It is well known that high-risk human papilloma virus (HR-HPV) infection is strongly associated with cervical cancer and E7 was identified as one of the key initiators in HPV-mediated carcinogenesis. Here we show that lactate dehydrogenase A (LDHA) preferably locates in the nucleus in HPV16-positive cervical tumors due to E7-induced intracellular reactive oxygen species (ROS) accumulation. Surprisingly, nuclear LDHA gains a non-canonical enzyme activity to produce α-hydroxybutyrate and triggers DOT1L (disruptor of telomeric silencing 1-like)-mediated histone H3K79 hypermethylation, resulting in the activation of antioxidant responses and Wnt signaling pathway. Furthermore, HPV16 E7 knocking-out reduces LDHA nuclear translocation and H3K79 tri-methylation in K14-HPV16 transgenic mouse model. HPV16 E7 level is significantly positively correlated with nuclear LDHA and H3K79 tri-methylation in cervical cancer. Collectively, our findings uncover a non-canonical enzyme activity of nuclear LDHA to epigenetically control cellular redox balance and cell proliferation facilitating HPV-induced cervical cancer development.
Cervical cancer is the third most common cancer in women worldwide with about 528,000 new cases and 266,000 deaths annually1. Among those, about 95% cases are caused by persistent infections with HR-HPVs2. During high-risk HPV infection, two viral early genes, E6 and E7, were identified to play key roles in carcinogenesis by regulating signaling pathways related to cellular transformation3,4. Moreover, recent studies reported that HPV16 E7 was the more potent driver for cervical cancer5,6,7,8,9, and elevation of HPV16 E7 was required to sustain a malignant phenotype in primary cervical cancer10. HPV16 E7 has been shown as an essential factor for viral replication in human keratinocytes11. And yet, the new mechanism of E7 protein on HPV-induced cervical carcinogenesis remains to be discovered.
Cellular ROS are elevated during the process of many virus infections to host cells, including Epstein–Barr virus (EBV)12, hepatitis C virus (HCV)13, hepatitis B virus (HBV)14, and HPVs15,16. The virus-induced chronic oxidative stress increases the integration frequency of HPV16 DNA in human keratinocytes17. However, excessive ROS levels are fatal to host cells. It is necessary to increase the antioxidant capacity to counteract with the excessive ROS. In response to oxidative stress, nuclear factor erythroid-derived 2-like 2 (NRF2), a key regulator of cellular antioxidant responses, plays an important role in antioxidant defense through transcriptional activation of antioxidant-response-element (ARE)-bearing genes18,19. However, it remains unclear how HPVs control the highly elevated intracellular ROS levels.
Altered cell metabolism is one of the hallmarks of cancer20. Metabolism is subtly orchestrated in cancer cells, one frequent phenomenon being a shift from respiration to aerobic glycolysis (known as “Warburg effect”)21. Early studies suggested that HPV16 E7 oncoprotein affected cellular metabolism on transcription levels22. Moreover, inhibition of hexokinase 2 (HK2), the first rate-limiting enzyme of glycolysis, contributed to suppressing HPV16 E7-induced glycolytic phenotype of tumor metabolism and inhibited tumor cell growth23. Enhanced expression of LDHA, which is needed for maintaining glycolysis and other metabolic activities, has been associated with the evolution of aggressive and metastatic cancers in a variety of tumor types, including cervical cancer24. Silencing of LDHA significantly attenuated colony-formation ability and invasive capacity of cervical cancer cells25. Here, we report that LDHA translocates into the nucleus in response to HPV16/18 E7, which depends on elevated ROS levels, and gains a noncanonical enzyme activity to produce an antioxidant metabolite, α-hydroxybutyrate (α-HB). The accumulation of α-HB further activates antioxidant response and Wnt signaling through epigenetic modifications. We present a molecular mechanism that explains how HR-HPVs promote cervical cancer development through nuclear translocation of LDHA and the product of noncanonical enzyme activity, α-HB, providing a crosstalk between cell metabolism and epigenetic signaling networks.
HPV-induced LDHA nuclear translocation is dependent on ROS
HPV infection is the most common factor of cervical cancer. Among all HR-HPVs, HPV16 is the most carcinogenic HPV type and accounts for ~55%–60% of all cervical cancers, and HPV18 is the second type which accounts for 10%–15%26. Persistent oxidative stress is always accompanied by the process of HPV infection to host cells15,16. The altered cellular redox levels may cause reversible modifications on specific cysteine residues and affect the protein functions27,28,29. To decipher the changes in the cysteine proteome on HR-HPV infection, we developed a sensitive and specific redox proteomics method using iodoacetyl tandem mass tag (iodoTMT) reagents composed of a sulfhydryl-reactive iodoacetyl group selective labeling sulfhydryl (-SH) groups and sets of isobaric isomers which can be differentiated by mass spectrometry (MS), enabling quantitation of the relative abundance of cysteine modifications (Supplementary Fig. 1a). To identify HPV-related redox-sensitive effectors, the cysteine proteomes were obtained from C33A (HPV negative), SiHa (containing HPV16 genome), and HeLa (containing HPV18 genome) cells (Supplementary Fig. 1b). The key glycolysis enzyme LDHA was identified to be a potential key regulator in HPV-induced cervical cancer development (Supplementary Data 1).
To investigate the potential role of LDHA in HPV-induced cervical cancer, we collected HPV-negative and HPV-positive cervical cancer specimens from 66 patients (Supplementary Data 2). Immunohistochemistry (IHC) showed that LDHA nuclear staining was significantly increased in HPV16-positive tumors compared with HPV16-negative tumors (Fig. 1a, b). To test whether the pivotal oncoprotein HPV16/18 E7-mediated LDHA nuclear translocation, we packaged retrovirus containing HPV16/18 E7 gene and infected primary human cervix keratinocytes (PHKs), immortalized human keratinocyte cell line HaCaT, and transfected HPV16 E7 gene into HPV-negative human cervical cancer cell line HT-3 (Supplementary Fig. 2a). As expected, HPV16/18 E7 expression dramatically increased the percentage of LDHA nuclear-translocated cells from ~5% to ~50% (Fig. 1c, d, and Supplementary Fig. 2b, c). In line with the potential effect of HPV infection on ROS production, we found that HPV16/18 E7 induction resulted in cellular ROS accumulation (Fig. 1e and Supplementary Fig. 2d). Notably, supplement with a ROS scavenger N-acetyl-L-cysteine (NAC) remarkably reduced LDHA nuclear translocation in HPV16/18 E7-transduced cells (Fig. 1c, d, and Supplementary Fig. 2b, c). This observation triggered us to speculate that ROS possibly promote LDHA nuclear translocation. To this end, we treated HaCaT, HT-3, U2OS, and HeLa cells with hydrogen peroxide (H2O2) and found that LDHA rapidly translocated from the cytoplasm to nuclear in a dose-dependent manner, and the H2O2-induced subcellular redistribution of LDHA was reversed by NAC supplement (Fig. 1f, g, and Supplementary Fig. 3a–d). Meanwhile, the cellular ROS levels were measured upon H2O2 and NAC treatment in HT-3 and U2OS cells under the same condition (Supplementary Fig. 3e). To further validate this, we performed nuclear isolation assay and found the similar pattern for LDHA localization (Fig. 1h). These data indicated that LDHA nuclear translocation induced by HPV infection is dependent on ROS.
Mitochondrial respiratory chain and nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs) contribute the major source of endogenous ROS in mammals30,31. To figure out the potential source of ROS induced by HPV16/18 E7, mitochondrial and NOXs originated ROS were measured, respectively. As shown in Supplementary Fig. 4a, HPV16/18 E7 expression increased mitochondrial ROS generation, as determined using the fluorescent dye MitoSOX Red. On the other hand, NOXs inhibitor diphenyleneiodonium chloride (DPIC) decreased the intracellular ROS level (Supplementary Fig. 4b), suggesting that NOXs contributes to HPV16/18 E7-induced intracellular ROS elevation as well as mitochondrial respiratory chain.
Nuclear LDHA gains a noncanonical activity to produce α-HB
Lactate dehydrogenase acts an indispensable role in cancer development and possesses a well-defined canonical enzyme activity, catalyzing the conversion between pyruvate (Pyr) and lactate (Lac)32. It is well known that LDHA localizes at cytoplasm exerting its lactate-producing activity. Interestingly, LDHA has also been shown to exhibit noncanonical enzyme activities33,34,35. We hypothesized that, after nuclear translocation, LDHA probably gained a noncanonical enzyme activity. To this end, we discovered that LDHA acquired a α-HB-producing activity in the nucleus (Fig. 2a and Supplementary Fig. 5). We measured both the canonical and noncanonical enzyme activities upon H2O2 exposure or HPV16 E7 induction, with or without NAC supplement. Interestingly, the noncanonical enzyme activity but not the canonical activity of LDHA was elevated by ~1.5-fold after HPV16 E7 induction or H2O2 treatment (Fig. 2b, c). Moreover, NAC supplement decreased noncanonical enzyme activity of LDHA, suggesting a ROS-dependent switch in LDHA activity. In addition, we quantified four direct substrates of LDHA, including pyruvate, lactate, α-ketobutyrate (α-KB), and α-HB, using liquid chromatography coupled to triple quadrupole tandem mass spectrometry (LC-MS/MS). As expected, α-HB, the product of noncanonical LDHA activity, accumulated by ~1.6-fold after HPV16/18 E7 induction or H2O2 treatment, while no significant changes were observed on pyruvate or lactate levels (Fig. 2d, e, and Supplementary Fig. 6a–e). Notably, NAC supplement totally blocked the elevation of α-HB (Fig. 2d, e, and Supplementary Fig. 6d, e). To assess the function of nuclear LDHA, we isolated nuclei using a well-established, nuclei-specific high-sucrose gradient centrifugation protocol for metabolite measurement (Supplementary Fig. 6b)36. Consistent with whole-cell data above, nuclear α-HB was also accumulated upon HPV16/18 E7 induction and H2O2 treatment while NAC supplement eliminated this induction in HaCaT and HT-3 cells (Supplementary Fig. 6c–e). Furthermore, the accumulation of α-HB was diminished in LDHA-knockout (KO) cells (Supplementary Fig. 6f, g), indicating that the noncanonical enzyme activity of LDHA mediates its response to ROS via producing α-HB.
To further test whether the upregulation of noncanonical enzyme activity was a consequence of LDHA nuclear translocation, we generated stable cells with endogenous LDHA knockdown and putting back with shLDHA resistant flag-tagged vector, wild-type LDHA (WT) and its mutants containing nuclear localization signal (LDHANLS) and nuclear export signal (LDHANES) peptides, respectively37 (Supplementary Fig. 7). Consistently, both elevated noncanonical LDHA enzyme activity and α-HB accumulation were observed in LDHANLS stable cells (Fig. 2f, g). Taken together, these data demonstrate that nuclear LDHA gains a noncanonical enzyme activity, leading to accumulation of α-HB.
ROS disrupt LDHA tetramer to promote noncanonical activity
To examine whether the LDHA nuclear translocation was associated with LDHA oligomerization, protein crosslinking assay and gel filtration were performed. LDHA tetramers were dramatically decreased by H2O2 treatment, accompanied by increased dimer and monomer (Fig. 3a). Along with the expression of HPV16 E7 increased LDHA dimer to ~1.9-fold in HaCaT cells and ~1.5-fold in HT-3 cells, respectively (Supplementary Fig. 8a). Next, we fractionated cell extracts by gel filtration and determined the distribution of endogenous LDHA by western blotting. LDHA distributed broadly in multiple fractions but fewer portions were found in dimeric fractions (as determined using protein standards shown in Supplementary Fig. 8b) under normal condition (Fig. 3b and Supplementary Fig. 8c). Upon H2O2 treatment, LDHA significantly shifted into dimer fractions (Fig. 3b, lower panel). As the oligomerization state of metabolic enzymes closely links to their catalytic activity38,39,40, we measured both canonical and noncanonical enzyme activities of tetrameric and dimeric fractions of LDHA. Strikingly, the noncanonical enzyme activity of dimer fractions was significantly increased by more than 1.7-folds while canonical enzyme activity decreased > 3.8-folds compared with that of tetrameric fraction (Fig. 3c). Furthermore, LDHANLS group presented much more dimer fractions than LDHANES group (Fig. 3d, e, and Supplementary Fig. 8d, e). Taken together, these results demonstrate that ROS-induced nuclear translocation of LDHA, accompanied with its tetramer-to-dimer transition, confers LDHA with a noncanonical enzyme activity.
α-HB accumulation induces H3K79 hypermethylation
Aberrant metabolic alterations have been widely associated with tumorigenesis, while the landscape of histone methylation is remodeled with altered metabolism41,42. To explore the biological function of intracellular α-HB accumulation, we examined the dimethylation levels of six common histone methylation markers, H3K4, H3K9, H3K27, H3K36, H3K79, and H4K20, in HaCaT cells upon α-HB treatment. Histone H3K79 methylation levels were shown to be upregulated by α-HB in a dose and time-dependent manner (Supplementary Fig. 9a). To confirm the specific effect of α-HB on H3K79 methylation, mono-, di-, trimethylation levels were determined after cells treated with four LDHA-related metabolites in our study (α-HB, α-KB, Pyr, and Lac). Notably, α-HB caused a significant upregulation of histone H3K79 methylation (Fig. 4a). We further determined whether the impact of H2O2 treatment or HPV16/18 E7 transduction on H3K79 hypermethylation. As shown in Fig. 4b, c, and Supplementary Fig. 9b, histone H3K79 trimethylation level was significantly increased upon HPV16/18 E7 induction or H2O2 treatment, which was partially reversed by NAC. We next defined whether H3K79 hypermethylation was dependent on LDHA nuclear translocation by using LDHA mutants. Cells expressing LDHANLS, but not LDHANES, showed elevated H3K79 trimethylation level (Fig. 4d). More importantly, supplementation of α-HB to LDHANES cells remarkably recovered H3K79 trimethylation level (Fig. 4d), suggesting that the noncanonical enzyme activity of nuclear LDHA is required for H3K79 hypermethylation.
To test whether H3K79-specific methyl-transferase DOT1L was involved in α-HB-induced H3K79 hypermethylation, we adopted a DOT1L-specific inhibitor, EPZ00477743, to delineate how LDHA-modulated H3K79 hypermethylation. EPZ004777 treatment blocked LDHANLS-induced upregulation of H3K79 trimethylation (Fig. 4d and Supplementary Fig. 9c), indicating that DOT1L was necessary for nuclear LDHA to increase H3K79 methylation. To explore the mechanism underlying nuclear LDHA-induced H3K79 hypermethylation, coimmunoprecipitation was performed to determine the interaction between DOT1L and LDHA upon HPV16/18 E7 transduction or H2O2 treatment. Notably, both HPV16/18 E7 transduction and H2O2 treatment strengthened the binding of DOT1L with LDHA, which was partially blocked by NAC supplement (Fig. 4e, f, and Supplementary Fig. 9d). Given that treatment of α-HB to LDHANES cells restored H3K79 trimethylation, we hypothesized that α-HB produced by nuclear LDHA might take part in the activation of DOT1L though its binding to LDHA. To test this, we found that treatment of cells with α-HB alone increased the binding between endogenous DOT1L and LDHA (Fig. 4g). Overall, these results suggested that α-HB produced by nuclear LDHA induces H3K79 hypermethylation through promoting the interaction between DOT1L and LDHA, which possibly activates the methyl-transferase activity of DOT1L.
NRF2 is required for LDHA-induced antioxidant responses
High ROS levels are generally detrimental to cells, and the increased antioxidant capacity becomes vital to maintaining tumor development and cell survival. In addition, aberrant activation of Wnt/β-catenin signaling pathway, which is frequently observed in human cervical cancer, promotes cell proliferation and tumor progression44,45. Interestingly, the activation of DOT1L has been reported to modulate Wnt target genes expression46,47. To test whether the H3K79 hypermethylation induced by HPV16 E7 expression or H2O2 treatment further regulated cellular antioxidant responses or cell proliferation, seven antioxidant genes and three Wnt target genes were subjected to qPCR analysis. As expected, HPV16 E7 expression and H2O2 treatment increased the expression of the majority of these genes, which was blocked by NAC (Fig. 5a and Supplementary Fig. 10a, b). Furthermore, ChIP-qPCR assays were carried out to define the histone methylation status of these HPV16/18 E7-induced target genes. We found that HPV16/18 E7 markedly enhanced H3K79 dimethylation level at the SOD1, CAT, CTNNB1, and MYC gene body, while NAC supplement reversed this effect (Fig. 5b and Supplementary Fig. 10c, d). These data suggest that HPV16/18 E7-induced H3K79 hypermethylation upregulates antioxidant genes and Wnt target genes expression in a ROS-dependent manner.
Several crucial transcription factors are enrolled in cellular redox sensing, such as NRF2, hypoxia-inducible factor 1α (HIF-1α), and nuclear factor kappa B (NF-κB)19,48. To further identify the key effector that directly mediated the antioxidant responses corresponding to high H3K79 methylation level, the expression of NRF2, HIF-1α, and NF-κB target genes were analyzed by qPCR. In response to HPV16 E7 expression and H2O2 treatment, the mRNA expression of NRF2 (NQO1, GCLC), HIF-1α (SLC2A1, VEGFA), and NF-κB (IL-10, TNFB1) target genes expression were remarkably increased (Fig. 5c and Supplementary Fig. 11a). However, EPZ004777 treatment blocked the upregulation of NRF2 target genes (NQO1 and GCLC), but not HIF-1α or NF-κB target genes (Fig. 5c and Supplementary Fig. 11a). This data indicates that NRF2, not HIF-1α or NF-κB, may be the key transcription factor during HPV16 E7 or H2O2-induced H3K79 hypermethylation. Of note, we confirmed the increase of NRF2 protein level as a molecular marker of the NRF2 pathway activation upon HPV16/18 E7 expression (Supplementary Fig. 11b). We further validate the role of NRF2 in H2O2 or HPV16/18 E7-induced antioxidant genes expression. Increased mRNA expression of NQO1 and GCLC was dramatically impaired by deletion of NRF2 or ML385, a NRF2-specific inhibitor49 (Fig. 5d, e, and Supplementary Fig. 11c, d). To further test if LDHA is essential for the antioxidant gene activation, LDHA KO and LDHA, NRF2 double-knockout (DKO) HeLa cells were generated for qPCR analysis. As shown in Fig. 5f and Supplementary Fig. 11e, LDHA KO significantly reduced NQO1 and GCLC expression, but not the expression of HIF-1α or NF-κB target genes, upon H2O2 treatment. In addition, activation of antioxidant genes was markedly decreased in DKO cells (Supplementary Fig. 11f, g). Genetic ablation of LDHA remarkably blocked the increase of H3K79 dimethylation level of SOD1, CAT, CTNNB1, and MYC, while deletion of NRF2 blocked the increase of H3K79 dimethylation level of SOD1 and CAT, but not CTNNB1 and MYC gene body (Fig. 5g). Together, these data unveil a LDHA-DOT1L-NRF2 axis in HPV16/18 E7 and H2O2-induced antioxidant responses.
Nuclear LDHA produced α-HB counteracts with oxidative stress
Given the observed activation of antioxidant and Wnt signaling pathways, we presumed that nuclear LDHA enables cell survival and proliferation under oxidative stress. First, we examined the effect of nuclear LDHA on cell proliferation and colony formation in LDHAWT, LDHANLS, and LDHANES cells, respectively. There were no significant changes in cell growth between LDHAWT, LDHANLS, and LDHANES stable cells under normal condition (Fig. 6a). However, LDHAWT and LDHANLS stable cells demonstrated significant growth advantage and colony-formation ability under continuous oxidative stress compared with vector control and LDHANES cells (Fig. 6a–c and Supplementary Fig. 12a). Next, we examined the expression of antioxidant genes and Wnt target genes in LDHAWT, LDHANLS, and LDHANES cells in response to H2O2. On one hand, LDHANLS cells presented higher expression levels of these genes under normal condition. On the other hand, LDHAWT but not LDHANES cells showed more pronounced target genes expression in response to H2O2 (Fig. 6d and Supplementary Fig. 12b). Consistently, we observed that LDHANLS cells presented a lower level of ROS (Fig. 6e). Collectively, LDHA nuclear translocation is required for the activation of antioxidant genes and Wnt target genes.
As mentioned above, supplement of α-HB to LDHANES cells remarkably recovered H3K79 trimethylation level. We thus speculated that α-HB potentially rescued the antioxidant ability of LDHANES cells. Interestingly, the addition of α-HB remarkably relieved the ROS accumulation (Fig. 6f). In accordance, α-HB supplement significantly upregulated the expression of antioxidant genes and Wnt target genes (Fig. 6g). α-HB supplement effectively rescued cell growth and colony formation in LDHANES stable cells under continuous oxidative stress in a dose-dependent manner (Fig. 6h, i, and Supplementary Fig. 12c), suggesting that α-HB contributes to, at least in part, LDHA-mediated overcoming of oxidative stress. Collectively, these observations strongly support that LDHA nuclear translocation, in response to HPV induction or ROS stimulation, maintains redox homeostasis and cell survival under stressed conditions. LDHA gain-of-function that produces the antioxidant metabolite, α-HB, is critical for mediating antioxidant response.
Nuclear LDHA implicates in cervical cancer development
To examine the effect of nuclear LDHA in tumor growth, LDHA KO with vector, LDHAWT, LDHANLS, and LDHANES rescue cells were injected subcutaneously into nude mice. Consistent with previous reports32, LDHAWT group showed increased tumor growth compared to vector group, while LDHANLS but not LDHANES group displayed significantly increased tumor growth compared with other groups (Fig. 7a–c). This result suggests that nuclear LDHA promotes tumor growth. In agreement, elevated NRF2, SOD1, MYC, and H3K79 trimethylation levels were observed in LDHANLS xenografts (Fig. 7d).
To examine the effect of HPV16-induced LDHA nuclear translocation in vivo, we adopted a K14-HPV16 transgenic mouse model with which direct cervical application of HPV16 E7-targeted transcription activator-like effector nucleases (TALENs) effectively mutated the E7 oncogene, reduced viral DNA load, and restored retinoblastoma-associated protein (RB1) function50. Along with the TALEN applied, the cervical epithelium of the K14-HPV16 mouse showed a gradual loss of E7 and a reduction of epithelial proliferation. Meanwhile, the gradual loss of LDHA nuclear translocation correlated well with the decrease of H3K79 trimethylation level in serial sections (Fig. 7e). Strikingly, on day 24 when E7 expression was almost undetectable, nuclear LDHA was eliminated and H3K79 trimethylation levels were dramatically attenuated (Fig. 7e). These in vivo data strongly support that HPV16 E7 induces LDHA nuclear translocation and H3K79 hypermethylation.
To further define the clinical relevance of our findings that HPV16 E7-induced LDHA nuclear translocation activated antioxidant response and Wnt signaling pathway, IHC analyses were performed to examine HPV16 E7 expression, LDHA nuclear localization, and H3K79 trimethylation levels in serial sections of 52 cases of human primary cervical cancer specimens. As shown in Fig. 7f and Supplementary Fig. 13, levels of HPV16 E7, nuclear LDHA expression, and H3K79 trimethylation were significantly positively correlated with each other, strongly supporting the crucial role of nuclear LDHA in HPV-positive cervical cancer.
LDHA is implicated in tumorigenesis and tumor development32,51,52,53. Loss of LDHA resulted in an elevated ROS production (Fig. 6e) and a concomitant decrease in cell proliferation and invasion54,55. Here, we have demonstrated a striking and previously unknown mechanism that nuclear LDHA senses ROS and gains a new catalytic function to promote HPV-induced cervical carcinogenesis: in response to HPV infection or oxidative stress, LDHA senses excessive ROS with a tetramer-to-dimer transition and nuclear translocation. Nuclear LDHA gains a noncanonical enzyme activity to produce an antioxidant metabolite, α-HB, which can protect cervical cancer cells from excessive oxidative stress and promote cell growth through epigenetic regulations. Interestingly, LDHA nuclear translocation appears to be essential for maintaining redox balance and sustaining cell proliferation.
LDHA’s nuclear localization has been observed in multiple cancer types, including colorectal cancer, breast cancer, prostate cancer, lung cancer, and liver cancer56 (reference to Human Protein Atlas available from www.proteinatlas.org), but the cause of LDHA nuclear translocation and the function of nuclear LDHA remains unclear. Our study discovered a ROS-dependent manner of LDHA nuclear translocation in response to HR-HPV infection, and α-HB produced by nuclear LDHA act as an important antioxidant metabolite facilitating cancer development. Given the high demand for ATP as “fuel” and metabolic intermediates as “building blocks” in cancer cells, aberrant proliferation generally accompanied with enhanced ROS production, suggesting that the mechanism of LDHA nuclear translocation is potentially ubiquitous. Our study provides a clue for how cancer cell balancing the demand of high proliferation rate and the high production of ROS: LDHA acts as a sensor for overloaded ROS, and then produce α-HB in the nucleus to enhance antioxidant capacity.
Nuclear pore complexes (NPCs) tightly control protein shuttling between cytoplasmic and nuclear. Protein is required to associate with importins or exportins to enter or exit the nucleus. Importins bind their cargo with NLS amino acid sequence in the cytoplasm, then interact with NPCs and pass through its channel. However, no classic NLS sequences were found in LDHA, suggesting the existence of noncanonical NLS signal or some unknown binding partners of LDHA facilitating its nuclear translocation.
Upon oxidative stress, an increased flux of cysteine into glutathione synthesis has been well-studied57. As a by-product of the methionine-to-glutathione pathway, α-HB production is directly linked to hepatic glutathione synthesis58. Our findings showed that α-HB level arises upon HPV16/18 E7 induction and cellular ROS accumulation in cervical cancer cells. Moreover, we speculate that the accumulation of α-HB could further enhance antioxidant responses that may produce more α-HB, presenting a positive feed-forward loop. Similarly, β-hydroxybutyrate (β-HB) was found to suppress cellular oxidative stress through inhibition of histone deacetylase59. We hypothesized if LDHA exerted the enzyme activity to catalyze β-ketobutyrate (β-KB) to β-HB. However, no such enzyme activity was detected under our tested experiment conditions. Recent studies also discovered another noncanonical enzyme activity of LDHA, catalyzing α-ketoglutarate (α-KG) to α-hydroxyglutarate (α-HG), under acidic pH conditions33,34. However, the activity was not detectable under our tested experiment conditions.
We uncovered the α-HB accumulation increases H3K79 methylation levels, which is DOT1L-dependent. Furthermore, an interaction of DOT1L and LDHA has been identified upon α-HB treatment (Fig. 4g). However, how DOT1L methyl-transferase activity is regulated via its binding with LDHA remains unknown, which is worth further investigation.
In conclusion, we demonstrate that HPV16-induced nuclear translocation of LDHA is sufficient to trigger antioxidant responses and activate Wnt pathway, leading to cell survival and proliferation under oxidative stress (Fig. 7g). As such, blocking LDHA nuclear translocation may offer more opportunities to cervical cancer prevention and ROS-based cancer therapies.
Antibodies and reagents
Antibodies against LDHA (Cell Signaling Technology, 3582, with 1:2500 working dilution for western blot and 1:500 working dilution for immunofluorescence; for mouse IHC, ABclonal, A1146, with 1:2000 working dilution), HPV16 E7 (Abcam, ab30731, with 1:2000 working dilution), Flag (Sigma, F3165, with 1:10000 working dilution for western blot and 1:1000 working dilution for immunofluorescence), Tubulin (Cell Signaling Technology, 3873, with 1:5000 working dilution), Lamin B1 (Cell Signaling Technology, 13435, with 1:2000 working dilution), H3K4me2 (Cell Signaling Technology, 9725, with 1:1000 working dilution), H3K9me2 (Cell Signaling Technology, 4658, with 1:1000 working dilution), H3K27me2 (Cell Signaling Technology, 9728, with 1:1000 working dilution), H3K36me2 (Cell Signaling Technology, 2901, with 1:1000 working dilution), H3K79me1 (Cell Signaling Technology, 12522, with 1:1000 working dilution), H3K79me2 (Cell Signaling Technology, 5427, with 1:1000 working dilution), H3K79me3 (Cell Signaling Technology, 4260, with 1:1000 working dilution; For IHC, Epigentek, A-4045, with 1:200 working dilution), H4K20me2 (Cell Signaling Technology, 9759, with 1:1000 working dilution), Histone H3 (Cell Signaling Technology, 4499, with 1:5000 working dilution), DOT1L (Abcam, ab72454, with 1:1000 working dilution), NRF2 (Abcam, ab62352, with 1:1000 working dilution), SOD1(Abcam, ab13498, with 1:2000 working dilution), MYC (Abcam, ab32072, with 1:2000 working dilution), β-actin (Invitrogen, MA5-15739, with 1:2000 working dilution) were purchased commercially.
Hydrogen peroxide solution (H2O2) (Sigma, 323381), N-acetyl cysteine (NAC) (Sigma, A7250), DAPI (Sigma, D9542), sodium pyruvate (Sigma, P5280), sodium (L) lactate solution (Sangon Biotech, A604046), sodium 2-ketobutyrate (Sigma, K0875), sodium 2-hydroxybutyrate (Santa Cruz, sc-258161), NADH (Sigma, N4505), EPZ004777 (Selleck, S7353), DPIC (Selleck, S8639), 50% glutaraldehyde solution (Sangon Biotech, A600875), and ML385 (MCE, HY-100523) were commercially obtained.
Cell culture and treatment
All the cell lines were purchased from American Type Culture Collection. HaCaT, HEK293T, SiHa, C33A, and HeLa cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco), and HT-3 and U2OS cells were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) in the presence of penicillin, streptomycin (Gibco) at 37 °C in humidified atmosphere containing 5% CO2.
Primary human cervix keratinocytes (PHKs) were isolated from normal cervical epithelial obtained from iCell (HUM-iCell-f016). PHKs were cultured in EpiLife Medium (Thermo Fisher, MEPI500CA) with the addition of EpiLife Defined Growth Supplement (EDGS) (Thermo Fisher, S0125). The Coating Matrix (Thermo Fisher, R011K) was used to enhance the attachment, growth, and population doubling potential of human keratinocytes. For all the experiments, keratinocytes cultured between the third and fifth passages were used.
For H2O2 treatment and NAC supplement, cells were plated in complete culture media with 10 μM H2O2 for 6 h, then exchanged the medium supplemented with 1 mM NAC for another 6 h.
Sample preparation, iodoTMT labeling, and mass spectrometry
5 × 106 cells cultured in media were washed in cold PBS, then lysed with lysis buffer (50 mM HEPES pH 7.9, 0.5 mM EDTA, 5 mM NaCl, 1% NP-40) adding 10 μl of LC/MS-grade methanol dissolved iodoTMT-126 reagent (Thermo Scientific, 90101) in an anoxic chamber. The lysates were sonicated for 30 s, then centrifuged at 16,000×g for 10 min at 4 °C to pellet insoluble materials. The supernatant was collected into a new tube and incubate at 900 rpm in ThermoMixer for 1 h at 37 °C protected from light. To remove the excessive iodoTMT-126 reagents, the proteins were precipitated with 500 μl of prechilled (−20 °C) acetone overnight at −20 °C. Precipitated proteins were washed twice with 500 μl of prechilled (−20 °C) acetone after centrifuged at 16,000×g for 30 min at 4 °C. The protein pellet was dissolved in 90 μl of denaturing alkylation buffer (6 M urea, 0.5% SDS, 10 mM EDTA, 200 mM Tris·HCl, pH 8.5) with 1 mM Tris (2-carboxyethyl) phosphine hydrochloride (TCEP), and adding 10 μl of LC/MS-grade methanol dissolved iodoTMT-127 reagent. The sample was incubated at 900 rpm in ThermoMixer for 1 h at 37 °C protected from light. Then, the iodoTMT-126/127-labeled protein was precipitated by prechilled (−20 °C) acetone as described.
For protein digestion, the precipitated proteins were dissolved in 100 μl 50 mM ammonium bicarbonate buffer, pH 8.0. Add 2.5 μl of trypsin to sample (1/50:trypsin/protein, w/w). Digest the sample overnight at 37 °C water bath. After digestion, peptides were cleaned up using C18 tips and lyophilized. For iodoTMT reagent enrichment, anti-TMT resin was used according to the manufacturer’s protocol.
For LC-MS/MS analysis, purified peptides were resuspended in 1% acetonitrile and 1% formic acid (FA) in H2O and analyzed by LC (Ultimate 3000, Thermo Scientific) equipped with self-packed capillary column (150 μm i.d. × 12 cm, 1.9 μm C18 reverse-phase fused-silica, Trap) using a 90 min nonlinear gradient at a flow rate of 600 nL/min. Eluted samples were analyzed by Q-Exactive HF (Thermo Fisher Scientific).
Tissue DNA extraction and HPV detection
The tissue DNA was extracted from 66 cervical cancer specimens using QIAamp DNA Mini Kit (Qiagen, 51304) following the manufacturer’s protocol. The tissues were mechanically disrupted and lysed with proteinase K at 56 °C overnight, and DNA was purified using QIAamp Mini spin column. PCR-reverse dot blot (PCR-RDB) assay were performed using HPV Genotyping Detection Kit (Yaneng Biotech, Shenzhen, China) according to the manufacturer’s instructions. The PCR reaction was amplified under the following conditions: an initial 50 °C for 15 min, 95 °C for 10 min; 40 cycles of 94 °C for 30 s, 42 °C for 90 s, and 72 °C for 30 s; and a final extension at 72 °C for 5 min. The PCR products were immobilized onto a nitrocellulose membrane and hybridized with typing probes. HPV subtypes were determined by the positive point on the HPV genotype profile on the membrane. HPV-positive and negative controls were also included in every experiment.
Human cervical tumor samples and immunohistochemistry (IHC)
Human cervical tumor samples were acquired from Sichuan Provincial People’s Hospital and West China Second Hospital. Informed consents were obtained from the patients. The human studies were approved by the ethic committee of Sichuan Provincial People’s Hospital and West China Second Hospital, Sichuan, China, and performed in compliance with the relevant ethical regulations. IHC staining was performed as previously described60.
To quantify the expression of LDHA, HPV16 E7, and H3K79me3, five random views were microscopically examined and analyzed by experienced pathologists. Images were captured using a charge-coupled device camera and analyzed using Motic Images Advanced software (version 3.2, Motic China Group). ImagePro Plus (version 6.0, Media Cybernetics) was used for further quantification of DAB intensity of the image cubes. The DAB signal of the cytoplasmic and nuclear LDHA or the nuclear HPV16 E7 and H3K79me3 were measured in this study. The mean value of HPV16 E7 signal intensity of each case was categorized into the corresponding groups by the following scores: 0 (negative staining); 1 (weak staining); 2 (moderate staining); and 3 (strong staining). To quantify the distribution of the nuclear LDHA and H3K79me3 signal intensities on four HPV16 E7 intensity groups, the mean value of each case was categorized into score 0 to 12. Further analysis was based on the IHC scores of LDHA, HPV16 E7, and H3K79me3.
Immunofluorescence and microscopy
Cells were plated at 1 × 106 per well on glass coverslips in 6-well culture plates and stimulated as described above. Cells were fixed with 4% paraformaldehyde for 15 min at room temperature, then rinsed twice in PBS. Permeabilization was performed with 0.2% Triton-X-100 in PBS for 30 min at room temperature. Primary antibody incubation was performed overnight in blocking buffer (0.5% BSA in PBS) at 4 °C. The following day, cells were washed twice in PBS, then incubated 4 h in secondary antibody in PBS at 4 °C. DAPI in PBS (5 mg/ml) was added prior to imaging. Microscopy was performed with OLYMPUS IX81 system. Image analysis was performed with the program CellSens Standard.
Quantification of cells with LDHA nuclear translocation
Nuclear translocation of LDHA was calculated as the percentage of total cells in each individual immunostained image. Five images were quantified for each group. Five hundred or more cells were counted for each condition. White indicates the overlay of LDHA (green), Flag (red), and DAPI (blue) suggesting the nuclear translocation of LDHA in HPV16 E7-expressing cells. Cyan indicates the overlay of LDHA (green) and DAPI (blue) suggesting the nuclear translocation of LDHA. Representative immunostaining images of LDHA nuclear translocation were shown in Fig. 1d–g, and Supplementary Figs. 2c, 3b–d.
Generation of stable cell pools
To generate stable LDHA knockdown HeLa cell pools, shRNA targeting LDHA was constructed and targeting sequences were as follows:
The retrovirus was produced by using a two-plasmid packaging system as previously described61. HeLa cells were infected with the retrovirus and selected with 2 μg/ml puromycin for 1 week. To generate LDHA knockdown and rescue stable cell pools, Flag-tagged human wild-type (WT), nuclear localization signal (NLS), or nuclear export signal (NES) LDHA was cloned into the retroviral pQCHIX vector and cotransfected with vectors expressing the gag and vsvg genes in HEK293T cells to produce retroviruses, HeLa cells with stable LDHA knockdown were then infected, following a selection with 200 μg/ml hygromycin B for 1 week. The NLS and NES sequences were listed as follow:
Generation of knockout cells
CRISPR-Cas9-based gene knockout was performed by oligonucleotides, three sgRNAs containing the LDHA-targeting sequences, and two sgRNAs containing NRF2-targeting sequences using lentiCRISPRv2 (Addgene) system. The targeting sequences were as follow:
LDHA sgRNA-1: 5′-CATTAAGATACTGATGGCAC-3′
LDHA sgRNA-2: 5′-CCGATTCCGTTACCTAATGG-3′
LDHA sgRNA-3: 5′-CCCGATTCCGTTACCTAATG-3′
NRF2 sgRNA-1: 5′-CCCGTCCCGGCACCACCGCA-3′
NRF2 sgRNA-2: 5′-TGGGACGGGAGTCCCGGCGG-3′.
Measurement of intracellular and mitochondrial ROS levels
ROS and mitochondrial superoxide production were determined using a fluorescent dye chloromethyl-2′,7′-dichlorofluorescein diacetate (H2DCF-DA, Sigma, 35845) and a specific mitochondrial superoxide indicator MitoSOX Red (Thermo Fisher, M36008), respectively. Briefly, cells with specified treatments were washed with PBS and incubated with 5 μM H2DCF-DA (or 10 μM MitoSOX) at 37 °C for 30 min to load the fluorescent dye. Afterward, cells were washed twice with PBS, and trypsinized, centrifuged, resuspended in 500 μl PBS. Cells were kept in the dark until analysis by flow cytometry (Accuri C6, BD Biosciences).
Isolation of nuclei was performed using the commercially available nuclei isolation kit: nuclei PURE prep from Sigma Aldrich (NUC201). Briefly, adherent cells were washed with PBS and scraped from the plate in the presence of lysis buffer. Cells (in lysis media) were carefully placed on top of a 1.8 M sucrose gradient and the resulting suspension was centrifuged at 30,000×g for 45 min in a precooled swinging bucket ultracentrifuge. Nuclei were collected as a white pellet at the bottom of the centrifuge and washed with nuclei storage buffer (provided within the kit). Isolated nuclei were immediately subjected to metabolite quantification.
LDHA enzyme activity assay
The canonical and noncanonical LDHA enzyme activity was determined as described previously62. For endogenous, cells with specified treatments were lysed by NP-40 buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.3% Nonidet P-40] containing protease inhibitors [1 mg/ml aprotinin, 1 mg/ml leupeptin, 1 mg/ml pepstatin, 1 mM Na3VO4, and 1 mM phenylmethylsulfonyl fluoride (PMSF)], and for exogenous, Flag-tagged WT/NLS/NES LDHA proteins were overexpressed in cells, immunoprecipitated with Flag-beads, eluted by Flag peptides (Gilson Biochemical), and subjected into activity assay with pyruvate and NADH as substrates for canonical enzyme activity and α-ketobutyrate (α-KB) and NADH as substrates for noncanonical enzyme activity, respectively. Reaction mixture consists of 50 mM Tris-HCl (pH 7.4), 20 μM NADH, 2 mM pyruvate (or 3.3 mM α-KB) in a total volume of 500 µL. Reactions were initiated by adding the enzyme and analyzed at 25 °C. Activities were measured by the conversion of NADH to NAD+, which was monitored by measuring the decrease of fluorescence (Ex. 350 nm, Em. 470 nm, HITACH F-4600 fluorescence spectrophotometer) for NADH decomposition. LDHA enzyme activities were normalized to LDHA protein level.
In total, 2 × 106 cells or nuclei were resuspended in 800 μl of ice-cold 80% methanol and 20% ddH2O. Samples were vigorously vortexed and placed in liquid N2 for 10 min to freeze. Then thawed on ice for 10 min, before the freeze-thaw cycle was repeated. Samples were centrifuged at 13,000 rpm to pellet cell debris, lipids and proteins. The supernatant was evaporated and resulting metabolites were resuspended in HPLC-grade H2O. Metabolites were normalized to protein concentration.
Metabolite analysis by LC-MS/MS
The metabolite samples were resuspended in 20 μl HPLC-grade H2O for mass spectrometry. Three microliter of sample extract was injected and analyzed using a 6500 Q-Trap triple quadrupole mass spectrometry (AB Sciex) coupled to a Prominence HPLC System (Shimadzu) via multiple reaction monitoring (MRM). Metabolites were targeted in negative ion mode (IonSpray Voltage: −4500V). Ultimate AQ-C18 column (1.7 μm, 2.1 × 250 mm, Welch) was used for separation of pyruvate, lactate, α-KB, and α-HB. Two gradient programs were applied: (1) for pyruvate and lactate. Mobile phase A: 0.01% formic acid in water; mobile phase B: acetonitrile-methanol (1:1); flow rate: 0.400 mL/min; gradient: starting from 0% phase B for 1.0 min, increased to 90% B from 1.0 to 4.0 min, 90% B was held from 4.0 to 5.0 min, 90% B to 0% B from 5.0 to 6.0 min, 0% B was held for 4.0 min to re-equilibrate the column. The stop time was 10.0 min. (2) For α-KB and α-HB. The mobile phase A, B, and flow rate were the same as 1). Gradients were run starting from 0% phase B for 1.0 min, increased to 60% B from 1.0 to 10.0 min, 60% B to 80% B from 10.0 to 11.0 min, 80% B was held from 11.0 to 13.0 min, 80% B to 0% B from 13.0 to 13.1 min, 0% B was held for 6.9 min to re-equilibrate the column. The stop time was 20.0 min. The other source parameters were kept constant as follows: curtain gas (CUR) was kept at 35 psi, ion source gas 1(GS1) was kept at 40 psi, ion source gas 2 (GS2) was kept at 40 psi, and the drying temperature was maintained at 600 ℃. Monitored transitions: pyruvate: m/z 87.0 > 43.0 [collision energy (CE): 10 V], lactate: m/z 89.0 > 43.0 (CE: 13 V), α-KB: m/z 101.0 > 57.0 (CE: 10 V), α-HB: m/z 103.0 > 57.0 (CE: 13 V). Peak areas from the total ion current for each metabolite were integrated using Skyline software (AB Sciex).
α-HB standard curve construction
The standard curve was prepared with seven concentrations of sodium α-hydroxybutyrate. The calibrators for sodium α-hydroxybutyrate was prepared at the following concentrations: 0.1, 0.5, 1.0, 5.0, 10.0, 25.0, 50.0 μM. The standard curve was determined using the linear regression of the peak area of sodium α-hydroxybutyrate against the interval standards. Quantification of the metabolite samples was calculated using interpolation.
Protein crosslinking assay
HaCaT and HT-3 cells treated with or without 10 μM H2O2 for 6 h and HEK293T LDHA KO cells expressing Flag-tagged WT, NLS, and NES LDHA were lysed by HEPES lysis buffer (40 mM HEPES pH 7.5, 150 mM NaCl, 0.1% NP-40) containing protease inhibitors for 30 min at 4 °C. Cell lysates were clarified by centrifuge at 16,000×g for 30 min at 4 °C. For protein crosslinking reactions, the supernatants were added with 0.025 wt. % glutaraldehyde for 30 min at 37 °C. The reactions were terminated by a final concentration of 50 mM Glycine. Samples were then separated by 8% SDS-PAGE and analyzed by western blotting with LDHA antibody.
Cells were treated with or without 10 μM H2O2 for 6 h and lysed by NP-40 buffer containing protease inhibitors (as above) for 30 min and centrifuged for 15 min at 13,000 rpm to remove cell debris. The gel filtration column (Superdex 200 Increase; GE Healthcare) was washed and equilibrated by cold PBS. Extracts were passed over the gel filtration column. The speed rate of flow is 0.4 ml/min. Fractions were collected every 0.25 ml per tube and analyzed by western blotting. Molecular mass was determined by Gel Filtration Calibration Kit HMW (GE Healthcare).
Coimmunoprecipitation and western blotting
Cells were lysed in ice-cold NP-40 buffer containing protease inhibitor (as above). The whole-cell lysates were incubated with protein A/G Sepharose beads (Roche) for 2 h at 4 °C and the beads were discarded to eliminate the non-specific binding. Then, the supernatants were incubated overnight with DOT1L antibody at 4 °C, followed by incubating with protein A/G Sepharose beads for another 2 h at 4 °C. Afterward, western blotting with indicated antibodies was performed. Standard western blotting protocols were adopted. Relative protein levels were quantified by measuring the band intensity of western blots by using Image J software. All the uncropped, full-size scans of western blots are presented in Supplementary Fig. 14.
Total RNA was isolated from cultured cells using Trizol reagent and reverse transcribed with random primers following the manufacturer′s instructions (TaKaRa). The cDNA was preceded to real-time PCR with gene-specific primers in the presence of SYBR Premix ExTaq (TaKaRa). PCR reactions were performed in triplicate on Roche LightCycler 480 System and the relative amount of cDNA was calculated by the comparative CT method using the β-actin as a control. Primer sequences were provided in Supplementary Data 3.
Chromatin immunoprecipitation (ChIP)-qPCR assays
ChIP-qPCR assays were performed as previously described60. Briefly, 2 × 106 HaCaT, HT-3, and HeLa cells were crosslinked with 1% paraformaldehyde, lysed and sonicated using the Bioruptor at high-output power setting for 40 cycles (30 s ON and 30 s OFF). Solubilized chromatin was immunoprecipitated with ChIP-grade antibody for H3K79me2 or rabbit IgG (negative control), following that the antibodies were preincubated with protein A sepharose beads overnight at 4 °C. Antibody-chromatin complexes were pulled down by protein A sepharose beads (Santa Cruz), washed with high salt buffer, LiCl buffer, TE buffer, and then eluted. After crosslink reversal in a water bath at 65 °C for 4 h and proteinase K digestion for 1 h at 55 °C, the ChIPed DNA was purified with QIAquick PCR Purification Kit (Qiagen, 28106). The DNA fragments were detected by qPCR. Histone H3K79 dimethylation marks were mapped at particular gene body regions of target genes (SOD1, CAT, CTNNB1, and MYC). Primers for the regions with peaks were designed for ChIP-qPCR analysis. All tested primers targeting SOD1, CAT, CTNNB1, and MYC are listed in Supplementary Data 3.
Cell proliferation assay and colony-formation assay
Cells were trypsinized, resuspended in PBS, and counted by using a hemocytometer. For cell proliferation assay, cells were plated in 6-well plates at 2500 cells per well in 2 ml media with or without H2O2, and plates were counted at 2, 4, 6, and 8 days. For colony-formation assay, cells were seeded in 6-well plates at 500 cells per well in 2 ml media with or without 10 μM H2O2. After 14 days, colonies were fixed in 4% paraformaldehyde and stained with 0.2% crystal violet. Colonies with >50 cells were counted. The media were exchanged every 24 h concerned about H2O2 decomposition.
The procedures related to animal subjects were approved by ethic committee of the Institutes of Biomedical Sciences (IBS), Fudan University, Shanghai, China. In total, 5 × 106 HeLa LDHA KO cells with Vec/WT/NLS/NES LDHA put back were subcutaneously injected into the flanks of athymic nude mice (6-week-old males): Vec on the left flank and WT on the right flank for mouse NO. 1–8, while NLS at the left flank and NES at the right flank for mouse NO. 1′–8′. Tumor volume was recorded by caliper measurements using the formula (length [mm]) × (width [mm]) × (height [mm]) × (π/6). Tumors were dissected and weighed at day 30, and tumor volumes derived from LDHA KO cells with Vec/WT/NLS/NES LDHA putting back were compared. All the animal studies were performed in compliance with the relevant ethical regulations.
TALEN targeting HPV16 E7 in K14-HPV16 transgenic mice
K14-HPV16 transgenic mice have been described previously63. Breeding pairs of K14-HPV16 transgenic mice were provided by the National Cancer Institute (NCI) Mouse Repository (Frederick, MD, USA) [strain nomenclature: FVB.Cg-Tg (KRT14-HPV16) wt1Dh] and bred at the Experimental Animal Center, HUST. HPV16-positive female mice (6 to 8 weeks old) were randomly grouped. The DNA of TALEN plasmid was mixed with TurboFect In Vivo Transfection Reagent (R0541, Fermentas, Thermo Scientific) according to the manufacturer’s protocol. Mice were anesthetized with 3% pentobarbital sodium (intraperitoneal injection of 50 mg/kg mice body weight), and the polymer–DNA complexes were pipetted into the vagina using 200 μl pipet tips after washing with saline (in a maximum volume of 20 μl)64. Then, mice were kept in the dorsal position for at least 40 min under anesthesia to avoid loss of the complexes. The mice were euthanized after pentobarbital anesthetization at the indicated times. The vagina was dissected, fixed in 4% paraformaldehyde, and sectioned for IHC. The protocol for the assembly of the TALEN targeting HPV16 E7 was described previously50. The TALEN target sequence was as follows: 5′-ATGTTAGATTTGCAACCAGAGACAACTgatctctactgttatgagc AATTAAATGACAGCTCAGAGGAGG-3′. Uppercase letters: left and right target sequences of TALEN, lowercase letters: spacer sequences.
Statistical analyses were performed with a two-tailed unpaired Student’s t-test. All data shown represent the results obtained from three (or as indicated) independent experiments with standard errors of the mean (mean ± SEM). The values of p < 0.05 were considered statistically significant. *, **, and *** denote p < 0.05, p < 0.01, and p < 0.001, respectively. NS means non significant.
The mass spectrometry proteomics data has been deposited to the ProteomeXchange Consortium via the PRoteomics IDEntifications (PRIDE) partner repository65 with the dataset identifier PXD011058. The datasets generated and analyzed during the current study are available within the article and the Supplementary Information files, and from the corresponding author upon reasonable request.
Ferlay, J. et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int. J. Cancer 136, E359–E386 (2015).
Schiffman, M. et al. Human papillomavirus testing in the prevention of cervical cancer. J. Natl. Cancer Inst. 103, 368–383 (2011).
Doorbar, J. Molecular biology of human papillomavirus infection and cervical cancer. Clin. Sci. (Lond.) 110, 525–541 (2006).
Stoler, M. H. et al. Human papillomavirus type 16 and 18 gene expression in cervical neoplasias. Hum. Pathol. 23, 117–128 (1992).
Mirabello, L. et al. HPV16 E7 genetic conservation is critical to carcinogenesis. Cell 170, 1164–1174 e1166 (2017).
Jabbar, S. F., Abrams, L., Glick, A. & Lambert, P. F. Persistence of high-grade cervical dysplasia and cervical cancer requires the continuous expression of the human papillomavirus type 16 E7 oncogene. Cancer Res. 69, 4407–4414 (2009).
Jabbar, S. F. et al. Cervical cancers require the continuous expression of the human papillomavirus type 16 E7 oncoprotein even in the presence of the viral E6 oncoprotein. Cancer Res. 72, 4008–4016 (2012).
Riley, R. R. et al. Dissection of human papillomavirus E6 and E7 function in transgenic mouse models of cervical carcinogenesis. Cancer Res. 63, 4862–4871 (2003).
Roman, A. & Munger, K. The papillomavirus E7 proteins. Virology 445, 138–168 (2013).
Cancer Genome Atlas Research, N. et al. Integrated genomic and molecular characterization of cervical cancer. Nature 543, 378–384 (2017).
Flores, E. R., Allen-Hoffmann, B. L., Lee, D. & Lambert, P. F. The human papillomavirus type 16 E7 oncogene is required for the productive stage of the viral life cycle. J. Virol. 74, 6622–6631 (2000).
Kamranvar, S. A. & Masucci, M. G. The Epstein-Barr virus nuclear antigen-1 promotes telomere dysfunction via induction of oxidative stress. Leukemia 25, 1017–1025 (2011).
Lin, W. et al. Hepatitis C virus regulates transforming growth factor beta1 production through the generation of reactive oxygen species in a nuclear factor kappaB-dependent manner. Gastroenterology 138, 2509–2518 (2010).
Yuan, K. et al. HBV-induced ROS accumulation promotes hepatocarcinogenesis through snail-mediated epigenetic silencing of SOCS3. Cell Death Differ. 23, 616–627 (2016).
Williams, V. M., Filippova, M., Filippov, V., Payne, K. J. & Duerksen-Hughes, P. Human papillomavirus type 16 E6* induces oxidative stress and DNA damage. J. Virol. 88, 6751–6761 (2014).
De Marco, F. Oxidative stress and HPV carcinogenesis. Viruses 5, 708–731 (2013).
Chen Wongworawat, Y., Filippova, M., Williams, V. M., Filippov, V. & Duerksen-Hughes, P. J. Chronic oxidative stress increases the integration frequency of foreign DNA and human papillomavirus 16 in human keratinocytes. Am. J. Cancer Res. 6, 764–780 (2016).
DeNicola, G. M. et al. Oncogene-induced Nrf2 transcription promotes ROS detoxification and tumorigenesis. Nature 475, 106–109 (2011).
Sporn, M. B. & Liby, K. T. NRF2 and cancer: the good, the bad and the importance of context. Nat. Rev. Cancer 12, 564–571 (2012).
Hanahan, D. & Weinberg, R. A. Hallmarks of cancer: the next generation. Cell 144, 646–674 (2011).
Warburg, O. On the origin of cancer cells. Science 123, 309–314 (1956).
Cortes-Malagon, E. M. et al. Gene expression profile regulated by the HPV16 E7 oncoprotein and estradiol in cervical tissue. Virology 447, 155–165 (2013).
Liu, Y. et al. Targeting hexokinase 2 inhibition promotes radiosensitization in HPV16 E7-induced cervical cancer and suppresses tumor growth. Int. J. Oncol. 50, 2011–2023 (2017).
Koukourakis, M. I., Giatromanolaki, A., Simopoulos, C., Polychronidis, A. & Sivridis, E. Lactate dehydrogenase 5 (LDH5) relates to up-regulated hypoxia inducible factor pathway and metastasis in colorectal cancer. Clin. Exp. Metastas-. 22, 25–30 (2005).
Zhang, R. et al. HPV E6/p53 mediated down-regulation of miR-34a inhibits Warburg effect through targeting LDHA in cervical cancer. Am. J. Cancer Res. 6, 312–320 (2016).
Saslow, D. et al. American cancer society, American society for colposcopy and cervical pathology, and American society for clinical pathology screening guidelines for the prevention and early detection of cervical cancer. CA Cancer J. Clin. 62, 147–172 (2012).
Benhar, M., Forrester, M. T. & Stamler, J. S. Protein denitrosylation: enzymatic mechanisms and cellular functions. Nat. Rev. Mol. Cell Biol. 10, 721–732 (2009).
Chen, C. A. et al. S-glutathionylation uncouples eNOS and regulates its cellular and vascular function. Nature 468, 1115–1118 (2010).
Lundquist, M. R. et al. Redox modification of nuclear actin by MICAL-2 regulates SRF signaling. Cell 156, 563–576 (2014).
Drummond, G. R., Selemidis, S., Griendling, K. K. & Sobey, C. G. Combating oxidative stress in vascular disease: NADPH oxidases as therapeutic targets. Nat. Rev. Drug. Discov. 10, 453–471 (2011).
Gorrini, C., Harris, I. S. & Mak, T. W. Modulation of oxidative stress as an anticancer strategy. Nat. Rev. Drug. Discov. 12, 931–947 (2013).
Xie, H. et al. Targeting lactate dehydrogenase–a inhibits tumorigenesis and tumor progression in mouse models of lung cancer and impacts tumor-initiating cells. Cell. Metab. 19, 795–809 (2014).
Intlekofer, A. M. et al. L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH. Nat. Chem. Biol. 13, 494–500 (2017).
Intlekofer, A. M. et al. Hypoxia induces production of L-2-hydroxyglutarate. Cell. Metab. 22, 304–311 (2015).
Rosalki, S. B. & Wilkinson, J. H. Reduction of alpha-ketobutyrate by human serum. Nature 188, 1110–1111 (1960).
Sutendra, G. et al. A nuclear pyruvate dehydrogenase complex is important for the generation of acetyl-CoA and histone acetylation. Cell 158, 84–97 (2014).
Tsang, C. K., Liu, Y., Thomas, J., Zhang, Y. & Zheng, X. F. Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance. Nat. Commun. 5, 3446 (2014).
Ghosh, R. et al. Allosteric inhibition of the IRE1alpha RNase preserves cell viability and function during endoplasmic reticulum stress. Cell 158, 534–548 (2014).
Um, J. W. et al. Alzheimer amyloid-beta oligomer bound to postsynaptic prion protein activates Fyn to impair neurons. Nat. Neurosci. 15, 1227–1235 (2012).
Gao, X., Wang, H., Yang, J. J., Liu, X. & Liu, Z. R. Pyruvate kinase M2 regulates gene transcription by acting as a protein kinase. Mol. Cell 45, 598–609 (2012).
Shyh-Chang, N. et al. Influence of threonine metabolism on S-adenosylmethionine and histone methylation. Science 339, 222–226 (2013).
Mentch, S. J. et al. Histone methylation dynamics and gene regulation occur through the sensing of one-carbon metabolism. Cell. Metab. 22, 861–873 (2015).
Daigle, S. R. et al. Selective killing of mixed lineage leukemia cells by a potent small-molecule DOT1L inhibitor. Cancer Cell. 20, 53–65 (2011).
Uren, A. et al. Activation of the canonical Wnt pathway during genital keratinocyte transformation: a model for cervical cancer progression. Cancer Res. 65, 6199–6206 (2005).
Ramachandran, I. et al. Wnt inhibitory factor 1 induces apoptosis and inhibits cervical cancer growth, invasion and angiogenesis in vivo. Oncogene 31, 2725–2737 (2012).
Mohan, M. et al. Linking H3K79 trimethylation to Wnt signaling through a novel Dot1-containing complex (DotCom). Genes Dev. 24, 574–589 (2010).
Ho, L. L. et al. DOT1L-mediated H3K79 methylation in chromatin is dispensable for Wnt pathway-specific and other intestinal epithelial functions. Mol. Cell. Biol. 33, 1735–1745 (2013).
Haddad, J. J. Antioxidant and prooxidant mechanisms in the regulation of redox(y)-sensitive transcription factors. Cell. Signal. 14, 879–897 (2002).
Singh, A. et al. Small molecule inhibitor of NRF2 selectively intervenes therapeutic resistance in KEAP1-deficient NSCLC tumors. Acs. Chem. Biol. 11, 3214–3225 (2016).
Hu, Z. et al. TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy. J. Clin. Invest. 125, 425–436 (2015).
Peng, M. et al. Aerobic glycolysis promotes T helper 1 cell differentiation through an epigenetic mechanism. Science 354, 481–484 (2016).
Zhao, D. et al. Lysine-5 acetylation negatively regulates lactate dehydrogenase A and is decreased in pancreatic cancer. Cancer Cell. 23, 464–476 (2013).
Brand, A. et al. LDHA-associated lactic acid production blunts tumor immunosurveillance by T and NK Cells. Cell. Metab. 24, 657–671 (2016).
Arseneault, R. et al. Attenuation of LDHA expression in cancer cells leads to redox-dependent alterations in cytoskeletal structure and cell migration. Cancer Lett. 338, 255–266 (2013).
Jin, L. et al. Phosphorylation-mediated activation of LDHA promotes cancer cell invasion and tumour metastasis. Oncogene 36, 3797–3806 (2017).
Uhlen, M. et al. Proteomics. Tissue-based map of the human proteome. Science 347, 1260419 (2015).
Cramer, S. L. et al. Systemic depletion of L-cyst(e)ine with cyst(e)inase increases reactive oxygen species and suppresses tumor growth. Nat. Med. 23, 120–127 (2017).
Lord, R. S. & Bralley, J. A. Clinical applications of urinary organic acids. Part I: detoxification markers. Altern. Med. Rev. 13, 205–215 (2008).
Shimazu, T. et al. Suppression of oxidative stress by beta-hydroxybutyrate, an endogenous histone deacetylase inhibitor. Science 339, 211–214 (2013).
Gao, X. et al. Acetate functions as an epigenetic metabolite to promote lipid synthesis under hypoxia. Nat. Commun. 7, 11960 (2016).
Wang, Y. P. et al. Arginine Methylation of MDH1 by CARM1 inhibits glutamine metabolism and suppresses pancreatic cancer. Mol. Cell 64, 673–687 (2016).
Plummer, D. T., Elliott, B. A., Cooke, K. B. & Wilkinson, J. H. Organ specificity and lactate-dehydrogenase activity. 1. The relative activities with pyruvate and 2-oxobutyrate of electrophoretically separated fractions. Biochem. J. 87, 416–422 (1963).
Arbeit, J. M., Munger, K., Howley, P. M. & Hanahan, D. Progressive squamous epithelial neoplasia in K14-human papillomavirus type 16 transgenic mice. J. Virol. 68, 4358–4368 (1994).
Palliser, D. et al. An siRNA-based microbicide protects mice from lethal herpes simplex virus 2 infection. Nature 439, 89–94 (2006).
Vizcaino, J. A. et al. The proteomics identifications (PRIDE) database and associated tools: status in 2013. Nucleic Acids Res. 41, D1063–D1069 (2013).
We thank the members of Fudan Cancer Metabolism laboratory for discussion throughout this study. We also thank the Biomedical Core Facility of Fudan University for their technical support. This work was supported by MOST (Grant No. 2015CB910401), NSFC (Grant No. 81790253, 81790251, 81821002, 81430057, 81430071, and 81672381) to Q.Y.L and C.H, Sichuan Science and Technology Program (Grant No. 2018RZ0133) to C.H, and Shanghai Municipal Education Commission (Grant No. N173606) to Q.Y.L.
The authors declare no competing interests.
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Liu, Y., Guo, JZ., Liu, Y. et al. Nuclear lactate dehydrogenase A senses ROS to produce α-hydroxybutyrate for HPV-induced cervical tumor growth. Nat Commun 9, 4429 (2018). https://doi.org/10.1038/s41467-018-06841-7
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