Fig. 2: | Nature Communications

Fig. 2

From: Exosomes derived from HIV-1-infected cells promote growth and progression of cancer via HIV TAR RNA

Fig. 2

HIV-infected T-cell exosomes promote cancer cell proliferation and migration. a The effect of exosomes on cell proliferation of HSC3, SCC9, and UM-SCC-104 HNSCC cells treated with 4 × 109 exosomes ml−1 from J1.1 (J1.1 Exo) or Jurkat (Jurkat Exo) cells. Ctrl, serum-free medium. Error bars, ± s.d., n = 3, *p< 0.01, one-way ANOVA on days 3 to 5. b HSC3 cancer cell migration in response to J1.1 (J1.1 Exo) or Jurkat cell exosomes (Jurkat Exo) (4.2 × 109 ml−1). Error bars, ± s.d., n= 4, *p< 0.02, F-test. EGF (10 ng ml−1), positive control. Relative migration was determined by migration of treatment over that of control. c Transwell invasion assays of HSC3 cells in response to J1.1 (J1.1 Exo) or Jurkat cell exosomes (Jurkat Exo) (4.2 × 109 ml−1) loaded in the lower chamber of the transwell plate. EGF (10 ng ml−1), positive control. Error bars, ± s.d., n = 4, *p< 0.02, F-test. d Tumor weight of UM-SCC-104 and HSC3 cell xenografts in nude mice. Cancer cells (5 × 106) were mixed with 1.6 × 1010 ml−1 of J1.1 or Jurkat cell exosomes and then inoculated into nude mice for the xenograft experiment. Data represent means ± s.d., n= 12, *p < 0.05 and **p< 0.02, Student’s t-test. Data represent one experiment from at least three biological repeats in a, b and c