Fig. 9 | Nature Communications

Fig. 9

From: Self-implantable double-layered micro-drug-reservoirs for efficient and controlled ocular drug delivery

Fig. 9

Inflammation assessment of combinational therapy using DL-MN patch. Mouse eyes were treated differently 2 days after being inflicted with alkali-burn, and examined at day 7. a Immunohistochemical staining of the cornea with a specific macrophage marker—F4/80 surface antigen (green colour). Scale bar = 50 µm. b Quantifications of macrophage accumulation (% of positive staining of F4/80; ED: eye drop) (n = 4). cf Cytokine concentrations in collected tear film of differently treated eyes. Tear films were collected with MeHA-based MN-free eye patches (patches were put on the eyes for 1 min to absorb tear film). c Scale bar = 500 µm. d Scanning electron microscopy image of the patch soaked with PBS (scale bar = 10 µm), and confocal image of the patch with cy5-conjugated albumin absorbed from agarose hydrogel (scale bar = 200 µm). Cytokine concentrations (IL6 and VEGF) were measured with specific ELISA kits (e, f) (n = 5). Data represents mean ± SEM. n represents the number of samples for each group. Statistical comparison between groups was performed using one way ANOVA. *p < 0.05, **p < 0.01 vs. control (no treatment on alkali-burn eyes); #p < 0.05, ##p < 0.01 between indicated pairs

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