Fig. 5 | Nature Communications

Fig. 5

From: Exosomes derived from HIV-1-infected cells promote growth and progression of cancer via HIV TAR RNA

Fig. 5

J1.1 cell exosomes stimulate ERK1/2 phosphorylation via EGFR. a HSC3 cells were treated with U0126 (5 µM, + U0126) or cetuximab (20 µg ml−1, +Cet) for 30 min or remained untreated, followed by stimulation with exosomes (4 × 109 ml−1) from J1.1 or Jurkat cells for cell proliferation. Ctrl, serum-free medium; J1.1 and Jurkat, exosomes from J1.1 and Jurkat cells, respectively. Error bars, ± s.d., n= 4, *p< 0.05, one-way ANOVA on days 3 to 5. b HSC3 cell invasion in response to exosomes (4.2 × 109 ml−1) from Jurkat or J1.1 cells in the absence or presence of U0126 (5 µM, +U0126) or cetuximab (20 µg ml−1, +Cet). Data (mean ± s.d., n= 3) represent one experiment out of three independent repeats; *p< 0.05 and **p< 0.02, F-test. c HSC3 HNSCC and H1299 lung cancer cells treated with exosomes (2 × 109 ml−1) from Jurkat or J1.1 cells in the absence or presence of cetuximab (20 µg ml−1, Cet) for 10 min, followed by immunoblotting on total cell lysates. EGF (10 ng ml−1), positive control; kDa, protein marker size. Uncropped blot images in Supplementary Figure 4e. d Proliferation of B-cell lymphoma cell lines SU-DHL-16, Toledo, and Pfeiffer in response to exosomes (4 × 109 ml−1) isolated from J1.1 or Jurkat cells and EGF (10 ng ml−1). Data (mean ± s.d., n = 4) represent one experiment from two independent repeats. NS, p > 0.05, one-way ANOVA on days 3 to 5. e Immunoblot of total EGFR (EGFR), TLR3, and CD19 using total cell lysates from SU-DHL-16, Pfeiffer, Toledo, and H1299 cells. Uncropped blot images in Supplementary Figure 4f

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