Fig. 3 | Nature Communications

Fig. 3

From: Antigen-selective modulation of AAV immunogenicity with tolerogenic rapamycin nanoparticles enables successful vector re-administration

Fig. 3

Enhanced liver transduction via repeated AAV8 vector administrations in nonhuman primates. a Dual immunofluorescence staining of Gaa and hF.IX in livers from nonhuman primates treated with AAV8 vectors together with SVP[Rapa] (n = 2, SVP[Rapa]#1 and SVP[Rapa]#2, 3 mg kg1) or SVP[empty] control (n = 1). 95 days post AAV8-Gaa administration, liver lobes were collected and stained for Gaa (shown in green), hF.IX (shown in red) and Gaa/hF.IX co-localization (merge, shown in yellow) and nuclei are DAPI-stained (Blue). Scale bar 20 μm. b Quantification of cells positive for Gaa, hF.IX and double-positives to Gaa/hF.IX in liver lobes. Data are shown as mean ± s.d. (four different images per lobe were analyzed for each animal)