD8+ SP cells and a greater percentage of TCRloCD24hiCD8+ ISPs than DN cells from WT mice. We thus separated the functions of RORγt in thymocyte development into two categories: (1) K31 sumoylation-independent functions, including survival and cell cycle, and 2) K31 sumoylation-dependent functions, including the progression of ISPs and CD4 expression.
To better understand the K31 sumoylation-dependent and -independent functions of RORγt, we mapped the landscape of RORγt DNA-binding sites and examined the gene expression profiles of WT and RORγtK31R/K31R thymocytes using ChIP-seq and RNA-seq assays. We observed similar expression patterns among biological replicates of WT or RORγtK31R/K31R thymocytes, as suggested by the color patterns in the clustered heat map shown in Fig. 6g, which demonstrates the reproducibility of our RNA-seq assay. We previously showed that the reduced survival and dysregulated cell cycle of Rorγt−/− thymocytes were associated with significant changes in the expression of several important cell survival and cell cycle regulators33. The expression of several of these regulators, Pik3r3, Wee1, Bcl2, Myc, and Bcl2l1, was not significantly different between WT and RORγtK31R/K31R thymocytes (Fig. 6h, genes listed on the left in orange). Therefore, K31 sumoylation does not appear to be required for the expression of these critical survival and cell cycle regulators, which explains why thymocyte survival and cell cycle regulation are K31 sumoylation-independent. However, we identified several genes, including Rasgrp1, Ets2, Cd6, Ptcra, Rasgrp4, and Cd4, that were downregulated in RORγtK31R/K31R thymocytes compared to WT thymocytes (Fig. 6h, genes listed on the right in red) and are known to regulate thymocyte development35,36,37,38. We found that the CD4−encoding Cd4 gene was downregulated in RORγtK31R/K31R thymocytes compared to WT thymocytes (Fig. 6h, top gene in red), which is consistent with lower protein levels of CD4 on RORγtK31R/K31R thymocytes (Fig. 6e), as well as Rorγt−/− thymocytes retrovirally reconstituted with RORγtK31R (Fig. 3h). In particular, Cd6 was reported to regulate the progression of ISPs35. Therefore, we have demonstrated that RORγt K31 sumoylation is required for the transactivation of these genes, which are likely responsible for K31 sumoylation-dependent functions, such as the progression of ISPs. Our ChIP-seq assay also identified obvious RORγt DNA-binding peaks at Bcl2l1(Fig. 6i, j), Cd6, Ets2, Rasgrp1, and Cd4 loci (Supplementary Fig. 6g), suggesting that they are direct targets of RORγt. Interestingly, RORγtK31R binds to the same sites on these gene loci as WT RORγt, suggesting that K31 sumoylation is not required for the DNA binding of RORγt. Therefore, K31 sumoylation of RORγt likely regulates the expression of these target genes through DNA binding-independent mechanisms.
RORγt is required for the development of secondary lymph tissues18. To determine the roles of K31 sumoylation in RORγt-dependent organogenesis, we examined lymph tissues in RORγtK31R/K31R and WT mice. RORγtK31R/K31R mice had all the lymph nodes observed in WT mice except for Peyer’s patches (Supplementary Fig. 6h), suggesting a selective role of K31 sumoylation in the biogenesis of Peyer’s patches.
Sumoylation stabilizes RORγt–KAT2A–SRC1 complexes
One function of sumoylation is to regulate protein stability39. However, this does not seem to be the case for K31 sumoylation of RORγt, as the protein levels of RORγt and RORγtK31R in both TH17 cells (Fig. 4b) and thymocytes (Fig. 6a) were equivalent and the degradation rates of RORγt and RORγtK31R were comparable (Supplementary Fig. 7a). Given that sumoylation can also regulate protein–protein interactions by adding a new docking site, we tested whether K31 sumoylation regulated the binding of RORγt to its co-factors. RORγt is known to interact with co-activator SRC1 to regulate TH17 differentiation25. Indeed, we found that RORγtK31R, compared to WT RORγt and the controls RORγtK11R and RORγtK69R, had impaired interactions with SRC1 (Fig. 7a). This finding was supported by the lower detection of endogenous RORγtK31R-SRC1 complexes in RORγtK31R/K31R TH17 cells (Fig. 7b, left panel) and thymocytes (Fig. 7b, right panel) compared to their WT counterparts. Furthermore, our ChIP-seq assay showed that the recruitment of SRC1 to the Il17a and Il17f loci by RORγtK31R was much less than that by WT RORγt in TH17 cells (Fig. 7c), again confirming reduced RORγtK31R-SRC1 interactions compared to RORγt-SRC1 interactions.
We found in the literature a report that KAT2A (or GCN5), a histone acetyltransferase, is able to synergize with SRC1 to bind to nuclear receptors40. Furthermore, using mass spectrometry, we identified with high confidence that KAT2A and SRC1 are RORγt-binding proteins in both thymocytes and TH17 cells (Supplementary Fig. 7b). Indeed, in HEK293T cells, we detected the RORγt–KAT2A interaction, which was further enhanced by the addition of SUMO3 and Ubc9. In contrast, the RORγtK31R–KAT2A interaction was much weaker and was not affected by SUMO3 and Ubc9 (Fig. 7d). These results suggest that the sumoylation of K31 promotes the interaction between RORγt and KAT2A. Furthermore, we found that the expression of KAT2A enhanced the RORγt–SRC1 interaction in HEK293T cells (Fig. 7e). On the other hand, knockdown of endogenous KAT2A greatly impaired the RORγt-SRC1 interaction in TH17 cells (Fig. 7f), suggesting that KAT2A promotes the RORγt–SRC1 interaction. In addition, immunoprecipitation of RORγtK31R brought down much less KAT2A and SRC1 compared to immunoprecipitation of WT RORγt in both TH17 cells (Fig. 7b, left panel) and thymocytes (Fig. 7b, right panel), suggesting an essential role of K31 sumoylation in the formation of stable RORγt–KAT2A–SRC1 complexes. RORγt and SRC1 have already been established as essential for TH17 differentiation13,25, we thus aimed to determine the function of KAT2A in TH17 differentiation using a knockdown approach. We found that the knockdown of KAT2A (Supplementary Fig. 7c) impaired TH17 differentiation (Fig. 7g) and decreased expression of critical TH17 genes (Supplementary Fig. 7d). Of the two short hairpin RNAs used into knockdown KAT2A (shKAT2A-1 and shKAT2A-2), shKAT2A-2 inhibited TH17 differentiation more potently, which correlated with its higher potency in repressing KAT2A expression (Supplementary Fig. 7c), demonstrating an essential role of KAT2A in TH17 differentiation. Taken together, these data show that K31 sumoylation promotes the recruitment of KAT2A and SRC1 to RORγt to drive TH17 differentiation.
PIAS4 catalyzes the K31 sumoylation of RORγt
PIAS proteins form the largest family of sumoylating E3 ligases30. To identify the E3 responsible for RORγt sumoylation, we first monitored the interactions between RORγt and individual PIAS proteins (Fig. 8a). We could not detect interactions between RORγt and PIAS2 or PIAS3. However, we detected a weak PIAS1–RORγt interaction and a strong PIAS4-RORγt interaction. We next sought to determine whether PIAS1 and PIAS4 could sumoylate RORγt at K31. For this purpose, we mutated all lysines of RORγt except K31 to arginines (RORγt-K31) so that only K31 could be sumoylated. Whereas we detected a relatively low amount of SUMO3-modified RORγt-K31 in the presence of PIAS1, we detected a much stronger SUMO3-modified RORγt-K31 signal in the presence of PIAS4 (Fig. 8b). As expected, we could barely detected any SUMO1-modified RORγt-K31 in the presence of PIAS1 or PIAS4 (Supplementary Fig. 8a), suggesting that PIAS4 and to a lesser extent PIAS1 can catalyze the addition of SUMO3, but not SUMO1, to K31 of RORγt.
To further evaluate the role of PIAS4 in regulating RORγt-dependent functions, we assessed the effects of PIAS4 knockdown on TH17 differentiation and thymocyte development in vitro. Knockdown of PIAS4 (Supplementary Fig. 8b) resulted in impaired TH17 differentiation (Fig. 8c) and reduced expression of important TH17 signature genes (Fig. 8d). However, knockdown of PIAS1 (Supplementary Fig. 8b) did not affect TH17 differentiation, which is consistent with a report that PIAS1 is not required for TH17 differentiation41. We next monitored the in vitro differentiation of sorted CD4-CD8− thymocytes when PIAS1 or PIAS4 was knocked down. Knockdown of PIAS4 led to a slightly increased percentage of CD8+ SP cells (Fig. 8e, top panels). More importantly, there was a dramatically greater percentage of TCRloCD24hi CD8+ ISPs, and a correspondingly lower percentage of TCRβloCD24hi CD8+ cells, among CD8+ SP cells in the cells with PIAS4 knocked down (Fig. 8e, bottom panels). In contrast, the knockdown of PIAS1 had no effect on the percentage of TCRβloCD24hi CD8+ ISPs (Supplementary Fig. 8d). Therefore, the knockdown of PIAS4 impaired TH17 differentiation and increased ISPs, which were both phenotypes observed in Sumo3−/− and RORγtK31R/K31R mice. Taken together, these data suggest that PIAS4 catalyzes the addition of SUMO3 to K31 of RORγt and thus regulates RORγt-dependent TH17 differentiation and progression of ISPs.
RORγt controls the function of TH17 cells, which mediate both protective and pathogenic immunity. However, little is known about the post-translational mechanisms that regulate RORγt function. Our in vitro and in vivo results demonstrate that sumoylation of RORγt is a novel regulatory mechanism for controlling RORγt-dependent TH17 immunity, thymic ISP progression, and development of Peyer’s patches: (1) Sumo3−/− but not Sumo1−/− mice display defects in RORγt-regulated TH17 differentiation and thymocyte development (specifically, they accumulate ISPs); (2) RORγt is SUMO3- but not SUMO1-modified at K31 in both TH17 cells and thymocytes; (3) mice expressing RORγtK31R exhibit multiple defective RORγt-dependent functions, including differentiation of TH17 cells, induction of TH17-mediated EAE, progression of ISPs in the thymus, and development of Peyer’s patches. We also identified PIAS4 as the E3 that catalyzes K31 sumoylation and regulates TH17 differentiation and progression of thymic ISPs. Therefore, we have demonstrated that post-translational sumoylation is a novel mechanism for modulating RORγt-dependent TH17 immunity that can be targeted by clinical therapies to enhance protective and inhibit pathogenic TH17 immunity.
Previous studies have reported that RORγt function is regulated by ubiquitination, which is a post-translational modification similar to but distinct from sumoylation42,43. The ubiquitin E3 ligase, Itch, was found to bind and ubiquitinate RORγt for degradation and thus regulate TH17-dependent immune responses43, which explains why Itch−/− mice develop colitis. Another E3 ligase, UBR5, was also reported to regulate RORγt stability through the ubiquitin pathway42. However, the RORγt ubiquitination sites involved in the above two studies remain unknown. Meanwhile, we identified lysines 446 and 69 as ubiquitination sites through which RORγt-dependent TH17 differentiation can be controlled via degradation-independent mechanisms33,44. Therefore, we and others have demonstrated that TH17 immunity can be controlled through the ubiquitin pathway, which regulates RORγt stability and protein interactions. Although sumoylation can also regulate protein stability, our results do not support that K31 sumoylation affects RORγt stability. We showed that K31 sumoylation stimulates the recruitment of histone acetyltransferase KAT2A and co-activator SRC1 to RORγt. In addition, we showed that preventing K31 sumoylation reduces recruitment of SRC1 to the Il17f locus, suggesting that K31 sumoylation regulates the interaction between RORγt and its co-factors to activate Il17f expression.
RORγt has long been known to regulate thymocyte development18. However, RORγt chromatin occupancy and target genes in thymocytes were not known, which limited understanding of the mechanisms responsible for RORγt-regulated thymocyte development. To address this need, we mapped genome-wide RORγt DNA-binding sites and identified RORγt target genes. Furthermore, we identified K31 as the sumoylation site of RORγt, which enabled us for the first time to dissect RORγt functions in thymus. One important function of RORγt is to regulate thymocyte survival by up-regulating anti-apoptotic Bcl-xL expression18. Our results showed that K31 sumoylation is actually not required to up-regulate Bcl-xL or to maintain thymocyte survival; however, it is specifically required for the progression of thymic ISPs. Our study thus separates RORγt functions and establishes a link between RORγt-regulated functions and RORγt target genes.
TH17 cells produce the effector cytokines IL-17A, IL-17F, IL-22, and GM-CSF to mediate pathological inflammation responsible for many types of autoimmune diseases; targeting TH17 cells is thus a potential treatment for these diseases45. Indeed, inhibiting the TH17 pathway is effective for treating psoriasis and multiple sclerosis46,47. Considering the essential function of RORγt in TH17 cells, pharmaceutical and academic scientists are developing RORγt inhibitors to treat TH17-dependent autoimmunity11,19,20,48,49. Unfortunately, such RORγt inhibitors can induce thymic lymphoma by inhibiting RORγt during thymocyte development50. Although K31 sumoylation is required for the progression of thymic ISP, it is not essential for regulating thymocyte survival or cell cycle progression, which are most likely responsible for the development of lymphoma observed in RORγt−/− mice50,51. Therefore, we expect that drugs targeting the K31 sumoylation pathway will inhibit TH17-mediated pathological immunity without interfering with thymocyte survival or cell cycle regulation, which could induce lymphoma in patients. Therefore, in addition to revealing a novel post-translational modification-based mechanism for regulating RORγt-dependent T cell function, our results also facilitate the development of a new category of RORγt-based drugs to treat TH17-mediated autoimmunity without serious side effects.
Both the targeting vector and the knock-in RORγtK31R/K31R mice were designed and generated by Biocytogen LLC. RORγtK31R/K31R mice are available at The Jackson Laboratory as Stock No. 032604. Rag1−/− (002216) mice were purchased from the Jackson Laboratory. The Rorγt−/− (RORC2−/−)18, Sumo1−/−52, and Sumo3−/− mice31 were bred and housed under specific pathogen-free (SPF) conditions in the Animal Resource Center at the Beckman Research Institute of City of Hope under protocols approved by the Institutional Animal Care and Use Committee. Mice were 10–12 weeks of age for EAE studies and 6–8 weeks of age for all other experiments, with littermates age-matched across experimental groups.
Antibodies and cytokines
Antibodies against RORγt (Q31-378, BD Bioscience, dilution ratio 1:1000), SRC1 (128E7, Cell Signaling, dilution ratio 1:1000), β-actin (SC-8422, Santa Cruz Biotechnology, dilution ratio 1:1000), GFP (A11122, Life technology, dilution ratio 1:1000), KAT2A (ab18381, Abcam, dilution ratio 1:1000), HA (HA-7, Sigma-Aldrich, dilution ratio 1:1000), FLAG (M2, Sigma-Aldrich, dilution ratio 1:5000), SUMO1 (C9H1, Cell Signaling Tech, dilution ratio 1:1000), SUMO3 (ab34661, Abcam, dilution ratio 1:1000), and PIAS4 (AV33011, Sigma-Aldrich, dilution ratio 1:1000) were used for immunoblot analysis. Phycoerythrin (PE)-indotricarbocyanine (Cy7)-conjugated anti-CD8 (53-6.7, dilution ratio 1:200), PE-conjugated anti-RORγt (B2D, dilution ratio 1:100), allophycocyanin (APC)-conjugated anti-IL-17A (eBio17B7, dilution ratio 1:100), PE-conjugated anti-Thy1.2 (53-2.1, dilution ratio 1:200), PE-conjugated anti-CD24 (M1/69, dilution ratio 1:100), PE-conjugated anti-TCRβ (H57-597, dilution ratio 1:100), PE-indodicarbocyanine (Cy5)-conjugated anti-CD19 (eBio1D3, dilution ratio 1:100), PE-conjugated anti-CD11b (M1/70, dilution ratio 1:100), fluorescein isothiocyanate (FITC)-conjugated anti-CD4 (GK1.5, dilution ratio 1:200), APC-conjugated anti-IL-4 (11B11, dilution ratio 1:100), and APC-conjugated anti-Foxp3 (FJK-16s, dilution ratio 1:100) antibodies were from eBioscience. Monoclonal antibodies against mouse CD3 (145-2C11), CD28 (37.51), IL-4 (11B11), IFN-γ (XMG1.2), and the p40 subunits of IL-12 and IL23 (C17.8), as well as PE-Cy7-conjugated anti-Ly6G (1A8, dilution ratio 1:100), FITC-conjugated anti-IFN-γ (XMG1.2, dilution ratio 1:100), PE-conjugated anti-GM-CSF (MP1-22E9, dilution ratio 1:100), FITC-Cy7-conjugated anti-CD45 (104, dilution ratio 1:200), and PE-conjugated anti-CD25 (PC61.5, dilution ratio 1:100) antibodies, were purchased from BioLegend. Goat anti-hamster antibody was from MP Biomedicals. APC-conjugated anti-CD3 (UCHT1, dilution ratio 1:200) and FITC-conjugated anti-CD44 (IM7, dilution ratio 1:100) antibodies were from BD Pharmingen. Recombinant mouse IL-12, IL-4, IL-6, IL-23, and TGFβ were from Miltenyi Biotech. Recombinant mouse IL-2 was from Pepro Tech. The antibody against RORγt used for ChIP was a generous gift from Dan Littman at New York University.
cDNA encoding RORγt or SRC1 was inserted into a XhoI/EcoRI-cut pMSCV vector44. Point mutations of RORγt were generated using a site-directed mutagenesis kit from Agilent Technologies. pRK5-HA-ubiquitin (a gift from Ted Dawson at Johns Hopkins University School of Medicine; #17603-17608), pCMV-sport2-mGCN5 (a gift from Sharon Dent at MD Anderson Cancer Center; #23098), and constructs for expressing FLAG-PIAS (gifts from Ke Shuai at the University of California Los Angeles; #15206-15210) were obtained from Addgene. pCMV-FLAG-SUMO1, pCMV-FLAG-SUMO3, and pcDNA-UBC9 were generous gifts from Yuan Chen at the City of Hope. The LMP vector-based retroviral short hairpin RNA (shRNA)-expressing vectors were constructed using following oligonucleotide sequences: shKAT2A-1: TGCTGTTGACAGTGAGCGACCGCTATCTGGGCTACATCAATAGTGAAGCCACAGATGTATTGATGTAGCCCAGATAGCGGCTGCCTACTGCCTCGGA; shKAT2A-2: TGCTGTTGACAGTGAGCGCGCCAAGAATGCCCAAGGAATATAGTGAAGCCACAGATGTATATTCCTTGGGCATTCTTGGCATGCCTACTGCCTCGGA; shPIAS1-1: TGCTGTTGACAGTGAGCGAGGAACTAAAGCAAATGGTTATTAGTGAAGCCACAGATGTAATAACCATTTGCTTTAGTTCCGTGCCTACTGCCTCGGA; shPIAS1-2: TGCTGTTGACAGTGAGCGCCCGGATCATTCTAGAGCTTTATAGTGAAGCCACAGATGTATAAAGCTCTAGAATGATCCGGATGCCTACTGCCTCGGA; shPIAS4−1: TGCTGTTGACAGTGAGCGCGCTACAGAGGTTGAAGACGATTAGTGAAGCCACAGATGTAATCGTCTTCAACCTCTGTAGCATGCCTACTGCCTCGGA; shPIAS4-2: TGCTGTTGACAGTGAGCGCGAGCTGTATGAGACTCGCTATTAGTGAAGCCACAGATGTAATAGCGAGTCTCATACAGCTCTTGCCTACTGCCTCGGA.
Platinum-E packaging cells (Cell Biolabs) were plated in a 10-cm dish in 10 ml RPMI-1640 medium plus 10% FBS. 24 h later, cells were transfected with empty pMSCV or pLMP vectors or the appropriate retroviral expression plasmids with BioT transfection reagent (Bioland). After overnight incubation, the medium was replaced and cultures were maintained for another 24 h. Viral supernatants were collected 48 and 72 h later, passed through 0.4-μm filters (Millipore), and supplemented with 8 µg/ml of polybrene (Sigma-Aldrich) and 100 U/ml of recombinant IL-2 (for transducing CD4+ T cells) or 5 ng/ml of recombinant IL-7 (for transducing CD4-CD8− thymocytes). Naïve CD4+ T cells were first activated with 0.25 µg/ml hamster anti-CD3 (145-2C11; Biolegend) and 1 µg/ml hamster anti-CD28 (37.51; Biolegend) in 24−well plates pre-coated with 0.2 mg/ml goat anti-hamster antibody for 24 h, then spin-infected with viral supernatants (1200 g, 30°C for 2 h). The retroviral supernatant was also used to infect CD4−CD8− thymocytes that had been co-cultured with feeder OP9-DL4 cells (a generous gift from Ellen Rothenberg at Caltech) in the presence of recombinant IL-7 (5 ng/ml) for 24 h. After spin infection, the viral supernatant was replaced with culture media containing polarizing cytokines for in vitro differentiation (for transduced CD+ T cells) or 5 ng/ml of recombinant IL-7 for in vitro T cell development (for transduced CD4−CD8− thymocytes), as described below.
In vitro differentiation
Naïve CD4+ T cells were purified from C57BL/6, RORγt−/−, or RORγtK31R/K31R mice by negative selection (Miltenyi Biotec). Suspensions of 4 × 105 cells/ml Iscove’s modified DMEM (Cellgro) containing 2 mM L-glutamine, 50 mM 2-ME, 100 U/ml penicillin, 100 mg/ml streptomycin, and 10% FBS were cultured in 24-well plates pre-coated with 0.2 mg/ml goat anti-hamster antibody for three days. The medium was supplemented with 0.25 µg/ml hamster anti-CD3, 1 µg/ml hamster anti-CD28, and polarizing cytokines: 2 ng/ml TGF-β, 20 ng/ml IL-6, 5 µg/ml anti-IL-4, and 5 µg/ml anti-IFNγ for TH17 differentiation; 20 µg/ml IL-12 and 5 µg/ml anti-IL-4 for Th1 differentiation; 10 ng/ml IL-4 and 10 µg/ml anti-IFNγ for TH2 differentiation; or 5 ng/ml TGF-β for Treg differentiation. For analysis, cells obtained from in vitro cultures were incubated for 4–5 h with 50 ng/ml PMA (Sigma-Aldrich), 750 ng/ml ionomycin (Sigma-Aldrich), and 10 µg/ml brefeldin A (BD Biosciences) in a tissue culture incubator at 37 °C, followed by intracellular cytokine staining.
In vitro T cell development
Thymocytes were stained with 7-AAD and antibodies against Thy1.2, CD4, and CD8. Specific 7-AAD−Thy1.2+CD4−CD8−populations were sorted using a FACSAria (BD Biosciences) and cultured at 5 × 105/ml overnight on an 80% confluent OP9-DL4 monolayer in flat-bottom 24-well culture plates with αMEM (MEM α medium; Invitrogen Life Technologies) supplemented with 20% FBS, 100 U/ml penicillin–streptomycin, 2 mM L-glutamine (Invitrogen Life Technologies), and 5 ng/ml recombinant murine IL-7. After 72 h, co-cultures were harvested for flow cytometry analysis.
Mouse thymi or spleens were homogenized by crushing with the head of a 1-ml syringe in a petri dish, followed by straining through a 40-μm nylon filter. Red Blood Cell Lysing buffer (Sigma-Aldrich) was used for red cell lysis. Cells isolated from thymi or spleens, co-cultures harvested from in vitro development, and CD4+ T cells stimulated appropriately were stained for surface markers. Intracellular cytokines were stained with Fixation/Permeabilization solution (BD Cytofix/Cytoperm Kit; BD Biosciences). The expression of surface and intracellular markers were analyzed with FACSCanto (BD).
RNA sequencing and analysis
To measure gene expression in the thymi of WT or RORγtK31R/K31R mice, two separate samples were collected on different days, and thymocytes from four (two male and two female) were pooled each day. To determine the gene expression profile of TH17 cells, naive CD4+ T cells were enriched from WT or RORγt K31R/K31R mice and polarized under TH17 conditions for three days. Cells were processed for RNA isolation (Qiagen). Quality verification, library preparation, and sequencing were performed at the City of Hope Integrative Genomics Core Facility. Eluted RNAs were prepared for sequencing using Illumina protocols and sequenced on an Illumina HiSeq 2500 to generate 51-bp reads. Sequenced reads were aligned to the mouse mm10 reference genome using TopHat. Gene expression levels were quantified using HTSeq, and edgeR was utilized to identify differentially expressed genes (fold-change > 1.5 and FDR < 0.05). Gene expression abundance was quantified as fragments per kilobase of transcript per million fragments mapped (FPKM). Heat maps of differentially expressed genes were made with gplots using log2-transformed FPKM values.
Chromatin immunoprecipitation and DNA sequencing (ChIP-seq)
A total of 2 × 107 cells were incubated with 1% formaldehyde to cross-link proteins with chromatin for 5 min at room temperature. 125 mM glycine was added to stop the cross-linking reaction. To shear genomic DNA into 200–500-bp fragments, cell lysates were sonicated using a water-bath sonicator (Covaris S200). Cell lysates were centrifuged (12,000 × g, 10 min) and incubated with specific antibodies (anti-RORγt from D. Littman or anti-SRC1 from Abcam) or IgG controls and protein A/G beads (Millipore). After extensive washing, DNA was eluted followed by reversion of the protein–DNA cross-linking. DNA was recovered for sequencing or qRT-PCR to quantify specific DNA fragments that were precipitated. Primers used for qRT-PCR are listed in Supplementary Table 1. Two biological replicates for each condition were sequenced on an Illumina HiSeq 2500 to produce 51-bp reads. Reads were aligned to the mm10 mouse genome using NovoAlign (http://www.novocraft.com/). TDF files were generated for visualization on the Integrative Genomics Viewer53. The enrichment of RORγt binding sites across the genome was analyzed using MACS2 with ‘—nomodel—extsize 150’54. The irreproducible discovery rate (IDR) framework was utilized find reproducible peaks across replicates. Enriched known TF motifs in ChIP-seq peaks were identified by using HOMER (findMotifsGenome.pl)55.
Quantitative real-time PCR
qRT-PCR was performed using SsoFast EvaGreen Supermix (Bio-Rad) in a CFX96 Real-Time PCR Detection System (Bio-Rad), using the primers listed in Supplementary Table 1. The amplification efficiency of all primers was previously tested, and the optimized conditions were used for all qRT-PCR reactions. Expression was calculated using the ΔΔxp method normalized to β-actin, and all measurements were performed in triplicate.
Thymocytes were freshly isolated from WT, RORγt−/−, or RORγtK31R/K31R mice and cultured in RPMI 1640 medium supplemented with 10% FBS, 100 U/ml penicillin–streptomycin, and 2 mM L-glutamine at 1 × 106 cells/ml. Thymocytes were incubated at 37 °C with 5% CO2. Dead cells were detected using Annexin V-PE and 7-AAD staining (BD Bioscience).
Induction and assessment of EAE
Active EAE was induced using an EAE induction kit, according to the manufacturer’s instructions (Hooke Laboratories, Lawrence, MA). Briefly, mice were subcutaneously immunized with a 200-ml myelin oligodendrocyte glycoprotein 35–55 (MOG35–55) peptide emulsion. On days 0 and 1 after immunization, mice were injected intraperitoneally with 200 ng Bordetella pertussis toxin. For TH17- or TH1-induced passive EAE, donor mice were immunized with MOG35–55 subcutaneously. 10 days later, cells were isolated from the spleen and lymph nodes and cultured with 20 µg/ml MOG35–55 for 3 days under either TH17-polarizing conditions (20 ng/ml rmIL23) or TH1-polarizing conditions (20 ng/ml rmIL-12; 2 µg/ml α-IL23p19). Rag1−/− recipient mice were then intraperitoneally transferred 3.0 × 107 MOG35–55-specific TH17 or TH1 cells. The severity of EAE was monitored and evaluated on a scale from 0 to 5 according to the Hooke Laboratories guidelines: 0 = no disease; 1 = paralyzed tail; 2 = hind limb weakness; 3 = hind limb paralysis; 4 = hind and forelimb paralysis; and 5 = moribund and death. When a mouse was euthanized because of severe paralysis, a score of 5 was entered for that mouse for the rest of the experiment.
Immunoprecipitation and immunoblot analysis
Cells were lysed in lysis buffer (1% Triton X-100, 20 mM Tris-cl, pH 7.4, 150 mM NaCl, and 5 mM EDTA) supplemented with protease inhibitor cocktail (Sigma) and 1 mM PMSF. Cell extracts were incubated overnight with 1 µg of the relevant antibodies, and proteins were immunoprecipitated for an additional 1 h at 4 °C with protein A/G-Sepharose beads (milipore). To detect sumoylation, transfected HEK293T cells, primary thymocytes, or polarized TH17 cells were lysed in lysis buffer containing 20 mM N-ethylmaleimide. Supernatant was supplemented with 1% SDS (vol/vol) and heated at 90 °C for 10 min. Samples were then diluted (1:10) with lysis buffer and incubated with anti-RORγt at 4 °C overnight. Enrichment of ubiquitinated proteins was performed as previously described44. Briefly, cell lysates were incubated with equilibrated Agarose-coupled Tandem Ubiquitin Binding Entity 1 (Agarose-TUBE1) (LifeSensors) at 4 °C for 4 h. After incubation, beads were washed four times with lysis buffer, resolved using SDS-PAGE, and analyzed using Western blot.
Prism software (GraphPad) was used for all statistical analyses. Two-tailed unpaired Student’s t-tests and one-way analysis of variance (ANOVA) were used to compare experimental groups. A P-value of less than 0.05 was considered statistically significant.
The data that support the findings of this study are available from the corresponding author upon request. The SRA (Sequence Read Archive) accession code for RNA-seq and ChIP-seq data is SRP150962.
Korn, T., Bettelli, E., Oukka, M. & Kuchroo, V. K. IL-17 and Th17 Cells. Annu. Rev. Immunol. 27, 485–517 (2009).
Wang, Z. et al. Regulatory T cells promote a protective Th17-associated immune response to intestinal bacterial infection with C. rodentium. Mucosal Immunol. 7, 1290–1301 (2014).
Basu, R. et al. IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the TH17 cell-iTreg cell balance. Nat. Immunol. 16, 286–295 (2015).
Esplugues, E. et al. Control of TH17 cells occurs in the small intestine. Nature 475, 514–518 (2011).
Zelante, T., De Luca, A., D’Angelo, C., Moretti, S. & Romani, L. IL-17/Th17 in anti-fungal immunity: what’s new? Eur. J. Immunol. 39, 645–648 (2009).
van de Veerdonk, F. L. et al. The macrophage mannose receptor induces IL-17 in response to Candida albicans. Cell Host Microbe 5, 329–340 (2009).
Lee, Y. et al. Induction and molecular signature of pathogenic TH17 cells. Nat. Immunol. 13, 991–999 (2012).
Codarri, L. et al. RORγt drives production of the cytokine GM-CSF in helper T cells, which is essential for the effector phase of autoimmune neuroinflammation. Nat. Immunol. 12, 560–567 (2011).
El-Behi, M. et al. The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM−CSF. Nat. Immunol. 12, 568–575 (2011).
Elloso, M. M., Gomez-Angelats, M. & Fourie, A. M. Targeting the Th17 pathway in psoriasis. J. Leukoc. Biol. 92, 1187–1197 (2012).
Skepner, J. et al. Pharmacologic inhibition of RORγt regulates Th17 signature gene expression and suppresses cutaneous inflammation in vivo. J. Immunol. 192, 2564–2575 (2014).
Choi, G. B. et al. The maternal interleukin-17a pathway in mice promotes autism-like phenotypes in offspring. Science 351, 933–939 (2016).
Ivanov, I. I. et al. The orphan nuclear receptor RORγt directs the differentiation program of proinflammatory IL-17+T helper cells. Cell 126, 1121–1133 (2006).
Okada, S. et al. IMMUNODEFICIENCIES. Impairment of immunity to Candida and Mycobacterium in humans with bi-allelic RORC mutations. Science 349, 606–613 (2015).
Bezbradica, J. S., Hill, T., Stanic, A. K., Van Kaer, L. & Joyce, S. Commitment toward the natural T (iNKT) cell lineage occurs at the CD4+8+stage of thymic ontogeny. Proc. Natl Acad. Sci. USA 102, 5114–5119 (2005).
Rachitskaya, A. V. et al. Cutting edge: NKT cells constitutively express IL-23 receptor and RORgammat and rapidly produce IL-17 upon receptor ligation in an IL-6-independent fashion. J. Immunol. 180, 5167–5171 (2008).
Egawa, T. et al. Genetic evidence supporting selection of the Valpha14i NKT cell lineage from double-positive thymocyte precursors. Immunity 22, 705–716 (2005).
Sun, Z. et al. Requirement for RORγ in thymocyte survival and lymphoid organ development. Science 288, 2369–2373 (2000).
Xiao, S. et al. Small-molecule RORgammat antagonists inhibit T helper 17 cell transcriptional network by divergent mechanisms. Immunity 40, 477–489 (2014).
Huang, Z., Xie, H., Wang, R. & Sun, Z. Retinoid-related orphan receptor gamma t is a potential therapeutic target for controlling inflammatory autoimmunity. Expert Opin. Ther. Targets 11, 737–743 (2007).
Sherlock, J. P. et al. IL-23 induces spondyloarthropathy by acting on ROR-γt+CD3+CD4−CD8− entheseal resident T cells. Nat. Med. 18, 1069–1076 (2012).
Langrish, C. L. et al. IL-23 drives a pathogenic T cell population that induces autoimmune inflammation. J. Exp. Med 201, 233–240 (2005).
Xie, H., Huang, Z., Wang, R. & Sun, Z. Regulation of thymocyte survival by transcriptional coactivators. Crit. Rev. Immunol. 26, 475–486 (2006).
Xie, H., Sadim, M. S. & Sun, Z. RORγt recruits steroid receptor coactivators to ensure thymocyte survival. J. Immunol. 175, 3800–3809 (2005).
Sen, S. et al. RC1 promotes Th17 differentiation by overriding Foxp3 suppression to stimulate RORγt activity in a PKC-θ-dependent manner. Proc. Natl Acad. Sci .USA 115, E458–E467 (2018).
Komander, D. & Rape, M. The ubiquitin code. Annu. Rev. Biochem. 81, 203–229 (2012).
Flotho, A. & Melchior, F. Sumoylation: a regulatory protein modification in health and disease. Annu. Rev. Biochem. 82, 357–385 (2013).
Ding, X. et al. Protein SUMOylation is required for regulatory T cell expansion and function. Cell Rep. 16, 1055–1066 (2016).
Wang, A. et al. Ubc9 Is required for positive selection and late-stage maturation of thymocytes. J. Immunol. 198, 3461–3470 (2017).
Wang, Y. & Dasso, M. SUMOylation and deSUMOylation at a glance. J. Cell Sci. 122, 4249–4252 (2009).
Wang, L. et al. SUMO2 is essential while SUMO3 is dispensable for mouse embryonic development. EMBO Rep. 15, 878–885 (2014).
Holmes, R. & Zuniga-Pflucker, J. C. The OP9-DL1 system: generation of T-lymphocytes from embryonic or hematopoietic stem cells in vitro. Cold Spring Harb. Protoc. 2009, pdb prot5156 (2009).
He, Z. et al. A two-amino-acid substitution in the transcription factor RORγt disrupts its function in TH17 differentiation but not in thymocyte development. Nat. Immunol. 18, 1128–1138 (2017).
Jager, A., Dardalhon, V., Sobel, R. A., Bettelli, E. & Kuchroo, V. K. Th1, Th17, and Th9 effector cells induce experimental autoimmune encephalomyelitis with different pathological phenotypes. J. Immunol. 183, 7169–7177 (2009).
Orta-Mascaro, M. et al. CD6 modulates thymocyte selection and peripheral T cell homeostasis. J. Exp. Med 213, 1387–1397 (2016).
Kortum, R. L., Rouquette-Jazdanian, A. K. & Samelson, L. E. Ras and extracellular signal-regulated kinase signaling in thymocytes and T cells. Trends Immunol. 34, 259–268 (2013).
Zaldumbide, A., Carlotti, F., Pognonec, P. & Boulukos, K. E. The role of the Ets2 transcription factor in the proliferation, maturation, and survival of mouse thymocytes. J. Immunol. 169, 4873–4881 (2002).
Golec, D. P., Henao Caviedes, L. M. & Baldwin, T. A. RasGRP1 and RasGRP3 are required for efficient generation of early thymic progenitors. J. Immunol. 197, 1743–1753 (2016).
Alontaga, A. Y., Bobkova, E. & Chen, Y. Biochemical analysis of protein SUMOylation. Curr. Protoc. Mol. Biol. 10, Unit10 29 (2012).
Anafi, M. et al. GCN5 and ADA adaptor proteins regulate triiodothyronine/GRIP1 and SRC-1 coactivator-dependent gene activation by the human thyroid hormone receptor. Mol. Endocrinol. 14, 718–732 (2000).
Liu, B., Tahk, S., Yee, K. M., Fan, G. & Shuai, K. The ligase PIAS1 restricts natural regulatory T cell differentiation by epigenetic repression. Science 330, 521–525 (2010).
Rutz, S. et al. Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells. Nature 518, 417–421 (2015).
Kathania, M. et al. Itch inhibits IL-17-mediated colon inflammation and tumorigenesis by ROR-gammat ubiquitination. Nat. Immunol. 17, 997–1004 (2016).
He, Z. et al. Ubiquitination of RORgammat at Lysine 446 Limits Th17 Differentiation by Controlling Coactivator Recruitment. J. Immunol. 197, 1148–1158 (2016).
Yang, J., Sundrud, M. S., Skepner, J. & Yamagata, T. Targeting Th17 cells in autoimmune diseases. Trends Pharmacol. Sci. 35, 493–500 (2014).
Tonel, G. et al. Cutting edge: a critical functional role for IL-23 in psoriasis. J. Immunol. 185, 5688–5691 (2010).
Segal, B. M. et al. Repeated subcutaneous injections of IL12/23 p40 neutralising antibody, ustekinumab, in patients with relapsing-remitting multiple sclerosis: a phase II, double-blind, placebo-controlled, randomised, dose-ranging study. Lancet Neurol. 7, 796–804 (2008).
Huh, J. R. & Littman, D. R. Small molecule inhibitors of RORgammat: targeting Th17 cells and other applications. Eur. J. Immunol. 42, 2232–2237 (2012).
Sheridan, C. Footrace to clinic heats up for T-cell nuclear receptor inhibitors. Nat. Biotechnol. 31, 370 (2013).
Guntermann, C. et al. Retinoic-acid-orphan-receptor-C inhibition suppresses Th17 cells and induces thymic aberrations. JCI Insight 2, e91127 (2017).
Liljevald, M. et al. Retinoid-related orphan receptor gamma (RORgamma) adult induced knockout mice develop lymphoblastic lymphoma. Autoimmun. Rev. 15, 1062–1070 (2016).
Zhang, F. P. et al. Sumo-1 function is dispensable in normal mouse development. Mol. Cell. Biol. 28, 5381–5390 (2008).
Robinson, J. T. et al. Integrative genomics viewer. Nat. Biotechnol. 29, 24–26 (2011).
Zhang, Y. et al. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 9, R137 (2008).
Heinz, S. et al. Simple combinations of lineage-determining transcription factors prime cis-regulatory elements required for macrophage and B cell identities. Mol. Cell 38, 576–589 (2010).
We thank Ellen Rothenberg for assisting us with the in vitro thymocyte differentiation assay, Juan Carlos Zuniga-Pflucker for providing the OP9-DL4 stroma cell line, Dan Littman for providing the RORγt antibody for the ChIP-seq assay, and Biocytogen for assisting with the design and generation of the RORγtK31R/K31R mice. We also thank the following City of Hope core facilities: the Animal Resource Center, Integrative Genomics Core, and Mass Spectrometry and Proteomics Core. This work was supported by a grant from the National Institutes of Health (R01-AI109644), institutional pilot funding, and the National Cancer Institute of the National Institutes of Health under award number P30CA33572, which specifically supports work conducted by the aforementioned core facilities at City of Hope. Z. Huang is supported by the Science and Technology Department of Guangdong Province (2017A050501010) and the Guangzhou Science Technology Innovation Commission (201807010042). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
The authors declare no competing interests.
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He, Z., Zhang, J., Huang, Z. et al. Sumoylation of RORγt regulates TH17 differentiation and thymocyte development. Nat Commun 9, 4870 (2018). https://doi.org/10.1038/s41467-018-07203-z