Fig. 6 | Nature Communications

Fig. 6

From: High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients

Fig. 6

GSK3’s involvement in steepness-dependent regulation of neuronal migration. a Fluorescence images of neurons in Sema3A gradient of varied steepness after inhibition of the GSK3 activity. Scale bar: 100 µm. b Quantitative analysis of GSK3’s role in neuronal migration and related association with the Sema3A gradient steepness, n = 3, error bars indicate standard deviation. c Box-plots for quantitative analysis of GSK3’s role in the chemorepellent guidance of neurite outgrowth in response to the varied Sema3A gradient steepness. The parts of the box indicate 25, 50, and 75 percentiles, and the whiskers indicate 5% and 95%. The square mark indicates mean of the data. More than 25 neurites (as indicated on top of each box) were pooled from three biological replicates. For b, c, the dot-lines (red) indicate logarithmic fitting of the data mean; neuronal growth pattern in each hydrogel cylinder was compared in pairwise to experiments with regular Sema3A treatment (SMA-norm), * or ** indicates a p-value < 0.05 or <0.005 by paired Kruskal–Wallis tests

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