Fig. 3 | Nature Communications

Fig. 3

From: Epistasis studies reveal redundancy among calcium-dependent protein kinases in motility and invasion of malaria parasites

Fig. 3

CDPK4 co-immunoprecipitates with components of the glideosome and the IMC. a Western blots and immunofluorescence analysis of schizonts expressing endogenously 3xHA tagged cdpk4, gap40, cdpk1 and soc6 alleles. Scale bars are 1 µm. b Protein interactions between IMC or glideosome proteins identified from GAP40-3xHA, SOC6-3xHA, MyoE-3xHA, CDPK4-3xHA and CDPK1-3xHA immunoprecipitates. Thick bars indicate that the interaction was identified from both immunoprecipitates. Blue and green filled circles denote the number of residues phosphorylated by CDPK4 ex vivo and by recombinant CDPK1 in vitro, respectively. The colour of the circle around the protein name indicates the requirement of its encoding gene for growth in asexual blood stages: red is essential, green is redundant; black denotes that the gene essentiality was not tested. c Effect of gap40 mutagenesis on the growth of asexual blood stage parasites (error bars show standard deviations from 2 independent infections). d Electron microscopy analysis of mature WT and GAP40S448/449A-3xHA schizonts (error bars show standard deviations from the mean; duplicates; two-tailed t-test; nWT = 140, nGAP40S448/449A-3xHA = 132)

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