Myh10 deficiency leads to defective extracellular matrix remodeling and pulmonary disease

Kim, Hyun-Taek; Yin, Wenguang; Jin, Young-June; Panza, Paolo; Gunawan, Felix; Grohmann, Beate; Buettner, Carmen; Sokol, Anna M.; Preussner, Jens; Guenther, Stefan; Kostin, Sawa; Ruppert, Clemens; Bhagwat, Aditya M.; Ma, Xuefei; Graumann, Johannes; Looso, Mario; Guenther, Andreas; Adelstein, Robert S.; Offermanns, Stefan; Stainier, Didier Y. R.

doi:10.1038/s41467-018-06833-7

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Published: 02 November 2018

Myh10 deficiency leads to defective extracellular matrix remodeling and pulmonary disease

Nature Communications volume 9, Article number: 4600 (2018) Cite this article

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Abstract

Impaired alveolar formation and maintenance are features of many pulmonary diseases that are associated with significant morbidity and mortality. In a forward genetic screen for modulators of mouse lung development, we identified the non-muscle myosin II heavy chain gene, Myh10. Myh10 mutant pups exhibit cyanosis and respiratory distress, and die shortly after birth from differentiation defects in alveolar epithelium and mesenchyme. From omics analyses and follow up studies, we find decreased Thrombospondin expression accompanied with increased matrix metalloproteinase activity in both mutant lungs and cultured mutant fibroblasts, as well as disrupted extracellular matrix (ECM) remodeling. Loss of Myh10 specifically in mesenchymal cells results in ECM deposition defects and alveolar simplification. Notably, MYH10 expression is downregulated in the lung of emphysema patients. Altogether, our findings reveal critical roles for Myh10 in alveologenesis at least in part via the regulation of ECM remodeling, which may contribute to the pathogenesis of emphysema.

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Introduction

Lung development is subdivided into five chronologically and structurally distinct stages: embryonic, pseudoglandular, canalicular, saccular, and alveolarization^1,2. During the embryonic stage, the lung bud arises from the anterior foregut endoderm. From the pseudoglandular to canalicular stages, the lung undergoes branching morphogenesis to form a tree-like tubular structure. At the saccular stage, expansion and thinning of the alveolar walls lead to a marked decrease in interstitial tissue as a prerequisite for postnatal gas exchange. Finally, the lung greatly expands its alveolar surface by alveolarization, a process by which alveolar sacs are repeatedly partitioned through septation. Alveolar sac septation is a complex process, which requires interactions between lung epithelial and mesenchymal cells^3,4,5,6,7. Although several studies have focused on these epithelial–mesenchymal interactions, the underlying mechanisms are still poorly understood.

Non-muscle myosin II (NM II) plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division^8,9,10. NM II molecules consist of three peptide pairs: a pair of NM II heavy chains (NMHC II), a pair of regulatory light chains (RLCs), and a pair of essential light chains (ELCs). In mammals, three different genes, Myosin heavy chain 9 (MYH9), MYH10, and MYH14, encode the NMHC II proteins, NMHC IIA, NMHC II-B, and NMHC II-C, respectively^11,12. NM II proteins are highly expressed during lung development¹³, and their inhibition using blebbistatin leads to defects in branching morphogenesis and epithelial cell shape and orientation¹⁴. To date, however, genetic studies of NM II genes during lung development and homeostasis have not been conducted.

Many pulmonary diseases including chronic obstructive pulmonary disease (COPD)^15,16 and interstitial lung disease (ILD)¹⁷ are associated with significant morbidity and mortality due to impaired alveolar formation and maintenance. Emphysema is one form of COPD that results from the enzymatic destruction of lung extracellular matrix (ECM) components, including elastin and collagen, thereby leading to destruction of alveolar walls and airspace enlargement^15,16,18. Recently, several animal models have been established to study the pathophysiology of emphysema, including exposure to cigarette smoke and protease instillation, as well as genetic manipulation^18,19,20. The pathophysiological role of NM II in pulmonary emphysema, however, has not been investigated.

Here, starting with a forward genetic screen in mouse, we reveal an unexpected role for the NM II-associated actomyosin network in ECM remodeling during lung development and disease. Notably, our results from animal models and human patients suggest that alterations of the actomyosin network by loss of MYH10 function contribute to the pathogenesis of emphysema and may provide a promising target for preventive care of emphysema.

Results

Identification of lung mutants following ENU mutagenesis

To identify novel factors regulating early postnatal lung development in mouse, we carried out a forward genetic screen using N-ethyl-N-nitrosourea (ENU) mutagenesis²¹. From screening the F3 progeny of 170 G1 mice, we identified eight families exhibiting a recessive alveolar collapse phenotype. Mutant newborns from one of these families were cyanotic and exhibited respiratory distress with full penetrance (Fig. 1a, Supplementary Movie 1). As their lungs did not inflate, mutant pups died within 24 h after birth (Figs. 1b, c). Histological and morphometric analyses revealed reduced alveolar spaces and thick alveolar septal walls (Fig. 1d), along with dilated cardiomyopathy and hydrocephalus²² (Supplementary Fig. 1a, b). We observed no histological differences between wild-type and mutant littermate lungs at the pseudoglandular stage. However, from embryonic day (E) 17.5 onward, the mutant lungs displayed reduced saccular spaces and more abundant mesenchyme compared with wild-type siblings (Fig. 1e).

To investigate whether the increased tissue density observed in the mutant lungs resulted from increased cell proliferation, altered apoptosis, or differentiation defects, we performed immunostaining using two proliferation markers, proliferating cell nuclear antigen (PCNA) and phospho-histone 3 (pH3), and quantified positive cells from E17 to postnatal day 0 (P0). To differentiate between mesenchymal and epithelial cells, we co-stained for the epithelial cell markers NKX2-1 and E-cadherin (E-cad). Interestingly, both epithelial and mesenchymal cells exhibited increased cell proliferation in mutant lungs compared with wild-type siblings (Supplementary Fig. 1c, d, 2a, b), explaining at least in part the observed increase in tissue density. A minor increase in the number of apoptotic cells was also observed in mutant lungs compared with wild-type siblings (Supplementary Fig. 1c, 2c), suggesting that loss of mutant cells does not contribute in a major way to the observed phenotypes.

Next, we examined the differentiation status of mutant epithelial and mesenchymal cells by staining for several marker proteins. Interestingly, although the number of Vimentin-positive fibroblasts²³ was dramatically decreased in mutant lungs, unaltered nerve/glial antigen (NG) 2-positive pericyte-like cells²³ and endothelial cell numbers were observed in mutant lungs compared with wild-type siblings (Supplementary Fig. 1d). Moreover, mutant lungs exhibited an increase in the number of AT2 (alveolar epithelial type II) cells and AT2 cell marker (Sftpa, Sftpc, Abca3) expression levels (Supplementary Fig. 1e, 2d, e). Concurrently, we found a marked decrease in the expression levels of AT1 (alveolar epithelial type I) cell markers (homeodomin-only protein homeobox (HOPX), Podoplanin (PDPN), and receptor for advanced glycation end products (RAGE))²⁴, as well as of a mature AT2 cell marker (lysosome-associated membrane glycoprotein (LAMP) 3)²⁵ (Supplementary Fig. 1e, f, 2e).

Immature AT2 cells store glycogen, which is converted into surfactant in lamellar bodies as these cells differentiate²⁶. To examine the differentiation state of AT2 cells, we performed periodic acid–Schiff (PAS) staining, which revealed a substantial increase in the number of PAS-positive AT2 cells in P0 mutant lungs (Fig. 1f). By transmission electron microscopy (TEM), wild-type AT2 cells contained numerous lamellar bodies and apical microvilli at E18.5, whereas mutant AT2 cells displayed abundant glycogen, but fewer lamellar bodies and microvilli, indicating that they failed to differentiate (Fig. 1g). In addition, whereas AT1 cells in wild-type lungs covered the alveolar surface and exhibited a flattened morphology at E18.5 and P0, most AT1 cells in mutant lungs appeared densely packed and failed to flatten out, as observed by PDPN immunostaining (Fig. 1h). The numbers of distal airway epithelial cells including club cells and ciliated cells did not appear to be significantly altered between mutants and wild-type siblings (Supplementary Fig. 1g). Taken together, these results indicate that mutants exhibit disrupted lung epithelial and mesenchymal cell differentiation, resulting in abnormal AT1 cell morphology and defective saccular development.

Identification of a missense mutation in the Myh10 gene

To identify the causative mutation, we carried out whole-exome sequencing of mutant and wild-type siblings and identified a missense Leu-to-Arg mutation (c.T1373G;p.L458R) in the motor domain of NMHC II-B, which is encoded by the Myh10 gene (Figs. 2a, d). Next, we performed genetic linkage analysis by genotyping 102 G4 and G5 mutant mice and found complete association between the lung phenotype and the Myh10 mutation (Figs. 2b, c). We then performed a complementation test between the ENU-induced Myh10 mutant and a Myh10 null allele²². Trans-heterozygous mice exhibited the same lung and heart phenotypes as the Myh10 global^27,28 and ENU-induced mutants, indicating that the loss of Myh10 function is responsible for the lung phenotype (Supplementary Fig. 3a, b). In addition, the localization of MYH10^L458R was different than that of MYH10^WT in cultured fibroblasts: while MYH10^WT-Myc was localized as scattered punctae in stress fibers and lamellae²⁹, MYH10^L458R-Myc appeared dissociated from the actin bundles of stress fibers (Supplementary Fig. 3c). To quantify the expression levels of Myh10 mRNA and protein in the P0 mutant lungs, we used reverse transcriptase-quantitative PCR (RT-qPCR), western blotting, and immunostaining. Expression of Myh10 mRNA was not significantly altered in Myh10^L458R/L458R (hereafter Myh10^-/-) lungs compared with Myh10^+/+ and Myh10^+/L458R (hereafter Myh10^+/-) lungs. However, MYH10 protein expression was dramatically reduced in Myh10^-/- lungs compared with Myh10^+/+ and Myh10^+/- lungs (Supplementary Fig. 3d). These data suggest that possibly due to their inability to bind actin bundles, MYH10^L458R proteins are highly unstable and thus subsequently degraded. To test the expression pattern of Myh10 in the developing lung, we performed in situ hybridization and immunostaining. At E13.5, Myh10 transcripts were specifically detected in mesenchymal tissue in both whole-mount and cryosectioned lungs (Fig. 2e). At E16.5, Tbx4-positive mesenchymal cells³⁰ co-expressed MYH10, whereas E-cad-positive epithelial cells did not (Figs. 2f, g). At E18.5, MYH10 expression colocalized with α-smooth muscle actin (α-SMA) and platelet derived growth factor receptor-beta (PDGFR-β) expression in the lung mesenchyme, but not with NG2, VE-cadherin, or PDPN expression (Fig. 2g, Supplementary Fig. 3e). These results indicate that in developing lungs, Myh10 is specifically expressed in mesenchymal cells including myofibroblasts, lipofibroblasts, and smooth muscle cells but not in endothelial cells, pericyte-like cells, or epithelial cells.

Myh10 deficiency causes defects in ECM remodeling

To understand the molecular mechanisms of Myh10 function during lung development, we analyzed the transcriptome of Myh10^+/+ and Myh10^-/- lungs at the saccular stage. Many of the genes downregulated in Myh10^-/- lungs are known to participate in cell adhesion and contraction, as well as ECM remodeling (Supplementary Fig. 4a, Supplementary Data 1), prompting us to investigate the roles of NM II/MYH10 in this process. First, we examined the expression levels of several ECM components. Immunostaining and western blot data showed that expression of Fibronectin (FN), type I Collagen (COL I), and Tropoelastin, which form the interstitial ECM³¹, appeared significantly reduced in E18.5 Myh10^-/- lungs (Fig. 3a, Supplementary Fig. 4b). In contrast, expression of basement membrane (BM) ECM components³¹ type II (COL II) and type IV Collagen (COL IV), as well as that of Laminin, were not altered, suggesting that defects in MYH10 function affect the expression of proteins found in the interstitial ECM of alveoli but not of those in the alveolar BM (Supplementary Fig. 4d). However, RNA expression of FN, Col I, and Tropoelastin was unaltered in E18.5 Myh10^-/- lungs compared with wild-type lungs (Supplementary Fig. 4c). Using TEM, we analyzed ECM organization in the alveolar interstitium at E18.5. Mature elastic fibers were largely absent in the ECM of Myh10^-/- lungs, and elastic bundles appeared sparse and fragmented compared with the wild-type pattern (Fig. 3b). These data suggest that Myh10 deficiency disrupts expression levels and localization of ECM components, including FN, Col I, and Elastin. MYH10 is known to mediate primary cilia formation in a human retinal pigmented epithelial (RPE1) cell line^32,33. To test whether MYH10 regulates cilia formation in embryonic fibroblasts, we performed immunostaining for anti-acetylated α-tubulin to detect primary cilia. We found no obvious defects in cilia formation in Myh10-deficient fibroblasts compared with wild-type fibroblasts (Supplementary Fig. 4e). Moreover, expression of components of the Wnt, Sonic hedgehog (Shh), and fibroblast growth factor signaling pathways, which are involved in lung cell proliferation and differentiation^4,34, was not altered in Myh10^-/- lungs compared with wild-type siblings (Supplementary Fig. 4f), indicating that these signaling pathways are not responsible for the phenotypes observed in Myh10-deficient fibroblasts and lungs.

Excess MMP2 activity causes defective ECM remodeling

Metalloproteinases are a group of proteins essential for ECM remodeling, which includes members of the matrix metalloproteinase (MMP) and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family members^35,36,37. During embryonic development, MMP2 is one of the most highly expressed metalloproteinases in the lung³⁸. Through both MMP activity assays and western blotting, we found a dramatic increase of MMP2 activity in lysates of E18.5 Myh10^-/- lungs and of Myh10^-/- cultured fibroblasts compared with wild-type samples (Fig. 3c, Supplementary Fig. 5a, b). To test whether MMP inhibition could rescue the Myh10 mutant phenotypes, we first treated wild-type and Myh10 mutant embryonic fibroblasts³⁹ with GM6001, a broad-spectrum MMP inhibitor⁴⁰, and with the MMP2-specific inhibitor I⁴¹. Wild-type fibroblasts displayed well-organized, linear FN fibers; in contrast, only small aggregates of FN marked the cell surface of Myh10-deficient fibroblasts. Interestingly, the FN matrix defect was partially rescued by treatment with GM6001 or MMP2 inhibitor I (Fig. 3d, Supplementary Fig. 5c). Next, we administered GM6001 to pregnant females daily, covering a developmental time window from E16.5 to E18.5. This regimen was sufficient to partially rescue defective ECM remodeling in Myh10^-/- lungs (Fig. 3e). In addition, the FN matrix defect in Myh10^-/-fibroblasts was partially rescued by small interfering RNA (siRNA)-mediated Mmp2 knock-down (Figs. 3f, g). To investigate at which specific step Myh10 regulates ECM production, we assessed the expression of elastin assembly components in Myh10-deficient lungs. The expression of Fibrillin-1/Fbn1, Fibrillin-2/Fbn2, Fibulin-4/Fbln4, Fibulin-5/Fbln5, Lox, and Loxl1^42,43 was not significantly changed in E18.5 Myh10^-/- lungs compared with wild-type lungs (Supplementary Fig. 5d). Moreover, Myh10 deficiency did not cause defects in FN assembly in fibroblasts (Supplementary Fig. 5e), altogether suggesting that Myh10 does not directly control the expression, crosslinking, or assembly of ECM components in fibroblasts or developing lungs. Collectively, these results suggest that Myh10 deficiency leads to increased MMP activity, which disrupts ECM remodeling in fibroblasts in vitro and in developing lungs in vivo.

To understand how MYH10 regulates MMP activation, we performed proteomic profiling of wild-type and mutant fibroblasts and detected lower levels of Thrombospondins (THBS1 and THBS2) in Myh10^-/- fibroblasts (Figs. 4a, b, Supplementary Data 2). THBSs are matricellular proteins that interact with MMPs to inhibit their activities^44,45,46. Consistently, we found a dramatic decrease in THBS1 and THBS2 expression levels in cell lysates and conditioned media of mutant fibroblasts as well as in Myh10^-/- lungs (Fig. 4c, Supplementary Fig. 5f, g), further validating our proteomics results. Importantly, overexpression of Thbs2 partially rescued the FN matrix defect in Myh10^-/- fibroblasts (Fig. 4d), indicating that MYH10 regulates MMP activation through THBS.

Myh10 function is required in lung mesenchymal cells

To determine which cells require Myh10 function during mouse lung development, we utilized a floxed Myh10^fl/fl line⁴⁷. First, we deleted Myh10 expression in lung epithelial or endothelial cells by using Shh-Cre or Tek-CreER^T2, respectively, and found no obvious breathing dysfunction or lung defects (Supplementary Fig. 6a–g). To test Myh10 function in the lung mesenchyme, we crossed Gli1-CreER^T2 with Myh10^flox mice (hereafter Gli1-Myh10^cKO). By injecting tamoxifen into Gli1-Myh10^cKO;tdTomato mice at E13.5-E15.5, we found that Gli1-positive cells did not overlap with E-cad-expressing epithelial cells in P0 lungs (Fig. 5a). Gli1-Myh10^cKO newborn pups exhibited respiratory distress and cyanosis (Fig. 5b). Floating lung assays from wild-type and Gli1-Myh10^cKO newborns showed that the mutant lungs failed to inflate with air at birth (Figs. 5c, d). The expression of Myh10 mRNA and protein was dramatically decreased in Gli1-Myh10^cKO lungs as compared with wild-type and heterozygous littermates (Supplementary Fig. 7a, b). Gli1-Myh10^cKO mice also exhibited alveolar collapse, with no apparent cardiac defects (Fig. 5e, Supplementary Fig. 7c). Gli1-Myh10^cKO lungs exhibited an increased number of proliferating epithelial (NKX2-1/PCNA double positive) and mesenchymal (NKX2-1-negative/PCNA-positive) cells, as well as an increased number of AT2 cells (SFTPC-positive), as clearly in the ENU-induced allele (Figs. 5f, g). Finally, Gli1-Myh10^cKO lungs exhibited clearly decreased FN staining intensity, as well as truncated and disorganized elastin fibers (Fig. 5h). In summary, mesenchyme-specific loss of Myh10 led to lung defects that were observed in the global Myh10 knockout, as well as in the ENU-induced Myh10^L458R allele. These results indicate that Myh10 is specifically required in lung mesenchyme to promote ECM remodeling and cell differentiation.

Myh10 is required for lung alveologenesis

As MYH10 is still highly expressed in mesenchymal tissues throughout postnatal and adult stages (Supplementary Fig. 3e), we investigated Myh10 function during lung maturation and homeostasis. To test the role of Myh10 in alveologenesis, we deleted Myh10 in mesenchymal cells at early postnatal stages before secondary septae formation^1,2,5 (Supplementary Fig. 7d). By injecting tamoxifen into Gli1-Myh10^cKO;tdTomato mice at P0, we found that tdTomato-expressing cells did not overlap with E-cad-expressing epithelial cells in P7 lungs (Supplementary Fig. 7d–f). Gli1-Myh10^cKO mice displayed a simplified, dilated alveolar structure and shorter secondary septae compared with wild-type siblings (Figs. 6a, b). Furthermore, FN expression was significantly decreased, and elastin fibers appeared disorganized and truncated (Fig. 6c), suggesting that Myh10 is also required for secondary septation through ECM remodeling during postnatal lung development.

To test whether Myh10 participates in lung homeostasis, we deleted Myh10 in the mesenchymal tissue of adult mice (Supplementary Fig. 7g). Gli1-Myh10^cKO;tdTomato mice were injected with tamoxifen at 2 months, and tdTomato expression was detected in lung mesenchymal cells 1 month later, consistent with previous observations⁴⁸ (Supplementary Fig. 7g, h). At this stage, Gli1-Myh10^cKO mice exhibited disrupted septae and enlarged alveolar spaces, accompanied by macrophage accumulation in the alveolar interstitium and alveolar spaces (Fig. 6d and Supplementary Fig. 7i). Moreover, slightly increased numbers of apoptotic cells were observed in Gli1-Myh10^cKO lungs compared with wild-type siblings (Supplementary Fig. 7j). The expression of MMP2 was significantly increased in alveolar interstitial tissues, as well as in macrophages in Gli1-Myh10^cKO lungs compared with wild-type siblings (Fig. 6e), suggesting that upregulated MMP2 in the alveolar interstitium and in macrophages, as well an increased number of macrophages, might be involved in alveolar simplification of Myh10-deficient adult lungs. Whereas wild-type lungs displayed a well-organized elastin network, Gli1-Myh10^cKO lungs showed decreased elastin deposition and truncated and disorganized elastin fibers (Fig. 6e), suggesting that Myh10 continues to be required to maintain the elastin fiber network and alveolar structure during lung homeostasis.

MYH10 is downregulated in emphysematous lungs

As the conditional knockout phenotype in adult mice is histologically reminiscent of emphysema, we first measured the expression levels of MYH10 protein in human lung samples from healthy donors and emphysema patients. We found significantly downregulated MYH10 expression in lungs of emphysema patients when compared with healthy donor lungs (Figs. 7a, b). Next, we characterized MYH10-expressing cells in human lungs by immunostaining. Similar to MYH10 expression in mouse embryonic and adult lungs, MYH10-expressing human cells did not overlap with NKX2-1-expressing epithelial cells in the alveolar interstitium (Fig. 7c). MYH10 was strongly detected in smooth muscle cells surrounding blood vessels (Fig. 7d). We next analyzed MYH10 expression in human lung samples from 13 patients with emphysema and 7 healthy donors. In healthy lungs, MYH10 was expressed in smooth muscle cells and mesenchymal cells of the alveolar interstitium. In contrast, in emphysematous lungs, MYH10 levels were strongly reduced in smooth muscle cells and moderately reduced in the alveolar interstitium (Fig. 7e, Supplementary Fig. 8a, b). Overall, MYH10 was downregulated in 10 (77%) of the 13 emphysematous specimens (Supplementary Table 1). Taken together, these data indicate that MYH10 not only has a critical function in lung alveologenesis, but that it may also play a protective role against the occurrence of emphysema.

Discussion

Here, we identified a mutation in the mouse Myh10/NM II-B gene through an ENU mutagenesis screen and determined its role during lung development and alveologenesis, through a molecular mechanism involving MMP-mediated ECM remodeling. Myh10 mutant lungs display defects in: (1) ECM remodeling and cell proliferation during the canalicular stage, and (2) distal epithelial cell differentiation, AT1 cell morphogenesis, and interstitial remodeling/tissue morphogenesis during the saccular stage. As a consequence, mutant pups exhibit alveolar collapse and respiratory distress, and die shortly after birth (Supplementary Fig. 9a).

Our results implicate Myh10 in the maintenance of lung ECM components. The ECM is a three-dimensional, dynamic structure that is constantly remodeled to control tissue homeostasis. Dynamic remodeling of the ECM is essential for organ development and homeostasis and its dysregulation can result in pathological conditions. Thus, proteins involved in ECM degradation and modification, including MMPs, are essential for metazoan development^35,36. ECM-remodeling MMPs are tightly regulated at the transcriptional, post-transcriptional, and post-translational levels. Examples of biological processes regulating MMP expression and/or function include mechanical forces and actomyosin contractility. Based on our mass spectrometry-based proteomic analysis, we propose a Myosin-dependent regulation of MMP2, which involves another ECM molecule, THBS. Secretion of THBSs and their distribution in the ECM has been shown to be regulated by the actin cytoskeleton. Elevated ECM deposition of THBS1 or THBS2 is associated with increased tissue stiffness, such as in fibrosis and atherosclerotic plaques^49,50. Interestingly, in these contexts, intracellular tensile forces (contractility) are also increased⁵⁰, suggesting a reciprocal system by which THBS can modulate the actomyosin network. Our results implicate Myh10 in the maintenance of lung ECM components. The structural actomyosin network defects caused by the loss of Myh10^51,52 is likely to inhibit the secretion of THBS, in turn leading to de-repression of MMP activity, and disrupted ECM remodeling (Supplementary Fig. 9b). Thus, we propose a signaling axis controlling ECM homeostasis through the actomyosin network, THBSs and MMPs. Although our data point to a critical role for MYH10/THBS/MMP signaling in ECM remodeling during lung development and homeostasis, how the actomyosin network regulates extracellular deposition of THBS via MYH10 function will require further investigation.

Our study also uncovers a potential link between loss of MYH10 and COPD. COPD is a common disease worldwide, associated with high morbidity, and characterized by progressive airflow obstruction that is poorly reversible, airway inflammation, and systemic effects^15,16. The main risk factor for the development of COPD is smoking, but other factors such as air pollutants and genetic determinants have been identified. Pulmonary emphysema, a key symptom of COPD, results from the enzymatic destruction of lung ECM components including elastin and collagen, thereby decreasing tissue stiffness and stability^53,54,55. Tissue stiffness has been shown to induce actin cytoskeleton reorganization and contractility, which in turn induces matrix rigidity, and modulates cell adhesion, migration, division, and differentiation^56,57. In our study, we observed that downregulation of MYH10 expression in human emphysema correlates with disease progression, and Myh10-deficient mice display emphysematous lungs, supporting the notion that Myh10 deficiency might lead to progressive destruction of the lung mesenchyme. Moreover, two genome-wide studies have reported mutations and reduced expression of two actomyosin-related genes, Myosin ID (MYO1D)⁵⁸ and Myosin Light Chain Kinase (MYLK)⁵⁹ in emphysematous human lungs, thus strengthening the relevance of our model.

Excess MMP activity in lungs is known to be associated with pulmonary diseases including COPD, acute respiratory distress syndrome, sarcoidosis, and tuberculosis^60,61. In COPD, MMPs are upregulated and their inhibition prevents disease progression in animal models^62,63. In the present study of Myh10 deficiency during lung development and homeostasis, excess MMP2 activity and consequent defective ECM remodeling partially recapitulate the pathophysiological features of human COPD, especially emphysema. Although mutations in the MYH10 locus have not yet been implicated in the pathogenesis of COPD, further studies are required to investigate possible correlations between NM II/MYH10 and COPD pathology. Our results from animal models and human patients suggest that structural alterations of the actomyosin network by loss of Myh10 function contribute to the pathogenesis of emphysema. We propose MYH10 as a diagnostic tool for COPD and introduce a mouse model to enable further investigation into the etiology of, and therapeutic approaches for, this disease.

Methods

Mouse ENU mutagenesis

ENU-injected C57BL/6 males were obtained from Dr. Monica Justice (Baylor College of Medicine, Houston, TX) ⁶⁴ and crossed with C57BL/6 females to generate G1 males (N = 170). G1 males were subsequently crossed with C57BL/6 females to generate G2 females, which were backcrossed to G1 males to generate G3 pups. G3 litters were screened for lung and tracheal malformations from P0 to P7⁶⁵. Genomic DNA from mutant mice and their littermates was isolated for whole-exome sequencing.

Mouse strains

The following mouse lines were kindly provided for our study: Myh10-flox by Dr. Robert Adelstein; Shh-Cre and Gli1-CreER^T2;ROSA26^tdTomato by Dr. Saverio Bellusci; Tek-CreER^T2 by Dr. Stefan Offermanns; and CMV-Cre by Dr. Thomas Braun. Tamoxifen (Sigma) was dissolved in corn oil at 50 mg/ml and intraperitoneally injected at the indicated developmental stages and frequencies. GM6001 (TOCRIS, catalog no.: 2983) was dissolved in dimethylsulfoxide (DMSO), and fresh dilutions in saline to a total volume of 200 μl were prepared immediately before administration to pregnant females. Intraperitoneal injection of GM6001 (100 mg/kg) was performed once daily from E16.5 and lung tissues were collected from E18.5 embryos (Fig. 3e). All animal care and use procedures in this study were approved by the local animal ethics committee (Regierungspräsidium Darmstadt, Hessen, Germany). All animal studies have been carried out in adherence to the relevant ethical guidelines.

Whole-exome sequencing and genotyping

For whole-exome sequencing, genomic DNA samples were extracted from two wild-type and two mutant pups according to standard protocols. The whole-exome sequencing was performed by the Beijing Genomics Institute (BGI). Genomic DNA was captured using Agilent SureSelect Biotinylated RNA Library (BAITS) and loaded on a HiSeq2000 platform for sequencing. Sequence reads were aligned to the C57BL/6J mouse reference genome (mm10) and analyzed using CLCBio Genomic Workbench and GATK software. Single-nucleotide polymorphisms (SNPs) were called using the GATK haplotype caller, closely following the GATK best practices. Briefly, aligned reads were de-duplicated and re-aligned to all exonic sequences from the Gencode genome annotation (version vM4), taking into account known variants from DBsnp. After base re-calibration, SNP calling was performed within exons, allowing a padding of 100 bp into flanking introns. Variant calls were annotated to known SNPs from DBsnp (version 142) and the functional relevance of variant calls was predicted using Annovar. Next, calls from wild-type mice were merged (unified), while calls from mutant mice were intersected. Intersecting calls were classified as mutant/homozygous if the corresponding wild-type call was heterozygous/absent or as mutant/heterozygous if there was no corresponding wild-type call. For genotyping, an Eco Real-Time PCR System (Illumina) was used for PCR reactions and high-resolution melt analysis. PCR reaction conditions were as follows: 95 °C for 15 s, 40 cycles of 95 °C for 2 s, 60 °C for 2 s, and 72 °C for 2 s. Following PCR, a high-resolution melt curve was generated by collecting SYBR-green fluorescence data in the 65–95 °C range. The analyses were performed on normalized derivative plots. The following primers were used: Myh10 (exon 12) forward 5ʹ-TGGATAGGACCAAACGCCAG-3ʹ and Myh10 (exon 12) reverse 5ʹ-GGAGAGAGTTGAGGAATACCTC-3ʹ.

Plasmids

Total RNA and complementary DNA (cDNA) were obtained from E13.5 lungs using TRIzol reagent (Life Technologies) and Superscript III reverse transcriptase (Life Technologies), respectively, according to manufacturer’s instructions. cDNA encoding the MYH10 (NCBI Acc. No. NM_175260) wild-type and MYH10^L458R proteins was PCR amplified using total cDNA from E13.5 lungs as a template. PCR fragments were cloned into the pcDNA3.1-Myc-His vector at the HindIII site using the Cold Fusion Cloning Kit (System Biosciences). Thbs2 (NCBI Acc. No. NM_011581) cDNA was PCR amplified using total cDNA from E18.5 lungs as a template. PCR fragments were ligated to the pcDNA3.1-Myc-His vector at XhoI/EcoRI sites. The following primers were used: Myh10 forward 5ʹ-TCCGAGCTCGGTACCACTGTTTACAATGGCCCAGAG-3ʹ and Myh10 reverse 5ʹ-TTGTTCGGGCCCAAGCTCTGATTGGGGTGGCTGTG-3ʹ; Thbs2 forward 5ʹ-AGGTCGACGTCACAGGTGGAGACAAGATG-3ʹ and Thbs2 reverse 5ʹ-TGGAATTCTCGAGGCATCTCTGCACTCA-3ʹ. To synthesize RNA probes for in situ hybridization, cDNA was PCR amplified using total cDNA from E13.5 lungs as a template. PCR fragments were cloned into the pGEM T-easy vector (Promega). The following primers were used: Myh10 forward 5ʹ-GTACAGAAAGCCCAGACCAAAGA-3ʹ and Myh10 reverse 5ʹ-TTCTTCATCAGCCACTCATCTGC-3ʹ; Tbx4 forward 5ʹ-CTTCTACCACTGCCTGAAGCGT-3ʹ and Tbx4 reverse 5ʹ-AGTCTCGTCATCCATCGGTCCA-3ʹ.

In situ hybridization and immunohistochemistry

In situ hybridization was performed according to standard procedures. Digoxigenin-labeled RNA probes for Myh10 and Tbx4 (NCBI Acc. No. NM_011536) were used. Hybridization and probe washes were performed at 70 °C. Immunohistochemistry was performed according to a standard protocol. The following antibodies and dilutions were used for immunostaining: acetylated α-tubulin (Sigma, 1:5000), CGRP (Abcam, 1:5000), cleaved caspase 3 (Cell Signaling, 1:500), c-Myc (Santa Cruz Biotechnology, 1:500), Col I (Abcam, 1:500), Col II (Santa Cruz Biotechnology, 1:100), Col IV (Abcam, 1:500), E-cad (Santa Cruz Biotechnology, 1:500), FN (Abcam, 1:1000), HOPX (Santa Cruz Biotechnology, 1:200), Isolectin B4, FITC-conjugate/IsoB4 (Sigma, 1:500), Laminin (Sigma, 1:1000), LAMP3 (1:100, Imgenex), MMP2 (Millipore, 1:500), MYC (Santa Cruz Biotechnology (9E10), 1:1000), MYH10 (Sigma, 1:500), NG2 (Millipore, 1:500), NKX2-1 (Santa Cruz Biotechnology, 1:200), PCNA (DAKO, 1:200), PDGFR-β (R&D Systems, 1ː200), PDPN/T1α (Developmental Studies Hybridoma Bank, 1:20), PECAM/CD31 (Developmental Studies Hybridoma Bank, 1:1), phalloidin, rhodamine-conjugate (ThermoFisher, 1:1000), phospho-histone H3 (Cell Signaling, 1:1000), RAGE (R&D Systems, 1:200), SCGB1A1/CC10 (Millipore, 1:1000), SFTPC/ProSP-C (Millipore, 1:1000), α-SMA (Sigma, 1:1000), THBS1 (Santa Cruz Biotechnology, 1:200), THBS2 (Santa Cruz Biotechnology, 1:200), tropoelastin (Abcam, 1:1000), and VE-cadherin (BD Biosciences, 1:500).

RT-qPCR and western blot analysis

Total RNA and cDNA were obtained from fetal and postnatal lungs using TRIzol reagent (Invitrogen) and Superscript III reverse transcriptase (Invitrogen), respectively, according to the manufacturer’s instructions. For quantitative reverse transcription PCR, the CFX Connect Real-Time system (Bio-Rad) and DyNAmo colorFlash SYBR green qPCR kit (ThermoFisher Scientific) were used. The primer sequences used for qPCR are shown in Supplementary Table 2. The Ct values are shown in Supplementary Table 3. For western blots, protein extracts were prepared from fetal and postnatal lungs by using NP-40 buffer (150 mM NaCl, 1.0 % NP-40, 50 mM Tris pH 8.0, 1 mM phenylmethane sulfonyl fluoride). Western blotting was performed according to standard protocols. Primary antibodies were incubated overnight at 4 °C in 3% skim milk. The following antibodies were used: α-actin (Sigma, 1:2000), Col I (1:2000), GAPDH (Cell Signaling, 1:2000), FN (1:2000), MMP2 (Millipore, 1:2000), MMP9 (Millipore, 1:2000), MT1-MMP/MMP14 (Santa Cruz Biotechnology, 1:500), MYH10 (1:2000), THBS2 (1:500), and tropoelastin (1:2000). All uncropped images related to western blotting data are available in Supplementary Fig. 10.

Histology and head skeletal staining

Whole lungs were fixed in 4% formaldehyde, embedded in paraffin, and sectioned at a thickness of 5 μm. For cryosections, fixed tissues were embedded in optimal cutting temperature (OCT) compound and 10 μm thick coronal sections were collected. Hematoxylin and eosin (H&E) and PAS staining were performed according to standard protocols. For head skeletal staining, newborn mice were deskinned, dehydrated in 95% ethanol for 1 day and acetone for 1 day. Skeletons were stained with 0.03% alcian blue (Sigma) and 0.005% Alizarin red (Sigma). After staining, samples were cleared in 2% potassium hydroxide and transferred to 50% glycerol for 2 days.

TEM

For TEM analysis, tissues were fixed in 3% glutaraldehyde, followed by 4% osmium tetraoxide (OsO₄) in 0.1 mM sodium cacodylate buffer (pH 7.4). After dehydration in ethanol and propylene oxide, the samples were embedded in Epon 812. Ultrathin sections were cut with a diamond knife and stained with uranyl acetate and lead citrate, and examined with a JEM1400 electron microscope (JEOL).

Cell culture, chemical treatments, and transfections

NIH3T3 cells and Myh10 null mouse embryonic fibroblasts were provided by Dr. Stefan Offermanns and Dr. Robert S. Adelstein, respectively. Embryonic fibroblasts, and NIH3T3 cells were maintained at 37 °C in a 5% CO₂ incubator in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10% fetal bovine serum, 50 g/ml streptomycin, 50 U/ml penicillin, and 250 ng/ml amphotericin B. Cells were treated with DMSO, Blebbistatin (10 μM), GM6001 (10 μM), and MMP2 inhibitor I (Santa Cruz Biotechnology, SC-204092, 10 μM) at 37 °C in a 5% CO₂ incubator for 24–48 h. Transfection was performed using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific) according to manufacturer’s instructions.

MMP activity assays

For gelatin zymography, lung tissues were homogenized in homogenization buffer (50 mM Tris-HCl, 0.5% Triton X-100, pH 7.4) and supernatant containing gelatinases was collected following centrifugation. For the MMP activity assay, a standard commercial assay kit (Abcam) was used to detect the general activity of MMPs in Myh10 null and wild-type mouse embryonic fibroblasts according to manufacturer’s instructions. Cells were cultured at 37 °C in a 5% CO₂ incubator in serum-free DMEM for 48 h. Cell lysates and conditioned media were incubated with a fluorescent substrate and fluorescence was measured using a fluorescence microplate reader (FLUOstar Omega, BMG LABTECH).

siRNA knockdowns

siRNAs for EGFP (EHUEGFP, Sigma) and Mmp2 (EMU04861, Sigma) were transfected using RNAiMAX transfection reagent (13778, Life Technologies) according to the manufacturer’s instructions.

FN assembly assay

Myh10 null and wild-type mouse embryonic fibroblasts were cultured for 24 h in complete growth medium. Cells were then kept for 24 h in starvation medium and scraped into 2% Deoxycholate (DOC) lysis buffer. DOC-soluble material was removed, and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer was added to these lysates. DOC-insoluble material was resuspended in solubilization buffer and treated with SDS-PAGE sample buffer. All samples were boiled for 5 min before western blot analysis.

Transcriptomic analysis (RNAseq)

For RNAseq, total RNA was isolated from E17 lungs (left lobes) of two Myh10^+/+ and two Myh10^-/- mice using Trizol (Life Technologies) combined with on-column DNase digestion (TURBO DNAse, Ambion) to avoid contamination by genomic DNA. RNA and library preparation integrity were verified with a BioAnalyzer 2100 (Agilent) or LabChip Gx Touch 24 (Perkin Elmer). In all, 3 µg of total RNA was used as input for Truseq Stranded mRNA Library preparation following the low sample protocol (Illumina). Sequencing was performed on a NextSeq500 instrument (Illumina) using v2 chemistry, resulting in 30 M–50 M reads per library, with 1 × 75 bp single-end setup. The resulting raw reads were assessed for quality, adapter content, and duplication rates with FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc). Reaper version 15-065 was employed to trim reads after a quality drop below a mean of Q20 in a window of 20 nucleotides. Only reads between 15 and 75 nucleotides were considered for further analyses. Trimmed and filtered reads were aligned to the Ensembl mouse genome version mm10 (GRCm38) using STAR 2.4.0a with the parameter “--outFilterMismatchNoverLmax 0.1” to increase the maximum ratio of mismatches to mapped length to 10%. The number of reads aligning to genes was counted with the featureCounts 1.5.1 tool from the Subread package. Only reads mapping at least partially inside exons were admitted and aggregated per gene. Reads overlapping multiple genes or aligning to multiple regions were excluded. Differentially expressed genes (DEGs) were identified using DESeq2 version 1.14.1. Only genes with a minimum fold change of ± 2 (log2 ± 1), a maximum Benjamini–Hochberg corrected P-value of 0.05, and a minimum combined mean of five reads were considered to be significantly differentially expressed. The Ensembl annotation was enriched with UniProt data based on Ensembl gene identifiers (Activities at the Universal Protein Resource (UniProt)). To examine the biological significance of the DEGs, we carried out a Gene Ontology (GO) enrichment analysis using the online tool Database for Annotation, Visualization and Integrated Discovery (DAVID) Bioinformatics Resource v6.8 (https://david.ncifcrf.gov/home.jsp)⁶⁶ (Supplementary Data 1).

Proteomic analysis (mass spectrometry)

Triplicate analyses were performed as follows: Sample preparation. Cells were lysed by heating to 70 °C for 10 min in SDS buffer (4% SDS in 0.1 M Tris/HCl, pH 7.6). DNA was sheared by sonication and cell debris was removed by centrifugation at 16,000 g for 10 min. A colorimetric 660 nm protein assay (Pierce) was used to determine the concentration of solubilized proteins in the resulting supernatants. Proteins were subsequently precipitated by addition of four volumes of acetone and incubation at –20 °C overnight, followed by pelleting at 14,000 g for 10 min and washing the pellet with 90% acetone. Samples were dried to remove acetone completely and dissolved in urea buffer (6 M urea, 2 M thiourea, 10 mM HEPES, pH 8.0). Enzymatic digestion of proteins was performed by in-solution digestion. Briefly, protein disulfide bonds were reduced with 4 mM dithiothreitol and alkylated with 20 mM iodoacetamide. Proteins were then cleaved using Lys-C (50:1 protein-to-enzyme ratio) (Wako Chemicals GmbH) at room temperature for 3 h, followed by overnight trypsination (50:1 protein-to-enzyme ratio) (Serva) at room temperature. Peptide labeling by reductive dimethylation was performed as previously described⁶⁷. Peptide concentration in trypsin-digested samples was measured using the Fluorimetric Peptide Assay (Pierce). Samples containing equal amounts of peptides (75 µg) were subjected to the dimethyl (in-solution) labeling protocol. In brief, peptide N-termini and lysine residues were methylated for 1 h at RT by formaldehyde-H₂ and cyanoborohydride (light, control mouse fibroblasts) and formaldehyde-¹³C–D₂ and cyanoborodeuteride (heavy, Myh10-KD), respectively (all reagents: Sigma). The reaction was quenched by acidification and differentially labeled samples were mixed in 1:1 ratio. Mixed samples were fractionated using the high pH reversed-phase peptide fractionation kit (Pierce) according to manufacturer’s instructions.

Liquid chromatography/tandem mass spectrometry. For mass spectrometry analysis, fractionated peptides were reconstituted in 10 µl of solvent A (0.1% formic acid). Peptides were separated using an UHPLC system (EASY-nLC 1000, ThermoFisher Scientific) and 20 cm in-house packed C18 silica columns (1.9 µm C18 beads, Dr. Maisch GmbH) coupled in line to a QExactive HF orbitrap mass spectrometer (ThermoFisher Scientific) using an electrospray ionization source. The gradient employed used linearly increasing concentrations of solvent B (90% acetonitrile, 1% formic acid) over solvent A (5% acetonitrile, 1% formic acid) from 5 to 30% over 215 min, from 30 to 60%, from 60 to 95% and from 95 to 5% for 5 min each, followed by re-equilibration with 5% of solvent B. The flow rate was set to a constant 400 nl/min. Full MS spectra were acquired for a mass range of 300–1750 m/z with a resolution of 60,000 at 200 m/z. The ion injection target was set to 3 × 10⁶ and the maximum injection time limited to 20 ms. Ions were fragmented by higher energy collision dissociation using a normalized collision energy of 27, an isolation window width of 2.2 m/z and an ion injection target of 5 × 10⁵ with a maximum injection time of 20 ms. Precursors with unassigned charge state and a charge state of 1 were excluded from selection for fragmentation. The duration of dynamic exclusion was set to 20 s. Resulting tandem mass spectra (MS/MS) were acquired with a resolution of 15,000 at 200 m/z using data dependent mode with a loop count of 15 (top15).

Data analysis. MS raw data were processed by MaxQuant (1.5.6.5) using the Uniprot mouse database (as of 20 April 2017) containing 89,316 entries and the following parameters: a maximum of two missed cleavages, mass tolerance of 4.5 ppm for the main search, trypsin as the digesting enzyme, carbamidomethylation of cysteins as a fixed modification, and oxidation of methionine, as well as acetylation of the protein N-terminus as variable modifications. For protein quantification based on dimethyl labeling, isotope labels were configured for peptide N-termini and lysine residues with a monoisotopic mass increase of 28.0313 and 36.0757 Da for the light and heavy labels, respectively. Peptides with a minimum of seven amino acids and at least one unique peptide were included in protein identification. MaxQuant was instructed to filter for 1% false discovery rate on both the peptide and protein levels. Only proteins with at least two peptides and one unique peptide were considered as identified and were used for further data analysis. The resulting data contained 2949 protein groups and was subjected to differential expression analysis using the in-house R package autonomics (https://bitbucket.org/graumannlabtools/autonomics; version 1.0.21), which makes heavy use of functionality provided by the limma package⁶⁸. The analysis considered protein groups that were quantified in at least one of the triplicate samples, with lack of completeness strongly penalized by the underlying Bayesian-moderated t-testing. To examine the biological significance of the differentially expressed proteins, we used autonomics to carry out overrepresentation analysis for GO terms using a Fisher's exact test (Supplementary Data 2).

Human emphysematous lung samples

Emphysematous lungs and healthy control lungs were provided by the UGMLC Giessen Biobank, which is a member of the DZL Platform Biobanking. All patients were diagnosed with COPD/emphysema by pulmonary function testing, computed tomography scans, and pathological examination from resected lung specimens. The severity of COPD was classified according to the Global Initiative on Obstructive Lung Disease (GOLD) staging system and all subjects belonged to GOLD IV. The severity of emphysema was assessed by pathological inspection of histological samples and graded according to the method as previously described⁶⁹. The study protocol was approved by the Ethics Committee of the Justus-Liebig-University School of Medicine (no. 58/2015), and informed consent was obtained in written form from each patient. All human studies were performed in adherence to the relevant ethical guidelines.

Morphometric analyses

Pictures of H&E-stained distal lungs were acquired using a Zeiss widefield microscope (AxioImager). Alveolar spaces, thickness of alveolar walls, and length of secondary septae were measured using ImageJ (https://imagej.nih.gov/ij/) according to the American Thoracic Society guidelines⁷⁰. A minimum of five representative, non-overlapping fields of view from lungs of at least three mice from each group were evaluated.

Statistical analysis

Data are presented as mean ± s.e.m of at least three biological replicates. Two-tailed Student’s t-tests were used to assess significance. A P-value of < 0.05 was considered significant.

Data availability

RNA-sequencing data from this study have been deposited in GEO under accession code GSE119399 (Supplementary Data 1). Proteomic analysis data from this study have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD011139 (Supplementary Data 2). All remaining data will be available from the corresponding author upon reasonable request.

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Acknowledgements

We thank Saverio Bellusci for the Shh-Cre and Gli1-CreER^T2 mice, Nina Wettschureck for the Tek-CreER^T2 mice, and Birgit Spiznagel for the CMV-Cre mice. We thank Konstantinos Gkatzis, Arica Beisaw, and Viola Graef for critically reading the manuscript. This work was supported by funds from the Max Planck Society to D.Y.R.S.

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Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, Bad Nauheim, 61231, Germany
Hyun-Taek Kim, Wenguang Yin, Paolo Panza, Felix Gunawan, Beate Grohmann, Carmen Buettner & Didier Y. R. Stainier
Department of Pharmacology, Max Planck Institute for Heart and Lung Research, Bad Nauheim, 61231, Germany
Young-June Jin & Stefan Offermanns
Scientific Service Group of Biomolecular Mass Spectrometry, Max Planck Institute for Heart and Lung Research, Bad Nauheim, 61231, Germany
Anna M. Sokol & Johannes Graumann
ECCPS Bioinformatics and Deep Sequencing Platform, Max Planck Institute for Heart and Lung Research, Bad Nauheim, 61231, Germany
Jens Preussner, Stefan Guenther & Mario Looso
Scientific Service Group of Morphometry, Max Planck Institute for Heart and Lung Research, Bad Nauheim, 61231, Germany
Sawa Kostin
Biobank, University of Giessen & Marburg Lung Center (UGLMC), Giessen, 35392, Germany
Clemens Ruppert & Andreas Guenther
Bioinformatics Core, Weill Cornell Medicine - Qatar, Doha, PO 24144, Qatar
Aditya M. Bhagwat
Laboratory of Molecular Cardiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20814, USA
Xuefei Ma & Robert S. Adelstein
German Centre for Cardiovascular Research (DZHK), Partner Site Rhine-Main, Frankfurt, 60323, Germany
Johannes Graumann, Stefan Offermanns & Didier Y. R. Stainier

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Hyun-Taek Kim
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Wenguang Yin
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Young-June Jin
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Contributions

H.-T.K. designed and performed experiments, and wrote the manuscript; W.Y., P.P., F.G., B.G., and C.B. contributed to data analysis and experiments; H.-T.K., Y.-J.J., and S.O. designed and performed in vitro studies; S.K. performed TEM analysis; A.M.S., A.M.B., J.G., and H.-T.K. performed mass spectrometry and data analysis; S.G. and H.-T.K. performed RNA-sequencing and data analysis; J.P. and M.L. performed data analysis for whole-exome sequencing; X.M. and R.S.A. provided Myh10^flox mice and Myh10 KO embryonic fibroblasts; C.R. and A.G. provided human patient samples; D.Y.R.S. conceived the ENU screen, enabled and supervised the project, analyzed data, and edited the manuscript. All authors commented on the manuscript.

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Correspondence to Hyun-Taek Kim or Didier Y. R. Stainier.

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Kim, HT., Yin, W., Jin, YJ. et al. Myh10 deficiency leads to defective extracellular matrix remodeling and pulmonary disease. Nat Commun 9, 4600 (2018). https://doi.org/10.1038/s41467-018-06833-7

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Received: 27 December 2017
Accepted: 25 September 2018
Published: 02 November 2018
DOI: https://doi.org/10.1038/s41467-018-06833-7

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