Fig. 4 | Nature Communications

Fig. 4

From: Mycoplasma genitalium adhesin P110 binds sialic-acid human receptors

Fig. 4

Affinity of oligosaccharides by SPR. Characterization of M. genitalium P110 variants. a SPR sensorgrams showing the binding of the erP110 β-propeller N-domain to immobilized 3SL and 6SL (upper and lower left panels, respectively). Data fitting of duplicates assuming a Langmuir 1:1 affinity model depicting the KD calculated in steady state for 3SL and 6SL (upper and lower right panels, respectively). b SDS–PAGE of whole cell lysates from WT and different P110 mutant strains. c HA assays determined by FACS analysis. Graphic depicts inverse Langmuir plots obtained with a fixed amount of mycoplasma cells and increasing amounts of hRBCs. Plots were generated using data from, at least two biological repeats for each strain. d HA assays of the WT strain using neuraminidase-treated hRBCs

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