Fig. 7 | Nature Communications

Fig. 7

From: Structure of the 4-1BB/4-1BBL complex and distinct binding and functional properties of utomilumab and urelumab

Fig. 7

Urelumab activates 4-1BB signaling more strongly than utomilumab. Human 4-1BB NF-ĸB luciferase reporter HEK293 cells were incubated with fivefold increasing concentrations (max 250 nM) of a utomilumab or b urelumab antibody at 37 °C for 6 h before measuring luciferase activity and comparing to unstimulated cells. Mean fold induction was plotted against concentrations of test antibodies. Error bars represent the propagated standard deviation (SD) of three measurements. Solid lines are fits with dose-response curves with maximal fold induction of 1.38 ± 0.051 and 1.84 ± 0.053 for utomilumab and 1.99 ± 0.046 and 2.24 ± 0.033. c, d Purified human CD8 T cells were stimulated with plate bound anti-CD3 (50 ng/ml) plus each plate bound antibody with fourfold increasing concentrations (max 333.33 nM) at 37° C for 72 h and human cytokine production was measured by ELISA. Error bars represent the standard deviation of two measurements. All cytokines are measured from the same donor

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