Fig. 1 | Nature Communications

Fig. 1

From: Perineuronal nets decrease membrane capacitance of peritumoral fast spiking interneurons in a model of epilepsy

Fig. 1

Glioma-mediated neuronal cell loss with a preferential vulnerability of PV interneurons. a Representative images of NeuN (red), PV (green), and WFA (yellow) labeling, top to bottom, in glioma-injected (left) and sham-injected (right) cortical slices. Glioma mass (bottom left) can be discerned by highly dense DAPI-positive cell nuclei delimited by dashed line. Scale = 100 µm. b Mean density of NeuN+ neurons at 0.0–0.2 mm (9.25 ± 1.63), 0.2–0.4 mm (15.25 ± 0.94), and 0.4–0.6 mm (19.06 ± 1.28) distance from the glioma mass compared to equivalent position in contralateral (25.41 ± 0.68) and sham animals (26.12 ± 0.94). n = 6 mice. c Summary of PV+ neuron cell density at 0.0–0.2 mm (0.58 ± 0.1), 0.2–0.4 mm (0.77 ± 0.14), and 0.4–0.6 mm (1.2 ± 0.2) distance from the glioma mass compared to contralateral (2.52 ± 0.15) and sham (2.6 ± 0.25). n = 6 mice. d WFA+ PNNs in the PTC at 0.0–0.2 mm (0.87 ± 0.16), 0.2–0.4 mm (0.87 ± 0.1), and 0.4–0.6 mm (1.48 ± 0.14), from glioma mass compared to contralateral (2.75 ± 0.13) and sham (2.78 ± 0.17). n = 6 mice. e Normalized density of PV+ and NeuN+ neurons at 0.2–0.4 mm (PV+, 29.75 ± 5.45%; NeuN+, 58.39 ± 3.62%), and 0.4–0.6 mm (PV+, 46.18 ± 7.7%; NeuN+, 72.99 ± 4.88%) distance from the tumor border. No significant difference was observed in the density of PV+ and NeuN+ neurons in contralateral (PV+, 96.91 ± 5.90%; NeuN+, 97.29 ± 2.6%) and sham-injected animals (PV+, 100 ± 9.58%; NeuN+, 100 ± 3.62%). Normalized density of WFA+ PNNs in the 0.2–0.4 mm (PNN+, 31.37 ± 3.66%; NeuN+, 58.38 ± 3.61%) was significantly different compared to NeuN. n = 6 mice. Cell density was normalized to sham. Bar data represent mean ± SEM unless otherwise stated. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, one-way ANOVA, Tukey’s post-hoc test in (bd); and two-way ANOVA, Tukey’s post-hoc test in (e)

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