Fig. 3 | Nature Communications

Fig. 3

From: Self-implantable double-layered micro-drug-reservoirs for efficient and controlled ocular drug delivery

Fig. 3

Characterization of double-layered microneedles (DL-MNs). a, b Schematic and SEM images of a polymeric patch with an array of DL-MNs (the outer layer made of crosslinked MeHA and inner core made of HA). Scale bar = 100 µm. c–e Representative confocal images of c DL-MNs loaded with immunoglobulin G conjugated with Alexa Fluor: IgG(680) (red colour) in outer layer and IgG(488) (green colour) in inner core, d DL-MNs with IgG(680) in outer layer only, e DL-MN with IgG(488) in inner core only. Scale bars = 100 µm. f In vitro fast and slow release profiles of DL-MNs in simulated tears or gelatin hydrogel (37 °C). IgG(680) was loaded in outer layer and IgG(488) was loaded in inner core (n = 3). g In vitro release profiles of IgG(680) from HA-MN, MeHA-MN or DL-MN in phosphate buffer saline (PBS) (37 °C) (n = 3). IgG(680) was loaded in both layers of DL-MN. h Mechanical compression test of HA-MN, MeHA-MN and DL-MN (n = 4). The data represents as mean value, and error bars indicate SEM (mean ± SEM). n represents the number of samples for each group

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