Fig. 6 | Nature Communications

Fig. 6

From: Exosomes derived from HIV-1-infected cells promote growth and progression of cancer via HIV TAR RNA

Fig. 6

TLR3 is involved in tumor-promoting function of HIV-positive exosomes. a HSC3 cells pretreated with the TLR3/dsRNA inhibitor (6 µM, +Inh) for 30 min, or remained untreated, and then transfected with TAR RNA (1 µg ml−1). Total RNA was used for qRT-PCR of IFIT1 and IFNB1 mRNA. Error bars, ± s.d., n= 3, *p< 0.02, F-test. Data represent one experiment from three independent repeats. b Immunoblot of HSC3 cancer cells treated with exosomes (2 × 109 ml−1) from J1.1 or Jurkat cells in the presence or absence of the TLR3/dsRNA inhibitor (6 µM, TLR3 inh). Uncropped blot images in Supplementary Figure 4g. c Immunoblot of wild-type or TLR3-KO HSC3 cells treated with J1.1 or Jurkat cell exosomes at 2 × 109 ml–1. Right, densitometry of immunoblots over that of control. Error bars, ± s.d. Uncropped blot images in Supplementary Figure 4h. d Wild-type (Ctrl) and TLR3-KO (TLR3KO) HSC3 cancer cells were treated with exosomes (4 × 109 ml−1) from J1.1 or Jurkat cells for proliferation assays. Data (mean ± s.d., n= 3) represent one experiment from three independent repeats; *p< 0.05, one-way ANOVA on days 3 to 5. e Tumor growth of wild type and TLR3-KO HSC3 (TLR3KO) cancer cells inoculated into nude mice in the presence of J1.1 exosomes (J1.1 exo, 6.3 × 109 ml−1). HSC3 cells inoculated with Jurkat exosomes (Jurkat exo, 6.3 × 109 ml−1) were used as control. Error bars represent ± s.d., n= 12, *p < 0.01 and **p< 0.007, Student’s t-test

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