Fig. 2 | Nature Communications

Fig. 2

From: High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients

Fig. 2

Experimental and computational characterization of gradient generation. ac Comparison of the experimental measurements (colored solid lines) and theoretical modeling (black dash lines) of the gradient profiles in the hydrogel cylinders at 7 h (a), 12 h (b), and 24 h (c) after addition of fluorescent dextran, n = 3, error bars indicate standard error of the mean. d Fluorescent images of different cross-sectional planes showing the 3D distribution of FITC-dextran molecules in the hydrogel cylinders after 12 h diffusion. Scale bar, 500 µm. e 3D reconstruction of the confocal scans showing the overall gradient generation in the HT-ChemoChip. Scale bar, 1 mm. Source data are presented as a Source Data file

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