Fig. 1 | Nature Communications

Fig. 1

From: Dynamics of individual molecular shuttles under mechanical force

Fig. 1

Molecular design and experimental setup. a The chemical structure of the molecular shuttle consists of a benzylic tetraamide macrocycle (red) locked onto an axle (blue) terminated by diphenylethyl groups at each end (stoppers). The axle contains fumaramide (fum, green) and succinic amide-ester (succ, orange) stations separated by a long oligoethyleneglycol spacer. At low forces the macrocycle resides predominantly over the fum station. A DNA oligonucleotide was covalently attached to the macrocycle to allow for manipulation in the optical tweezers. b The molecular shuttle is attached to two polystyrene beads (diameter ~3 μm) through two dsDNA molecules (not to scale): The 2686 bp dsDNA molecule connects the macrocycle to the bead in the optical trap (k = 0.13 pN nm−1, laser power = 60 mW) via digoxigenin-antidigoxigenin (Dig-αDig) connections. The 830 bp dsDNA molecule connects the shuttle to a bead held by suction on a micropipette, via biotin-streptavidin connections at one end and Dig-αDig at the other end. Load is applied to the system by moving the optical trap away from the micropipette. At constant load, the shuttling dynamics of the macrocycle are inferred by measuring the motions of the bead in the optical trap

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