Fig. 4 | Nature Communications

Fig. 4

From: Epistasis studies reveal redundancy among calcium-dependent protein kinases in motility and invasion of malaria parasites

Fig. 4

The CDPK4 substrate SOC6 is important for the IMC stability in merozoite and ookinete. a Effect of soc6 deletion on the growth of asexual blood stage parasites (error bars show standard deviations from 2 independent infections). b Number of merozoites per schizont in control and SOC6-KO parasites (data shown is from 4 independent in vitro cultures). c Parasitaemia observed 1 h after the intravenous injection of control or SOC6-KO mature segmented schizonts (data shown is from four independent schizont cultures, two-tailed t-test). d Electron microscopy analysis of mature SOC6-KO and WT schizonts (error bars show standard deviations from duplicates, nWT = 140, nSOC6-KO = 267). e Ookinete conversion rate of 2.34 and SOC6-KO lines (error bars are standard deviations from three independent ookinete cultures, two-tailed t-test). f Ultrastructural analysis of WT and SOC6-KO highlighting the integrity of the IMC in WT and SOC6-KO p28-expressing cells (error bars are standard deviations from the mean, two ookinete cultures, nWT = 34, nSOC6-KO = 27). g Representative images of IMC defects observed in SOC6-KO parasites. Scale bar: 2.34, 1 µm (lower magnification) and 200 nm (higher magnification); SOC6-KO, 1 µm (lower magnification) and 500 nm (higher magnification)

Back to article page