Fig. 1 | Nature Communications

Fig. 1

From: High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients

Fig. 1

Design of the high-throughput 3D chemotactic assays. a Schematic showing the use of suspended microfluidics for generating molecular gradients with different steepness in an array of hydrogel cylinders. The enlarged inset shows the method to quantify neuronal growth, including neurite guidance and soma migration, for cells cultured in the hydrogel cylinders containing chemoattractive or chemorepulsive cues. b The step-by-step protocol for device assembly and its application to high-throughput 3D chemotactic assays

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