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Metallo-polyelectrolytes as a class of ionic macromolecules for functional materials


The fields of soft polymers and macromolecular sciences have enjoyed a unique combination of metals and organic frameworks in the name of metallopolymers or organometallic polymers. When metallopolymers carry charged groups, they form a class of metal-containing polyelectrolytes or metallo-polyelectrolytes. This review identifies the unique properties and functions of metallo-polyelectrolytes compared with conventional organo-polyelectrolytes, in the hope of shedding light on the formation of functional materials with intriguing applications and potential benefits. It concludes with a critical perspective on the challenges and hurdles for metallo-polyelectrolytes, especially experimental quantitative analysis and theoretical modeling of ionic binding.


Polyelectrolytes are a class of macromolecules containing charged groups, either positively, negatively or both1. Equally important to uncharged neutral polymers, polyelectrolytes have been widely used for a myriad of applications, ranging from genetic coding to synthetic membranes for separation to drug delivery, to name just a few2,3,4. Quaternary ammonium-based cationic and organic acid-based anionic polymers are among the most common polyelectrolytes. While polyelectrolytes continue to attract attention in the areas of chemistry, physics, engineering, biology, and pharmaceutics, it is appealing for both academia and industry to design charged macromolecules toward unprecedented properties and functions to address problems that the world is facing regarding health, energy, environments and sustainability in the 21st century5,6,7,8.

The fields of polymer and macromolecular sciences have enjoyed a unique combination of metals and soft organic frameworks in the name of metallopolymers, metal-containing polymers or organometallic polymers since the middle of 20th century9,10,11,12. When metallopolymers carry charged groups, they form a class of polyelectrolytes or metallo-polyelectrolytes. This is an emerging area that is particularly well suited for manufacturing functional materials. For convenience in this review, we refer traditional all-organic polyelectrolytes as to organo-polyelectrolytes.

Depending on the metal location and the nature of chemical bonding of metals with organic frameworks, metallo-polyelectrolytes have a variety of topologies as summarized in Fig. 1. In the case of metals as a pendant group, metal cations can be counterions to anionic polyelectrolytes (Fig. 1a). Polyelectrolyte frameworks could also carry neutral metal centers (Fig. 1b). In both cases, the charged groups in these polymers are still all organic, thus not being further discussed in this review unless mentioned specifically. On the other hand, metal cations can coordinate or covalently complex at the side chain to form metallo-polyelectrolytes (Fig. 1c, d). Similarly, main-chain metallo-polyelectrolytes can be also fabricated via coordination or covalent bonding (Fig. 1e, f). Organo-polyelectrolytes generally utilize covalent bonding to form ionic charged groups as an integral part of macromolecules. However, the formation of ionic centers via coordination chemistry is rare in this kind of polyelectrolytes.

Fig. 1
figure 1

Diverse topologies of metallo-polyelectrolytes. a metal ions attached to the side chain of organic polyelectrolytes through electrostatic interaction; b neutral metal complexes at the side chain of polyelectrolytes; c charged metal complexes at the side chain by covalent bonding; d charged metal complexes at the side chain through coordination; e main chain metallo-polyelectrolytes formed by coordination; f main chain metallo-polyelectrolytes formed by covalent bonding

Based on different architectures of metallo-polyelectrolytes, there are two major approaches to integrate metals into macromolecules: (1) metal ions attached to conventional polyelectrolytes through electrostatic interaction (Fig. 1a) or neutral metals (or metal complexes) added onto conventional polyelectrolytes (Fig. 1b), in which the charged groups behave most likely the same way as organo-polyelectrolytes, though the metals could provide additional functions; (2) charged metals integrated into organic frameworks (Fig. 1c–f), in which the properties of polyelectrolytes would be mostly dictated by the ionic metal centers. The latter scenario is expected to be substantially different from traditional organo-polyelectrolytes. As shown in Fig. 2, this short review articulates properties and functions of the second type of metallo-polyelectrolytes, in the hope of shedding light on how to harness their fundamental physiochemical properties for applications in biomedical sciences and advanced materials, as well as to present the most exciting discoveries for the broad communities of polyelectrolytes and macromolecules. Particularly, some of our recent research efforts are elicited to exemplify the benefits. While there are a few recent reviews on metal-containing polymers8,11,13,14,15, an examination on emerging directions of metallo-polyelectrolytes has not been done, to the best of our knowledge. We organize this review according to the following: (1) electronic, bonding, and redox properties; (2) functional materials via redox or electrostatic interactions; (3) ion-exchange for transport.

Fig. 2
figure 2

An overarching summary to illustrate a few key functions of metallo-polyelectrolytes. a most common transition metals for building metallo-polyelectrolytes; b a few properties of metallo-polyelectrolytes including electrostatic binding, redox and physiochemical stability; c applications in the areas of polyelectrolyte multilayers, antimicrobials, gene delivery and ion exchange for transport

Electronic, bonding, and redox properties

Polyelectrolytes are ionic macromolecules that can complex with a variety of oppositely charged substrates. The ionic groups and polymeric backbone are the most important components for polyelectrolytes. When mixing with substrates carrying oppositely charged ions, it typically encounters enthalpy-driven electrostatic interactions. The association between oppositely charged molecules simultaneously initiates entropic changes by the loss of counterions. Most often, the entropic change drives the complexation, while the attractive Coulombic interactions between opposite charges are favorable in terms of enthalpy change. On the other hand, complexes between two oppositely charged polyelectrolytes, often referred to as polyelectrolyte complexes (PECs), are mostly studied16,17. Depending on the strength of complexation, PECs often undergo phase separation, with the formation of either solid precipitates or liquid-like coacervates18,19.

While there are many similarities to organo-polyelectrolytes, metallo-polyelectrolytes provide a different level of electrolyte chemistry and physics. Noticeable difference is the presence of transition metals that allow unique electronic properties such as lipophobicity, ionic binding strength and redox chemistry20,21. The tunable oxidation state of metal cations would offer unprecedented redox chemistry that organo-ions typically cannot. This property could open up many new applications for metallo-polyelectrolytes beyond traditional organo-polyelectrolytes8,22. In addition, metal cations may exhibit significantly distinct behaviors in thermal and chemical stability for some applications23,24. On the other hand, metals are generally used as cationic centers for building polyelectrolytes, thus it is fair to compare metallo-polyelectrolytes with cationic organo-polyelectrolytes, particularly the most studied quaternary ammonium-based counterparts.

Electronic properties of metallo-polyelectrolytes

The ionic binding for both metal-containing cations and organo-ions first encounters with electrostatic Coulombic interactions. The intrinsic electron configuration of transition metals often involves incompletely filled d-orbitals, which could have dramatically different electronic and possibly solvophilic effects in comparison with nitrogen and phosphorous atoms in organo-ions, when complexing with oppositely charged molecules. In the case of organometallic cations, the size of these cations matters. The Pearson theory of hard and soft acids and bases (HSAB theory) could provide a basic foundation to compare the ionic binding strength25,26,27. Strong binding occurs between hard acids and hard bases, similarly between soft acids and soft bases. Small quaternary ammonium is a hard acid and prefers to interact with small anions. In comparison, most metallo-polyelectrolytes carry large metal cations (or large organometallic cations), a kind of soft acids. Thus, they would favor to bind with soft bases, large anionic substrates20. Although there is lack of quantitative data on ionic binding strength in literature, the qualitative difference between organic and metal-containing ions should be considered for choosing counterions, whenever appropriate.

Ionic binding of metallo-polyelectrolytes

We examine interactions of metallo-polyelectrolytes with several oppositely charged substrates, which provide a general picture to illustrate various scenarios. Specific comparisons with organo-polyelectrolytes are given in following sections outlining functional materials. There are three levels of oppositely charged molecular substrates for interactions relevant to the functions of metallo-polyelectrolytes: small molecules, macromolecules, and membranes or crosslinked networks (Fig. 3). All these interactions are associated with changes of enthalpy, entropy, and in many cases dehydration (e.g. reduced solubility with loss of water).

Fig. 3
figure 3

Metallo-polyelectrolyte interactions with oppositely charged molecular substrates at three different levels. a small molecules, b macromolecules, and c membranes or crosslinked networks

In the case of molecular anions (Case a), the entropic change with the release of counterions associated with metal cations is generally small28. Thus, the enthalpic change would play a greater role in driving ion exchange. It has been demonstrated that the type of charges appears to be more important29. For example, carboxylates associate with cations weaker than sulfonates. The aromatic sulfonates associate more strongly than the aliphatic ones. The presence of π-system on anions increases the affinity for cationic metal centers significantly. This is mostly attributed to Coulombic interactions. Another driving force would be the reduction of solubility in solutions, in some case even leading to phase separation. The complexation of metallo-polyelectrolytes with molecular anions is essentially an equilibrium process, which could be dramatically shifted with the significant reduction of solubility or precipitation of newly formed complexes. For example, a phase-transfer ion-exchange strategy was developed to prepare cationic cobaltocenium polyelectrolytes using tetrabutyl ammonium halide salts to replace a hexafluorophosphate counterion30. The dramatically reduced solubility crashes out newly formed halide-paired polyelectrolytes in acetonitrile. On the other hand, halide-paired polyelectrolytes were reported to ion-exchange with antibiotics to form bioconjugates31. The exchange is favorable, possibly due to the small gain of entropy, as antibiotics have a significantly larger size than halides. In addition, one should consider the Pearson HSAB theory on soft acids and bases25,26,27. In the case of ion-exchange from chloride to β-lactam antibiotics, the anionic antibiotics are a relatively softer base than chloride, thus favorable for interaction with a soft acid like cobaltocenium.

When metallo-polyelectrolytes interact with negatively charged macromolecules, it is the situation of Case b for the formation of PECs. While the complexation starts with attractive electrostatic interactions, it is the favorable entropic change to drive the mixing by releasing their respective small counterions. The loss of configurational and translational entropy of the long chains of polyelectrolytes upon the formation of PECs would contribute positively to the entropy change. The PEC formation is dictated by many factors such as molecular weight, charge density, molar ratio, Van der Waals forces, hydrophobic interactions, hydrogen bonding, etc. Typically, polymers with a longer chain and higher charge densities tend to form PECs. It has been quantitatively compared with ionic binding strength between oppositely charged polyelectrolytes29,32. We reported the complexation of cobaltocenium-containing methacrylate polymers with poly(acrylic acid). The level of complexation was monitored by the release of their counterions. The gradual increase of turbidity indicated the formation of liquid-like coacervates31. However, when these metallo-polyelectrolytes interact with phosphate-containing macromolecules (e.g. lipoteichoic acid), it was observed with the formation of precipitates. In addition, when complexed with the same anionic polyelectrolytes (e.g. poly(styrene sulfonate)), cobaltocenium-containing polyelectrolytes exhibited stronger binding strength than quaternary ammonium-containing counterparts20.

The ionic binding strength can be evaluated by the extent to which ion pairs between PECs are torn apart by a common salt, KBr29,33. With the addition of salt, ion pairs in PECs are surrounded by the free ions via diffusion. With the increase of salt concentration, the PECs dissociate progressively. The complexation would be completely suppressed above a critical ionic strength, which depends on the type of ionic groups and charge density in polyelectrolytes as well as their chain length. Thus, ionic strength is always an important factor regarding polyelectrolyte complexation.

As a membrane or a crosslinked charged network possesses even lower translational and rotational configurations than a single polyelectrolyte (Case c), it is expected that the positive gain of entropy change would greatly favor its complexation with metallo-polyelectrolytes via releasing highly mobile counterions. On the other hand, the strong complexation might have a negative impact on the diffusion of polyelectrolytes across the network. The choice of ionic groups and charge density would become more important for tuning the complexation. It has been demonstrated that diffusion of ionic sites within PEC films is much faster than polyelectrolytes themselves (probably over 2 orders of magnitude)34. This is quite straightforward as it is a local rearrangement of ionic units vs. PEC assembly in all of their forms. Such diffusion becomes more relevant when the networks are cell membranes, which will be elaborated in a section below about antimicrobial biomaterials.

Redox chemistry of metallo-polyelectrolytes

Research on redox active polyelectrolytes was actively explored in early 1980s35,36,37,38,39,40,41,42. For metallo-polyelectrolytes, metal-containing cations can be well utilized with their reduction and oxidation chemistry43, which is usually not possessed by organo-ions. For example, many research groups have extensively investigated the redox behaviors of ferrocenium and cobaltocenium-containing polyelectrolytes, including dendrimers and block copolymers44,45,46,47,48,49,50,51. The formation of PECs can be tuned by changing the oxidation state of metal-containing cations, in some cases, even fully destroyed. In the section below regarding multilayers, redox chemistry of metallo-polyelectrolytes could be utilized to modulate the assembly and disassembly processes. In biological systems, metal cations could participate metabolisms involving enzymes, such as oxidative stress52,53. Fenton chemistry is another common redox chemistry producing super radical species54, which has been reported in designing antimicrobial metallo-polyelectrolytes to induce oxidative stress on bacterial cells55,56, as elaborated below.

Functional materials via redox or electrostatic interactions

Polyelectrolyte multilayers (PEMs)

The formation of PECs is mostly exemplified by polyelectrolyte multilayers (PEMs), in which alternate polyanions and polycations are stacked on each other57,58. It is mostly an entropy-driven process. The loss of translational and configurational entropy is out-competed with entropic gains by releasing small counterions. Given diverse applications from functional coatings to advanced drug delivery vehicles, PEMs are typically constructed via strategies such as layer-by-layer, surface grafting, and Langmuir-Blodgett, which provide facile control over the surface characteristics and functionalities59,60. Different from organo-polyelectrolytes, the metal centers endow metallo-polyelectrolytes with tunable redox, photoluminescent, magnetic, and catalytic properties, and open up new horizons towards multifunctional smart PEMs61,62,63,64.

One of the key challenges in conventional PEMs lies in the controlled disassembly. Taking advantage of the redox-induced change of charged states of metal centers, many groups reported electrically controlled disassembly of PEMs based on metallo-polyelectrolytes65,66,67. It was found that PEMs constructed from a positively charged cobaltocenium-containing polyelectrolyte (PMCl) and negatively charged poly(styrene sulfonate) (PSS) underwent controlled disassembly upon electrical reduction of cobaltocenium65. This process is almost linearly dependent on the electrical reduction time, resulting in complete disintegration of PEMs in several minutes. This kind of electroactive PEMs was further explored as a trigger to control the release of non-responsive molecules in an overlaying layer. As shown in Fig. 4a, PEMs were assembled with the following compositions and sequence: PMCl(PSS/PMCl)(PSS/PDDA)3. Reduction of cobaltocenium caused a rapid and complete loss of the layers on top of PMCl(PSS/PMCl). Owing to the mild and controlled reduction of cobaltocenium, such kind of PEMs can be potentially used as effective triggers for redox-controlled release of analytes that are not responsive and may be sensitive to harsh redox conditions, such as drugs and antibodies.

Fig. 4
figure 4

Controlled disassembly and controlled swelling of metallo-polyelectrolyte based PEMs. a reduction-induced disassembly of cobaltocenium-based PEMs65, adapted from ref.65 with permission from The Royal Society of Chemistry; b a strategy used in controlled swelling of PEMs72, adapted from ref.72 with permission, Copyright©2014, Elsevier

How to control mechanical properties and swellability of PEMs is critical for many applications such as tissue engineering and drug delivery21,68. In theory, any influxes that contribute to the osmotic pressure and the electrostatic repulsion of likely charged polyelectrolytes may cause PEMs to swell. In the case of metallo-polyelectrolytes, once the redox state of metal centers is changed, corresponding counterions have to diffuse out of the multilayers to compensate for the missing charges. As a result of the increase in osmotic pressure, the PEMs start to swell due to their elastic polymer deformations69. Different redox-active metal centers involving ferrocene, Os(bpy)2PyCl+, or Os(CN)6Py3−/2− have been used in electrochemical swelling of PEMs21,70,71. Calvo et al. reported the first example of electrochemical swelling of PEMs70, as shown in Fig. 4b72. They used osmium complex–containing PAH (poly(allylaminehydrochloride)) and glucoseoxidase (GOx) or PSS to assemble PEM films. It was found that during the oxidation/reduction of osmium complexes, the PEMs underwent reversible swelling and deswelling. It was attributed to the incorporation or exclusion of counterions and/or water molecules from the electrolytic solution70.

The presence of metal centers in metallo-polyelectrolytes could endow PEMs with catalytic properties. There are two major approaches to fabricating this kind of PEMs. One approach is to install catalytic sites in PEMs by using precursor-containing polyelectrolytes. Rahim et al. used a polyelectrolyte complex of silver ion-doped polyethyleneimine (PEI) and poly(acrylic acid) (PAA) to fabricate PEMs73. After in-situ reduction of Ag+ to Ag nanoparticle, it was found that the hybrid films exhibited an enhanced catalytic activity on the reduction of 4-nitrophenol. The other method uses charged coordination supramolecular polyelectrolytes. For example, Liang et al. prepared PEMs with dual electrocatalytic activity by using supramolecular metallo-polyelectrolytes carrying Fe3+ and a bis-ligand74. These films may lower the half-wave potential for different kinds of electroactive molecules and enhance the current for neutral or oppositely charged electroactive species, which are attributed to the mediation of metallo-polyelectrolytes in the electron transfer process and the accumulation of electrons in the films. The thickness of PEMs is very crucial in this case, which may affect the depth where the exchange of electrons occurs. If the thickness is less than 25 nm, there are some uncovered sites on the electrode, therefore, the electroactive species can diffuse quickly to the exposed electrode surface before electron exchange occurs. While in the case of a thicker film, the electrode is covered completely, which prevents the diffusion of electroactive species. Miao and coworkers prepared side-chain metal cluster-based polymers through ring-opening metathesis polymerization75. The metal centers in the polyoxometalate cluster are at their highest charged state, which makes the cluster a potential catalyst for oxidation. By using a model oxidation reaction of tetrahydrothiophene, they compared the catalytic property of both monomer and polyelectrolyte. Quantitative experiments demonstrated that the polymer showed a better catalytic activity than its corresponding monomer.

PEMs from metallo-polyelectrolytes, especially metallo-supramolecular coordination polyelectrolytes (MEPEs)76,77, demonstrate reversible electrochromism with applications including dynamic windows, panel displays and smart paper. Most MEPEs are constructed by ditopic ligands, such as bis-terpyridine and its analogues, and transition metal ions (Fe2+, Co2+, Ni2+, Zn2+, Ru2+, etc). Typically, the electrochromic properties of this system are related to the redox-dependent metal-to-ligand charge transfer (MLCT)78, as well as the nature of substituents and spacers79. For example, the Kurth group reported a series of MEPEs with bis-terpyridines with different conjugated spacers80. They found that the length of spacers has an influence on the color of PEM films. Upon the increase of electrical potential from 0 to +1.6 V (vs. Ag/AgCl), the absorbance of MLCT at ca. 585 nm decreased, and the color of films decayed from dark-blue to colorless. Full recovery of MLCT can be achieved by decreasing the potential back to 0 V. The electrochromism was almost fully reversible over 150 cycles. In another case, by using cationic viologen group as a spacer, Zhang and coworkers prepared electrochromic PEMs using a Langmuir-Blodgett method for alternate deposition of MEPE and PSS79. Upon the reduction of ligand with an applied potential of −1.1 V vs. Ag/AgCl, films changed from colorless to light red, which was also reversible after removal of the applied potential (−1.1 V).

Compared with organo-polyelectrolytes, magnetic properties are another unique characteristic for metallo-polyelectrolytes. By carefully selecting metal ions and ligands, magnetic metallo-polyelectrolytes could have tunable magnetic properties81,82,83,84,85. Owing to the decrease of the crystal field splitting of d-orbital subsets, metal ions in metallo-polyelectrolytes may undergo a spin transition from a low-spin to a high-spin state, resulting in changes of magnetic properties. By combining ditopic ligand and Fe2+, the Kurth group reported a metallo-supramolecular coordination polyelectrolyte wrapped with flexible alkyl chains, which are electrostatically encapsulated with flexible quaternized surfactants82. Subsequently followed by a Langmuir–Blodgett technique, multilayers were prepared. The hexagonally packed alkyl chains became disordered upon heating, resulting in a distortion of the metal ion coordination geometry. The changes in the coordination geometry of the metal complex resulted in a reversible spin crossover (SCO) between a diamagnetic low-spin state and a paramagnetic high-spin state. Besides heat-induced SCO, Yan and coworkers also reported a redox-induced SCO system based on a polyelectrolyte complex between a cationic organo-polyelectrolyte and a coordination metallo-polyelectrolyte from ditopic ligand and Fe2+83. Upon oxidation to Fe3+, the polyelectrolyte complex became paramagnetic due to the presence of an odd number of unpaired electrons, which resulted in a decrease of both the spin–lattice relaxation time T1 and the spin–spin relaxation time T2 of protons in water.

Taking the advantage of UV absorption ability of metallo-polyelectrolytes and its conversion to heat, Burnworth and coworkers reported an optically healable supramolecular metallo-polyelectrolyte86. On exposure to ultraviolet light, the metal complexes can be electronically excited, and the absorbed energy is converted into heat. This causes temporary disengagement of the metal-ligand motifs and subsequent reversible decrease in the molecular weight and viscosity of the polyelectrolyte, thereby allowing quick and efficient healing of defects. The photoluminescent properties of metallo-polyelectrolytes can be also used in label-free detection of proteins. For example, a cationic platinum-terpyridine complex and anionic poly(phenylene ethynylene) with sulfonate groups in the side chain (PPE-SO3) were used to form a polyelectrolyte complex87,88. They found Fӧrster resonance energy transfer (FRET) from the polymer backbone to the metal complex can be tuned upon interactions with biomacromolecules, which can be used to detect human serum albumin.

Besides PEMs, another method to construct surfaces with metallo-polyelectrolytes is self-assembled monolayers (SAMs)89, which allow precise control of location and morphology. Traditional SAMs are usually fabricated from functional thiols and silanes. While in the case of metallo-polyelectrolytes, the Geiger group developed a covalent modification method through cathodic reduction of cyclopentadienyldiazonium complexes90. Cobaltocenium with diazonium groups underwent facile cyclopentadienyldiazonium ligand-based one-electron reduction on the surface of a glassy carbon electrode. Accompanied by the release of nitrogen gas, a metal-containing monolayer was installed on the electrode. Due to the nature of covalent bonding, these monolayers are quite robust with long-time storage. Recently, they also achieved anodic oxidation of cobaltocenium monolayer-modified electrodes91. By incorporating metallo-polyelectrolytes or metal precursors, the swelling of SAMs can be tuned as a function of electrode potentials92.

Antimicrobial biomaterials: ionic interactions with cells

Via enthalpy or entropy or both driven ion exchange, metallo-polyelectrolytes can bind to small anionic molecules like antibiotics, negatively charged macromolecules like peptides, enzymes and proteins32,93, as well as anionic networks like cell walls and membranes94. Taken together with the redox metal centers, metallo-polyelectrolytes can facilitate intracellular biochemical processes, thus providing unprecedented opportunities for antimicrobial applications.

Drug resistance has become an increasingly intractable issue that leads humanity towards a post-antibiotic era95. The discovery of next-generation antimicrobial agents is aimed to address this mounting crisis. As a comparison to small molecular antibiotics, antimicrobial macromolecules are usually more chemically robust, less volatile, with longer lifetime of killing efficacy and superior environmental compatibility56,96,97,98,99. Among them, metallo-polyelectrolytes show some of unique characteristic as they integrate multiple antimicrobial moieties into a macromolecular scaffold. Currently, various types of metals, including iron, cobalt, tin, copper, nickel, ruthenium, platinum, gold, silver, zirconium, titanium, vanadium, chromium, cadmium, manganese, zinc, germanium gallium and lanthanum, have been integrated into macromolecular architectures to achieve antimicrobial properties8,56,100,101. Among them, iron, cobalt, ruthenium, copper and silver are mostly explored. There are two major types of antimicrobial mechanisms of action by metallo-polyelectrolytes: (1) metal ions provide the antimicrobial activity; (2) metallo-polyelectrolyte bioconjugates with antibiotics enhance antimicrobial activity.

In the first case, metal cations can directly chelate with various intracellular biomacromolecules like enzymes and proteins to modulate biochemical processes that mediate antimicrobial behaviors. Metal cations have been incorporated into main chain or side chain macromolecular frameworks, which can be transported to specific cellular environments. For example, ruthenium ion can form a complex by coordinating with pyridine through a flexible polymethylene linker to yield ruthenium-based main-chain polyelectrolytes (Fig. 5a). The Keene group reported multi-nuclear polypyridyl ruthenium(II) complexes as antimicrobial agents that can accumulate on bacterial cells. The ruthenium complexes interfere with key cellular processes to kill bacteria102,103,104. Hassan et al. reported a side-chain water-soluble cupric cellulose hybrid via a supramolecular strategy with tetrafluoroborate as the counter anion (Fig. 5b)105. This copper-based polyelectrolyte greatly inhibited the growth of Gram-negative E. coli and Gram-positive S. thermophiles and S. aureus by blocking metal-binding sites in enzymes and disturbing the cell respiration process. In addition to specific intracellular biomedical processes involving metal-ligand binding, the tunable oxidation state of metal cations would offer unique redox chemistry that enables Fenton-type reaction to trigger cellular oxidative stress on bacteria55,106. Iron-based polyelectrolytes utilize a cationic derivative of ferrocene, termed η6-arene-η5-cyclopentadienyl iron(II) complex (Fig. 5c), reported by Abd-El-Aziz and coworkers107,108,109,110. The iron complex-containing linear polymers and dendrimers can induce cellular oxidative stress on bacteria due to the redox-active cationic iron center. A simple illustration of Fenton chemistry of ferrocene is given in Fig. 5e111,112. In addition, the antimicrobial activity can be easily tuned by changing the counter anion108.

Fig. 5
figure 5

Structural units of antimicrobial metallo-polyelectrolytes. a polypyridylruthenium(II) complex polymer;102,103,104 b copper-terpyridine carboxymethyl cellulose polymer;105 c η6-arene-η5-cyclopentadienyliron(II) complex polymer;107,108,109,110 d cobaltocenium polymer;117 e Fenton chemistry involving redox of ferrocene111

Given the cationic nature of metallo-polyelectrolytes, they could ion-pair with anionic peptides to form bioconjugates, which have shown some of unique antimicrobial efficacy, especially towards drug-resistant bacteria, in comparison to unconjugated polyelectrolytes. Joyner et al.113 reported that metallo-peptides containing cupric ion complexes showed more enhanced antimicrobial activities than each structural unit alone. They claimed that metal ion-containing peptides irreversibly cleaved cellular nuclei acids. In addition, the charged metal complexes can promote oxidation with proteins, redox cofactors, or initiate a cellular oxidative stress response that ultimately damaged bacterial cells. In addition to cupric ions, cationic cobaltocenium ions can also bind with peptides. Metzler-Nolte and coworkers reported a series of cobaltocenium-containing polypeptides. The antimicrobial activities towards Gram-positive S. aureus and Gram-negative E. coli and P. aeruginosa were enhanced due to the increased cationic charge units from cobaltocenium, which strengthened the electrostatic binding to negatively charged bacterial membranes114,115,116.

In addition to peptides, the affinity of cationic cobaltocenium species with anionic substrates enables them to form bioconjugates with many traditional antibiotics via electrostatic binding. Recently, the Tang group reported cobaltocenium-containing polymers as metallo-polyelectrolytes for antimicrobials31,117,118, as shown in Fig. 5d. These metallo-polyelectrolytes can conjugate with widely used β-lactam antibiotics such as penicillin-G, amoxicillin and ampicillin, providing a unique macromolecular scaffold to revitalize traditional antibiotics to kill multidrug-resistant bacteria. As indicated in Fig. 6, antibiotic-loading quantities could be mediated by the cobaltocenium moiety on a polyelectrolyte chain. When cobaltocenium polyelectrolytes come closer to the crosslinked cell wall, the electrostatic interactions with negative lipoteichoic acid facilitate binding of metallo-polyelectrolytes with microbial cells. Alternatively, cationic cobaltocenium polyelectrolytes can strongly interact with anionic phospholipid on microbial cell membranes. These processes are possibly driven by the increase of entropy via releasing small antibiotics. The amphiphilic nature of polyelectrolytes would also promote insertion into membranes, leading to their disruption119.

Fig. 6
figure 6

Proposed interactions between metallo-polyelectrolytes and bacterial cells. It involves the following steps: electrostatic interactions between cationic metallo-polyelectrolytes and anionic membranes; surface binding on membranes, membrane insertion and eventual membrane disruption. Metallo-polyelectrolytes could also interact with lipoteichoic acid in the outer leaflet of Gram-positive bacteria

At the same time, cobaltocenium polyelectrolytes also act as β-lactamase inhibitors. β-Lactamase is an enzyme, which contains negative carboxyl groups in a few amino acid residuals. The PEC-like binding between metallo-polyelectrolytes and β-lactamase is an entropy-favorable process by releasing small counterions, effectively nullifying the activity of β-lactamase29. As observed by Lindhoud et al.120, a similar inhibition behavior of enzymatic activity occurred when they added more positively charged units onto polyelectrolytes. Therefore, cobaltocenium polyelectrolytes have shown synergistic effects to lyse drug-resistant bacterial cells whereas being nontoxic towards mammalian cells due to much weaker hydrophobic-hydrophobic interactions31,117. In addition, attempts were made to increase the antimicrobial activity in a more refined manner via structural modification. For example, boronic acid was introduced into a metallo-polyelectrolyte framework to make it adhering to lipopolysaccharide or peptidoglycan to further enhance interactions with cells117. Cobaltocenium-containing polyelectrolytes were also grafted from inorganic nanoparticles such as silica and iron oxide to increase the local drug concentration due to their high specific surface area121,122. Owing to the facile synthetic methodology and diverse well-established antibiotic counterparts, metallo-polyelectrolytes establish a platform of therapeutics against bacteria.

Organo-polyelectrolytes contain quaternary ammonium, phosphonium or sulfonium groups as the cationic centers in comparison to metallo-polyelectrolytes. The cationic groups can selectively bind to bacterial membranes via electrostatic interactions, subsequently hydrophobic components penetrate into the lipid layer, resulting in cell membrane disruption and eventually cell death. Metallo-polyelectrolytes could perform similar mechanisms of action, but much more beyond organo-polyelectrolytes. Metal cations can directly chelate with various intracellular biomacromolecules like enzymes or proteins whereas traditional organo-polyelectrolyte may not. When organo-polyelectrolytes are too hydrophilic, they are inactive in disrupting bacterial membranes. However, when the hydrophobicity is too high, they could be toxic towards mammalian cells, as driven by hydrophobic interactions with zwitterionic mammalian cell membranes. Therefore, the antimicrobial activity is achieved by the optimal compositions of organo-polyelectrolytes, sometimes the counterbalance between binding selectivity and membrane disruption can be a dilemma. In the case of the formation of bioconjugates, metallo-polyelectrolytes can circumvent such problem because only clinically approved antibiotics or peptides play a role in killing bacteria, while the metallo-polyelectrolyte framework facilitates their revitalization of efficacy by nullifying bacterial resistance pathways.

Gene delivery of polyplexes

As mentioned above, metallo-polyelectrolytes can form PECs with oppositely charged polyelectrolytes. When they interact with negatively charged biomacromolecules like protein, DNA and enzymes, they can form a complexation favorably by releasing small counterions. The state of PECs could be either liquid-like coacervates or solid precipitates, depending on the strength of complexation. The binding ability of cationic metallo-polyelectrolytes with anionic biomacromolecules could provide opportunities for gene delivery.

Nucleic acid-based therapeutics have gained extensive attention over the past two decades due to their promise for the treatment of many life-threatening diseases, ranging from genetic disorders to cancers123,124. Successful delivery of nucleic acids depends on the design of safe and efficient vectors3. There are two major delivery vectors to transport nucleic acid-based therapeutics including plasmid DNA and small interfering RNA (SiRNA): viral and non-viral vectors125. Non-viral vectors, especially synthetic cationic macromolecules, have precisely customized chemical structures and functionalities, leading to a great improvement in cost and safety compared to viral vectors126.

Via ionic interactions, a cationic polyelectrolyte can bind with DNA to form a polyplex, a terminology essentially same as PEC. The mostly explored polyelectrolytes are poly(L-lysine) and polyethylenimine as well as their modified variants125. Despite extensive research on these traditional organic amino-polyelectrolytes, examples of other polyelectrolytes show their burgeoning opportunities as alternatives127,128. To the best of our knowledge, the only reported metallo-polyelectrolytes with charged metal centers can be potentially used for gene delivery are cobaltocenium bioconjugates, as reported by Metzler-Nolte and coworkers115. They found that cobaltocenium-peptide bioconjugates exhibited active endocytosis and directed nuclear delivery, which are two key steps for gene release. Manners et al.129,130 prepared a main-chain cobaltocenium-containing water-soluble polyelectrolyte termed poly(cobaltoceniumethylene). Cationic cobaltocenium ions can induce conformational replications of an anionic DNA template through electrostatic interaction, demonstrating the efficient formation of polyplexes128,131. These efforts could inspire the use of cationic metallopolymers as gene delivery vehicles, as the formation of polyplexes would be the first step to carry genetic macromolecules.

A possible delivery of cobaltocenium polyelectrolyte-DNA polyplexes is proposed in Fig. 7. Polyelectrolytes with positively charged cobaltocenium can strongly bind with the anionic phosphodiester group of a nucleic acid to form colloidally stable polyplexes. Polyplexes enter cells via endocytosis and are trapped in endosomes. Then, rupture of endosomes and release of DNA to cytosol may occur through a proton sponge effect (pH-buffering)125. After an active endocytosis by cells, it forms an endo/lysosome. Then the acidic endo/lysosome microenvironment can hydrolyze the ester linkers in cobaltocenium copolymers, the membrane is disrupted by the hydrophobic chain segments, facilitating the release of DNA. Eventually, DNA chains penetrate into nucleus for gene therapy132.

Fig. 7
figure 7

Proposed dene delivery of non-viral metallo-polyelectrolytes. The delivery requires the formation of polyplexes between metallo-polyelectrolytes and nucleic acids. It involves endocytosis, endo/lysosome escape and release of nucleic acids

As a comparison to metallo-polyelectrolytes with charged metal centers, a polyelectrolyte with neutral metal centers used in gene delivery contains ferrocene as the organometallic moiety127. The charged centers are quaternary ammonium, which binds with the anionic DNA chain. The ferrocene moiety along the polyelectrolyte backbone can play a hydrophobic role or redox-active activity rather than electrostatic interaction133,134.

Ion-exchange for transport

Ion-exchange in polyelectrolyte-based membranes has a broad range of applications in energy conversion/storage devices, such as alkaline fuel cells135,136, redox flow batteries137, electrodialysis and electrolyzers138. It mainly takes the advantage of the ability of binding to small ions (like hydroxide, halide, carbonate ions) and redox stability under operational potentials. However, it remains a challenge to develop advanced anion-exchange membranes (AEMs) with sufficient chemical stability and high anionic conductivity under extremely harsh, highly basic conditions in alkaline fuel cells. The major issues that limit the performance of AEMs include the degradation of polymers under caustic conditions and lower intrinsic mobility of hydroxide ions. This section summarizes recent progress on the design of metallo-polyelectrolytes to improve ion exchange as well as cation stability under alkaline conditions.

Traditional quaternary ammonium-based organo-polyelectrolytes are used for anion-exchange and transport139,140,141. Some other organic cations include imidazolium142,143, phosphonium144,145, pyridinium146, and guanidinium147,148, while poor chemical and thermal stability is the major concern for many of these cations and their organo-polyelectrolytes. The primary mechanisms of degradation for the well-studied benzyl trimethylammonium cation involve Hofmann elimination and nucleophilic substitution by hydroxide ions, as well as the formation of Ylide or chemical rearrangements136,149.

Compared to conventional organic cations, many metal complexes (or metal cations) exhibit good physiochemical stability under strong basic conditions. For example, cationic Ru complexes exhibited excellent stability in NaOH solution at room temperature over six months23. Similarly, no significant changes were observed for cobaltocenium cyclooctene monomer in 1 M NaOH solution at 80 °C for two weeks24. Only 7% of dimethyl cobaltocenium cation degraded after storing in 1 M KOH at 80 °C for 30 days150. Permethyl cobaltocenium cation showed a much longer lifetime than many other organic cations under high temperature (only 8.5% degraded at 140 °C in 1 M NaOD/D2O after 6 weeks)151. In addition, both mono-substituted and permethyl cobaltocenium-containing polyelectrolytes showed excellent thermal stability with decomposition temperature over 300 °C, which is significantly higher than most ammonium or imidazole-functionalized polyelectrolytes24,151. However, more rigorous tests are required to reveal the stability of these metal complexes under highly basic and also highly oxidative conditions, especially at the device level152. In addition to metal-containing ions, polymeric frameworks are equally important to maintain structural and mechanical integrity of the entire polyelectrolytes.

On the other hand, to obtain higher ion conductivity, elucidation of the ion diffusion mechanisms inside polyelectrolyte membranes is vital34,153. In the case of alkaline fuel cells, free hydroxide ions are produced by oxygen reduction reaction in the cathode. Then, hydroxide ions can hop from one cationic site to another, as seen in Fig. 8f, which is similar to proton transport through the ionic domains in Nafion. Finally, free hydroxide ions transport through membranes to the anode side and participate the oxidation reaction with fuels. We reported cobaltocenium-containing copolymers with a hydrophobic polyethylene backbone for maintaining mechanical stability and hydrophilic cobaltocenium side chains for achieving rapid hydroxide transport24. To date, metallo-polyelectrolytes for ion-exchange membranes can be separated into two categories: polymers with non-aromatic based backbones and aromatic backbones.

Fig. 8
figure 8

Metallo-polyelectrolytes for ion transport. Synthesis of metallo-polyelectrolytes by ROMP with metal complexes at the side-chain: a heteroleptic bis(terpyridine) Ru(II) complex;23 b nickel-containing complex;154 c cobaltocenium cation24. Metallo-polyelectrolytes by polycondensation: d permethyl cobaltocenium-containing polysulfone;151 e cobaltocenium-containing polybenzimidazole;150 f illustration of single ion site migration inside alkaline anion-exchange membranes; g A highly preferred phase separated morphology containing continuous hydrophilic ion transport channels and a hydrophobic matrix155, adapted with permission from ref.155. Copyright © 2017, American Chemical Society

Non-aromatic based polymer backbones

This class of metallo-polyelectrolytes is mostly built from norbornene/cyclooctene-functionalized monomers, as shown in Fig. 8a–c. Hickner, Tew and co-workers reported multivalent metal-cation-based polyelectrolytes in 201223, which were synthesized by copolymerization of a bis(terpyridine)Ru(II) complex-functionalized norbornene monomer with dicyclopentadiene, the latter monomer is a crosslinker (Fig. 8a). These membranes exhibited good hydroxide conductivity (28.6 mS/cm at 30 °C) with mechanical properties comparable to traditional quaternary ammonium-based polyelectrolytes. In early 2018, they further developed crosslinked nickel-containing polyelectrolytes using thiol-ene reactions (Fig. 8b)154. In this case, the nickel cation functions as both an ion conductor and a crosslinker. Although the formation of ion clusters was observed, high water uptake ( > 150%) and extreme brittleness of membranes were the major drawbacks. We reported ring-opening metathesis polymerization (ROMP) of cobaltocenium-containing cyclooctene, followed by backbone hydrogenation, leading to a class of membranes with a polyethylene-like framework and alkaline-stable cobaltocenium cation (Fig. 8c)24. These polyelectrolyte membranes showed high hydroxide conductivity (90 mS/cm at 90 °C) and excellent durability over one month.

Aromatic based polymer backbones

The second type of metallo-polyelectrolytes contains aromatic polymer backbones. These polymers are typically prepared by polycondensation reactions. The Yan group reported permethyl cobaltocenium as an ultra-stable cation for ion transport151. The bulky hydrophilic cations were linked to a rigid hydrophobic polysulfone backbone via a flexible diamine linker (Fig. 8d). The membrane exhibited excellent thermal stability, high tensile strength (40 MPa with 10% elongation at break) and good conductivity (22 mS/cm at room temperature). However, earlier work demonstrated that polysulfone-based membranes would have cation-triggered backbone degradation149, which remains a concern for durability of these materials. Polybenzimidazole membranes with cobaltocenium on the backbone were prepared by microwave polycondensation, as reported by Zhu and co-workers (Fig. 8e)150. These membranes exhibited hydroxide conductivity of 37.5 mS/cm at 90 °C and over 80% hydroxide conductivity retention for nearly one month.

Ideally, a phase-separated morphology in membranes is favorable to facilitate the ion transport and improve the electrochemical performance, especially under partially hydrated conditions, as illustrated in Fig. 8g155. Well-connected hydrophilic domains or nanochannels for faster ion migration were also observed in many polymer architectures like block copolymers and comb-shaped polymers156. On the other hand, hydrophobic components could serve as the matrix to maintain structural and mechanical integrity during ion transport and device fabrication.

Conclusions and perspectives

The field of metallo-polyelectrolytes is just beginning to take off, as a result of their fascinating electronic, physicochemical and redox properties that are recently discovered as quite differently from organo-polyelectrolyte counterparts. The unique electronic properties of metal centers endow metallo-polyelectrolytes some of unprecedented ionic binding with a variety of (macro)molecular substrates. They could complex with small antibiotics, oppositely charged polyelectrolytes such as DNA and peptides, as well as cell membranes or crosslinked networks. The redox properties further allow metallo-polyelectrolytes to access many functions that traditional organo-polyelectrolytes cannot. Metal cations also possess unusual physiochemical stability, which could be well utilized in applications requiring robust ion exchange and transport. Given these rich properties, we summarize below specific applications and challenges, and provide future perspectives of metallo-polyelectrolytes.

The application of metallo-polyelectrolytes for polyelectrolyte multilayers not only enables emerging methods of assembly, but also introduces multiple functions and responsiveness. With the abundant choices of transition metal elements from the Periodic Table, it is believed that metallo-polyelectrolyte-based PEMs could find more applications from functional coatings to biomedical utilities. The redox properties of metals allow controlled disassembly, swelling, electrochromism, etc. On the other hand, metallo-polyelectrolytes have shown some superior antimicrobial activities due to synergistic mechanisms of action, which combine the intrinsic ability of metal ions and unexpected efficiency of ionic complexation. Benefited from the versatile methodology of macromolecular synthesis, metal moieties can be conjugated into polymeric scaffolds in a more refined manner. It is envisioned that metallo-polyelectrolytes have great potential in fighting against multi-drug resistant bacteria. Metallo-polyelectrolytes can be also a promising non-viral vector candidate for efficient gene delivery, though most research is still at the early infancy stage. Many traditional amino-based polymers suffer from significant toxicity and instability of polyplexes with nucleic acids under physiological environments. In comparison, a few metallo-polyelectrolytes exhibit robust chemical stability toward harsh conditions and nontoxicity for mammalian cells. Finally, cationic metallo-polyelectrolytes have been utilized for ion exchange and transport, particularly for hydroxide ions in electrochemical devices. They are promising materials for the next-generation anion-exchange membranes. There have been no reports on cell performance on these metal-containing membranes yet. Further improvement on alkaline and chemical stability and ionic conductivity is needed. A highly stable metal cation is necessary, but insufficient. More stable polymer backbones and linkages are also essential.

However, there exists significant challenges and hurdles facing metallo-polyelectrolytes, which need to be addressed in order to achieve greater emerging properties and applications:

Quantitative analysis of ionic binding strength of metallo-polyelectrolytes with molecular substrates is largely missing. While similar dilemma is faced with the entire field of polyelectrolytes, our understanding on metallo-polyelectrolytes is almost exclusively qualitative. For organo-polyelectrolytes, quantitative interaction strength of PECs has been directly probed by the ability of salts added to break ion pairs using spectroscopic methods. The Schlenoff group have pioneered on the salt-water plasticization of PECs (“saloplasticity”) to extrude dense shapes suitable for many types of measurements, including ion equilibria29,157. However, quantitative binding with small molecules or membranes is critically needed. An establishment of representative phase diagram would be a challenging, but worthy task to explore158. There is an urgent need to establish quantitative physical chemistry of metallo-polyelectrolytes.

Theoretical models are needed to construct and predict key influential parameters on polyelectrolyte complexation28,159. These models could combine with experimental analysis to augment the understanding at molecular and macroscopic levels. A mean-field model of complex coacervation from weakly charged polymers has been established by Voorn and Overbeek160. Parameters include chain length, charge density and concentrations of polymers and salts. Random phase approximation allows high charge densities and connectivity of polymer segments, as studied in early 2000161,162. Later, an off-lattice approach describing polyelectrolyte complexation for polyelectrolytes with pH-dependent charges was constructed by Biesheuvel and Cohen Stuart163. Very recently, a coarse-grained model is proposed to simulate coacervation as a function of polymer length and overall salt concentration by the de Palbo group164. These models could help estimate the total free energy of mixing as well as mixing entropy and enthalpy terms. However, these models only deal with organo-polyelectrolytes. New models or advanced versions of the above models must take consideration of metal cations and their unique electronic and redox properties.

Grand challenges would be dealing with more complex biological interfaces. For example, multiple mechanisms of action have been proposed for antimicrobial activity of metallo-polyelectrolytes. However, the intrinsic free energy change such as enthalpy and entropy change as well as solubility equilibrium, which ultimately interferes key biochemical processes, is still not quantitively studied. A more comprehensive understanding is needed to direct the design of macromolecular metal complexes, i.e., macromolecular structures, complex types and metal moieties. Another example involves ion transport. Theoretical modelling could help design new metallo-polyelectrolytes with better phase-separated structures for improved electrochemical performance.

Nevertheless, the fields of polymer and macromolecular sciences have overseen the enormous potential of metallo-polyelectrolytes for a broad range of fundamental scientific mysteries and practical applications. Much work on the chemistry, biology, physics and engineering of metallo-polyelectrolytes is expected in the coming years to fulfill the ever-increasing demands for functional advanced materials.


  1. 1.

    Hara, M. Polyelectrolytes: Science and Technology (CRC Press, 1992).

  2. 2.

    Hiller, J. A., Mendelsohn, J. D. & Rubner, M. F. Reversibly Erasable Nanoporous Anti-Reflection Coatings from Polyelectrolyte Multilayers. Nat. Mater. 1, 59–63 (2002).

    ADS  CAS  PubMed  Google Scholar 

  3. 3.

    Pack, D. W., Hoffman, A. S., Pun, S. & Stayton, P. S. Design and Development of Polymers for Gene Delivery. Nat. Rev. Drug. Discov. 4, 581–593 (2005).

    CAS  PubMed  Google Scholar 

  4. 4.

    Viovy, J.-L. Electrophoresis of DNA and Other Polyelectrolytes: Physical Mechanisms. Rev. Mod. Phys. 72, 813 (2000).

    ADS  CAS  Google Scholar 

  5. 5.

    Dobrynin, A. V. & Rubinstein, M. Theory of Polyelectrolytes in Solutions and at Surfaces. Prog. Polym. Sci. 30, 1049–1118 (2005).

    CAS  Google Scholar 

  6. 6.

    Jiang, H., Taranekar, P., Reynolds, J. R. & Schanze, K. S. Conjugated Polyelectrolytes: Synthesis, Photophysics, and Applications. Angew. Chem. Int. Ed. 48, 4300–4316 (2009).

    CAS  Google Scholar 

  7. 7.

    Mecerreyes, D. Polymeric Ionic Liquids: Broadening the Properties and Applications of Polyelectrolytes. Prog. Polym. Sci. 36, 1629–1648 (2011).

    CAS  Google Scholar 

  8. 8.

    Yan, Y., Zhang, J., Ren, L. & Tang, C. Metal-Containing and Related Polymers for Biomedical Applications. Chem. Soc. Rev. 45, 5232–5263 (2016).

    CAS  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Whittell, G. R., Hager, M. D., Schubert, U. S. & Manners, I. Functional Soft Materials from Metallopolymers and Metallosupramolecular Polymers. Nat. Mater. 10, 176–188 (2011).

    ADS  CAS  PubMed  Google Scholar 

  10. 10.

    Beck, J. B. & Rowan, S. J. Multistimuli, Multiresponsive Metallo-Supramolecular Polymers. J. Am. Chem. Soc. 125, 13922–13923 (2003).

    CAS  PubMed  Google Scholar 

  11. 11.

    Williams, K. A., Boydston, A. J. & Bielawski, C. W. Main-Chain Organometallic Polymers: Synthetic Strategies, Applications, and Perspectives. Chem. Soc. Rev. 36, 729–744 (2007).

    CAS  PubMed  Google Scholar 

  12. 12.

    Hailes, R. L. N., Oliver, A. M., Gwyther, J., Whittell, G. R. & Manners, I. Polyferrocenylsilanes: Synthesis, Properties, and Applications. Chem. Soc. Rev. 45, 5358–5407 (2016).

    CAS  PubMed  Google Scholar 

  13. 13.

    Zhao, L. et al. Metallomacromolecules Containing Cobalt Sandwich Complexes: Synthesis and Functional Materials Properties. Coord. Chem. Rev. 337, 34–79 (2017).

    CAS  Google Scholar 

  14. 14.

    Hardy, C. G., Zhang, J., Yan, Y., Ren, L. & Tang, C. Metallopolymers with Transition Metals in the Side-Chain by Living and Controlled Polymerization Techniques. Prog. Polym. Sci. 39, 1742–1796 (2014).

    CAS  Google Scholar 

  15. 15.

    Zhang, K. Y., Liu, S., Zhao, Q. & Huang, W. Stimuli–Responsive Metallopolymers. Coord. Chem. Rev. 319, 180–195 (2016).

    CAS  Google Scholar 

  16. 16.

    Philipp, B., Dautzenberg, H., Linow, K.-J., Kötz, J. & Dawydoff, W. Polyelectrolyte Complexes—Recent Developments and Open Problems. Prog. Polym. Sci. 14, 91–172 (1989).

    CAS  Google Scholar 

  17. 17.

    Thünemann, A. F., Müller, M., Dautzenberg, H., Joanny, J.-F., Löwen, H. Polyelectrolyte Complexes. In: Polyelectrolytes with Defined Molecular Architecture II (ed. Schmidt, M.) (Springer, 2004).

  18. 18.

    Wang, Q. & Schlenoff, J. B. The Polyelectrolyte Complex/Coacervate Continuum. Macromolecules 47, 3108–3116 (2014).

    ADS  CAS  Google Scholar 

  19. 19.

    Chollakup, R., Smitthipong, W., Eisenbach, C. D. & Tirrell, M. Phase Behavior and Coacervation of Aqueous Poly(Acrylic Acid)−Poly(Allylamine) Solutions. Macromolecules 43, 2518–2528 (2010).

    ADS  CAS  Google Scholar 

  20. 20.

    Pageni, P., Kabir, M. P., Yang, P. & Tang, C. Binding of Cobaltocenium-Containing Polyelectrolytes with Anionic Probes. J. Inorg. Organomet. Polym. Mater. 27, 1100–1109 (2017).

    CAS  PubMed  PubMed Central  Google Scholar 

  21. 21.

    Ma, Y., Dong, W. F., Hempenius, M. A., Möhwald, H. & Vancso, G. J. Redox-Controlled Molecular Permeability of Composite-Wall Microcapsules. Nat. Mater. 5, 724–729 (2006). An important paper reports redox-responsive ferrocene-containing polyelectrolytes.

    ADS  CAS  PubMed  Google Scholar 

  22. 22.

    Wang, S. et al. Warm/Cool-Tone Switchable Thermochromic Material for Smart Windows by Orthogonally Integrating Properties of Pillar [6] Arene and Ferrocene. Nat. Commun. 9, 1737 (2018).

    ADS  PubMed  PubMed Central  Google Scholar 

  23. 23.

    Zha, Y., Disabb-Miller, M. L., Johnson, Z. D., Hickner, M. A. & Tew, G. N. Metal-Cation-Based Anion Exchange Membranes. J. Am. Chem. Soc. 134, 4493–4496 (2012). The first paper to report metallo-polyelectrolytes for ion-exchange application.

    CAS  PubMed  Google Scholar 

  24. 24.

    Zhu, T. et al. Cationic Metallo‐Polyelectrolytes for Robust Alkaline Anion‐Exchange Membranes. Angew. Chem. Int. Ed. 57, 2388–2392 (2018).

    CAS  Google Scholar 

  25. 25.

    Pearson, R. G. Hard and Soft Acids and Bases. J. Am. Chem. Soc. 85, 3533–3539 (1963).

    CAS  Google Scholar 

  26. 26.

    Pearson, R. G. Hard and Soft Acids and Bases, Hsab, Part II: Underlying Theories. J. Chem. Educ. 45, 643 (1968).

    CAS  Google Scholar 

  27. 27.

    Pearson, R. G. Recent Advances in the Concept of Hard and Soft Acids and Bases. J. Chem. Educ. 64, 561 (1987).

    CAS  Google Scholar 

  28. 28.

    Van der Gucht, J., Spruijt, E., Lemmers, M. & Stuart, M. A. C. Polyelectrolyte Complexes: Bulk Phases and Colloidal Systems. J. Colloid Interface Sci. 361, 407–422 (2011). A critical review to describe the formation of polyelectrolyte complexes.

    ADS  PubMed  Google Scholar 

  29. 29.

    Fu, J., Fares, H. M. & Schlenoff, J. B. Ion-Pairing Strength in Polyelectrolyte Complexes. Macromolecules 50, 1066–1074 (2017).

    ADS  CAS  Google Scholar 

  30. 30.

    Zhang, J. Y. et al. Charged Metallopolymers as Universal Precursors for Versatile Cobalt Materials. Angew. Chem. Int. Ed. 52, 13387–13391 (2013).

    ADS  CAS  Google Scholar 

  31. 31.

    Zhang, J. Y. et al. Antimicrobial Metallopolymers and Their Bioconjugates with Conventional Antibiotics against Multidrug-Resistant Bacteria. J. Am. Chem. Soc. 136, 4873–4876 (2014). This work reports bioconjugates between metallo-polyelectrolytes and antibiotics.

    CAS  PubMed  Google Scholar 

  32. 32.

    Bucur, C. B., Sui, Z. & Schlenoff, J. B. Ideal Mixing in Polyelectrolyte Complexes and Multilayers: Entropy Driven Assembly. J. Am. Chem. Soc. 128, 13690–13691 (2006).

    CAS  PubMed  Google Scholar 

  33. 33.

    Chollakup, R., Beck, J. B., Dirnberger, K., Tirrell, M. & Eisenbach, C. D. Polyelectrolyte Molecular Weight and Salt Effects on the Phase Behavior and Coacervation of Aqueous Solutions of Poly(Acrylic Acid) Sodium Salt and Poly(Allylamine) Hydrochloride. Macromolecules 46, 2376–2390 (2013).

    ADS  CAS  Google Scholar 

  34. 34.

    Fares, H. M. & Schlenoff, J. B. Diffusion of Sites Versus Polymers in Polyelectrolyte Complexes and Multilayers. J. Am. Chem. Soc. 139, 14656–14667 (2017). This publication reports the mass transport of polymers and molecules in the PEMs.

    CAS  PubMed  Google Scholar 

  35. 35.

    Flanagan, J. B., Margel, S., Bard, A. J. & Anson, F. C. Electron Transfer to and from Molecules Containing Multiple, Noninteracting Redox Centers. Electrochemical Oxidation of Poly(Vinylferrocene). J. Am. Chem. Soc. 100, 4248–4253 (1978).

    CAS  Google Scholar 

  36. 36.

    Denisevich, P., Willman, K. W. & Murray, R. W. Unidirectional Current Flow and Charge State Trapping at Redox Polymer Interfaces on Bilayer Electrodes: Principles, Experimental Demonstration, and Theory. J. Am. Chem. Soc. 103, 4727–4737 (1981).

    CAS  Google Scholar 

  37. 37.

    Chidesy, C. E. D. & Murray, R. W. Electroactive Polymers and Macromolecular Electronics. Science 231, 25–31 (1986).

    ADS  Google Scholar 

  38. 38.

    Willner, I., Riklin, A. & Lapidot, N. Electron-Transfer Communication between a Redox Polymer Matrix and an Immobilized Enzyme: Activity of Nitrate Reductase in a Viologen-Acrylamide Copolymer. J. Am. Chem. Soc. 112, 6438–6439 (1990).

    CAS  Google Scholar 

  39. 39.

    Anson, F. C., Saveant, J. M. & Shigehara, K. New Model for the Interior of Polyelectrolyte Coatings on Electrode Surfaces. Mechanisms of Charge Transport through Protonated Poly(L-Lysine) Films Containing FeIII(edta)- and FeII(edta)2- as Counterions. J. Am. Chem. Soc. 105, 1096–1106 (1983).

    CAS  Google Scholar 

  40. 40.

    Methenitis, C., Morcellet-Sauvage, J. & Morcellet, M. The Interaction of Poly (N-Methacryloyl-L-Alanine) with Copper (II). Polym. Bull. 12, 133–139 (1984).

    CAS  Google Scholar 

  41. 41.

    Heller, A. Electrical Wiring of Redox Enzymes. Acc. Chem. Res. 23, 128–134 (1990).

    CAS  Google Scholar 

  42. 42.

    Degani, Y. & Heller, A. Electrical Communication between Redox Centers of Glucose Oxidase and Electrodes Via Electrostatically and Covalently Bound Redox Polymers. J. Am. Chem. Soc. 111, 2357–2358 (1989).

    CAS  Google Scholar 

  43. 43.

    Connelly, N. G. & Geiger, W. E. Chemical Redox Agents for Organometallic Chemistry. Chem. Rev. 96, 877–910 (1996).

    CAS  PubMed  Google Scholar 

  44. 44.

    Wang, Y., Rapakousiou, A. & Astruc, D. Romp Synthesis of Cobalticenium–Enamine Polyelectrolytes. Macromolecules 47, 3767–3774 (2014).

    ADS  CAS  Google Scholar 

  45. 45.

    Kazutake, T. et al. Cobaltocenium-Functionalized Poly(Propylene Imine) Dendrimers: Redox and Electromicrogravimetric Studies and Afm Imaging. Chem. Eur. J. 7, 1109–1117 (2001).

    Google Scholar 

  46. 46.

    Gu, H. et al. Tetrablock Metallopolymer Electrochromes. Angew. Chem. Int. Ed. 57, 2204–2208 (2018).

    CAS  Google Scholar 

  47. 47.

    Casado, C. M. et al. Mixed Ferrocene–Cobaltocenium Dendrimers: The Most Stable Organometallic Redox Systems Combined in a Dendritic Molecule. Angew. Chem. 112, 2219–2222 (2000).

    Google Scholar 

  48. 48.

    Cuadrado, I. et al. Preparation and Redox Properties of Novel Polymerizable Pyrrole- and Allyl-Functionalized Cobaltocenium Monomers and Siloxane-Based Cobaltocenium Polymers. Organometallics 18, 4960–4969 (1999).

    CAS  Google Scholar 

  49. 49.

    Staff, R. H. et al. Patchy Nanocapsules of Poly(Vinylferrocene)-Based Block Copolymers for Redox-Responsive Release. ACS Nano 6, 9042–9049 (2012).

    CAS  PubMed  Google Scholar 

  50. 50.

    Daniel, S., Moritz, vdL. & Markus, G. Synthesis of Breathing Metallopolymer Hollow Spheres for Redox-Controlled Release. Macromol. Rapid Commun. 37, 1573–1580 (2016).

    Google Scholar 

  51. 51.

    Rabiee Kenaree, A. & Gilroy, J. B. Synthesis and Characterization of Metal-Rich Phosphonium Polyelectrolytes and Their Use as Precursors to Nanomaterials. Dalton. Trans. 45, 18229–18240 (2016).

    CAS  PubMed  Google Scholar 

  52. 52.

    Apel, K. & Hirt, H. Reactive Oxygen Species: Metabolism, Oxidative Stress, and Signal Transduction. Annu. Rev. Plant. Biol. 55, 373–399 (2004).

    CAS  PubMed  Google Scholar 

  53. 53.

    Yakes, F. M. & Van Houten, B. Mitochondrial DNA Damage Is More Extensive and Persists Longer Than Nuclear DNA Damage in Human Cells Following Oxidative Stress. Proc. Natl. Acad. Sci. USA 94, 514–519 (1997).

    ADS  CAS  Google Scholar 

  54. 54.

    Hayyan, M., Hashim, M. A. & AlNashef, I. M. Superoxide Ion: Generation and Chemical Implications. Chem. Rev. 116, 3029–3085 (2016).

    CAS  PubMed  Google Scholar 

  55. 55.

    Lemire, J. A., Harrison, J. J. & Turner, R. J. Antimicrobial Activity of Metals: Mechanisms, Molecular Targets and Applications. Nat. Rev. Microbiol. 11, 371–384 (2013).

    CAS  PubMed  Google Scholar 

  56. 56.

    Abd-El-Aziz, A. S., Agatemor, C. & Etkin, N. Antimicrobial Resistance Challenged with Metal-Based Antimicrobial Macromolecules. Biomaterials 118, 27–50 (2017).

    CAS  PubMed  Google Scholar 

  57. 57.

    Tang, Z., Wang, Y., Podsiadlo, P. & Kotov, N. A. Biomedical Applications of Layer‐by‐Layer Assembly: From Biomimetics to Tissue Engineering. Adv. Mater. 18, 3203–3224 (2006).

    CAS  Google Scholar 

  58. 58.

    Zhang, X., Chen, H., Zhang, H. Layer-by-Layer Assembly: From Conventional to Unconventional Methods. Chem Commun. 0, 1395–1405 (2007).

  59. 59.

    Hammond, P. T. Form and Function in Multilayer Assembly: New Applications at the Nanoscale. Adv. Mater. 16, 1271–1293 (2004).

    CAS  Google Scholar 

  60. 60.

    Dubas, S. T. & Schlenoff, J. B. Factors Controlling the Growth of Polyelectrolyte Multilayers. Macromolecules 32, 8153–8160 (1999).

    ADS  CAS  Google Scholar 

  61. 61.

    Schönhoff, M. Self-Assembled Polyelectrolyte Multilayers. Curr. Opin. Colloid Interface Sci. 8, 86–95 (2004).

    Google Scholar 

  62. 62.

    Séon, L., Lavalle, P., Schaaf, P. & Boulmedais, F. Polyelectrolyte Multilayers: A Versatile Tool for Preparing Antimicrobial Coatings. Langmuir 31, 12856–12872 (2015).

    PubMed  Google Scholar 

  63. 63.

    Boudou, T., Crouzier, T., Ren, K. F., Blin, G. & Picart, C. Multiple Functionalities of Polyelectrolyte Multilayer Films: New Biomedical Applications. Adv. Mater. 22, 441–467 (2010).

    CAS  PubMed  Google Scholar 

  64. 64.

    Stuart, M. A. C. et al. Emerging Applications of Stimuli-Responsive Polymer Materials. Nat. Mater. 9, 101–113 (2010).

    ADS  PubMed  Google Scholar 

  65. 65.

    Wei, J., Ren, L., Tang, C. & Su, Z. Electric-Stimulus-Responsive Multilayer Films Based on a Cobaltocenium-Containing Polymer. Polym. Chem. 5, 6480–6488 (2014).

    CAS  Google Scholar 

  66. 66.

    Tagliazucchi, M., Grumelli, D. & Calvo, E. J. Nanostructured Modified Electrodes: Role of Ions and Solvent Flux in Redox Active Polyelectrolyte Multilayer Films. Phys. Chem. Chem. Phys. 8, 5086–5095 (2006).

    CAS  PubMed  Google Scholar 

  67. 67.

    Zahn, R., Boulmedais, F., Vörös, J. N., Schaaf, P. & Zambelli, T. Ion and Solvent Exchange Processes in Pga/Pah Polyelectrolyte Multilayers Containing Ferrocyanide. J. Phys. Chem. B 114, 3759–3768 (2010).

    CAS  PubMed  Google Scholar 

  68. 68.

    Bernards, D. A. & Desai, T. A. Nanoscale Porosity in Polymer Films: Fabrication and Therapeutic Applications. Soft Matter 6, 1621–1631 (2010).

    ADS  CAS  PubMed  PubMed Central  Google Scholar 

  69. 69.

    Overbeek, J. T. The Donnan Equilibrium. Prog. Biophys. Biophys. Chem. 6, 57–84 (1956).

    CAS  PubMed  Google Scholar 

  70. 70.

    Calvo, E. J., Forzani, E. & Otero, M. Gravimetric and Viscoelastic Changes During the Oxidation–Reduction of Layer-by-Layer Self Assembled Enzyme Multilayers Wired by an Os-Containing Poly(Allylamine) Polymer. J. Electroanal. Chem. 538, 231–241 (2002).

    Google Scholar 

  71. 71.

    Tagliazucchi, M., Williams, F. J. & Calvo, E. J. Metal-Ion Responsive Redox Polyelectrolyte Multilayers. Chem. Commun. 46, 9004–9006 (2010).

    CAS  Google Scholar 

  72. 72.

    Rydzek, G. et al. Electrochemical Nanoarchitectonics and Layer-by-Layer Assembly: From Basics to Future. Nano Today 10, 138–167 (2015).

    CAS  Google Scholar 

  73. 73.

    Rahim, M. A. et al. Metal Ion-Enriched Polyelectrolyte Complexes and Their Utilization in Multilayer Assembly and Catalytic Nanocomposite Films. Langmuir 28, 8486–8495 (2012).

    CAS  PubMed  Google Scholar 

  74. 74.

    Liang, Y. et al. Soft Coordination Supramolecular Polymers: Novel Materials for Dual Electro-Catalysis. RSC Adv. 2, 12732–12738 (2012).

    CAS  Google Scholar 

  75. 75.

    Miao, W.-K. et al. Incorporation of Polyoxometalates into Polymers to Create Linear Poly (Polyoxometalate)S with Catalytic Function. ACS Macro Lett. 3, 211–215 (2014).

    CAS  Google Scholar 

  76. 76.

    Kurth, D. G., Pitarch Lopez, J., Dong, W.-F. A New Co(II)-Metalloviologen-Based Electrochromic Material Integrated in Thin Multilayer Films. Chem Commun. 0,2119–2121 (2005).

  77. 77.

    Maier, A., Rabindranath, A. R. & Tieke, B. Coordinative supramolecular assembly of electrochromic films based on metal ion complexes of polyiminofluorene with terpyridine substituent groups. Chem. Mater. 23, 4084–4084 (2011).

    CAS  Google Scholar 

  78. 78.

    Tieke, B. Coordinative supramolecular assembly of electrochromic thin films. Curr. Opin. Colloid Interface Sci 16, 499–507 (2011).

    CAS  Google Scholar 

  79. 79.

    Zhang, C. F. et al. Interfacial self-assembly of metal-mediated viologen-like coordination polyelectrolyte hybrids of the bisterpyridine ligand and their optical, electrochemical, and electrochromic properties. ACS Appl. Mater. Interfaces 1, 1250–1258 (2009).

    CAS  Google Scholar 

  80. 80.

    Han, F. S., Higuchi, M. & Kurth, D. G. Metallo‐supramolecular polymers based on functionalized bis‐terpyridines as novel electrochromic materials. Adv. Mater. 19, 3928–3931 (2007). The first paper to propose the structure-property relationship of electrochromic properties of metallo-supramolecular coordination polyelectrolytes.

    CAS  Google Scholar 

  81. 81.

    Schwarz, G. et al. Tuning the structure and the magnetic properties of metallo-supramolecular polyelectrolyte-amphiphile complexes. J. Am. Chem. Soc. 133, 547–558 (2011).

    CAS  PubMed  Google Scholar 

  82. 82.

    Bodenthin, Y., Pietsch, U., Möhwald, H. & Kurth, D. G. Inducing spin crossover in metallo-supramolecular polyelectrolytes through an amphiphilic phase transition. J. Am. Chem. Soc. 127, 3110–3114 (2005).

    CAS  PubMed  Google Scholar 

  83. 83.

    Yan, Y. et al. Redox responsive molecular assemblies based on metallic coordination polymers. Soft Matter 6, 3244–3248 (2010).

    ADS  CAS  Google Scholar 

  84. 84.

    Hadadpour, M., Gwyther, J., Manners, I. & Ragogna, P. J. Multifunctional block copolymer: where polymetallic and polyelectrolyte blocks meet. Chem. Mater. 27, 3430–3440 (2015).

    CAS  Google Scholar 

  85. 85.

    Rabiee Kenaree, A., Berven, B. M., Ragogna, P. J. & Gilroy, J. B. Highly-metallized phosphonium polyelectrolytes. Chem. Commun. 50, 10714–10717 (2014).

    CAS  Google Scholar 

  86. 86.

    Burnworth, M. et al. Optically healable supramolecular polymers. Nature 472, 334–337 (2011).

    ADS  CAS  PubMed  Google Scholar 

  87. 87.

    Wang, S., Iv, C. J. Z., Jiang, J., Pan, Z., Schanze, K. S. Intercalation of Alkynylplatinum(II) Terpyridine Complexes into a Helical Poly(Phenylene Ethynylene) Sulfonate: Application to Protein Sensing. ACS Appl. Mater. Interfaces 9, 33461–33469 (2017).

    CAS  Google Scholar 

  88. 88.

    Chung, C. Y.-S. & Yam, V. W.-W. Induced self-assembly and förster resonance energy transfer studies of Alkynylplatinum(II) terpyridine complex through interaction with water-soluble Poly(Phenylene Ethynylene Sulfonate) and the proof-of-principle demonstration of this two-component ensemble for selective label-free detection of human serum albumin. J. Am. Chem. Soc. 133, 18775–18784 (2011).

    CAS  PubMed  Google Scholar 

  89. 89.

    Ulman, A. Formation and structure of self-assembled monolayers. Chem. Rev. 96, 1533–1554 (1996).

    CAS  PubMed  Google Scholar 

  90. 90.

    Laws, D. R., Sheats, J., Rheingold, A. L. & Geiger, W. E. Organometallic electrodes: modification of electrode surfaces through cathodic reduction of cyclopentadienyldiazonium complexes of cobalt and manganese. Langmuir 26, 15010–15021 (2010).

    CAS  PubMed  Google Scholar 

  91. 91.

    Sheridan, M. V., Lam, K. & Geiger, W. E. An anodic method for covalent attachment of molecules to electrodes through an ethynyl linkage. J. Am. Chem. Soc. 135, 2939–2942 (2013).

    CAS  PubMed  Google Scholar 

  92. 92.

    Tagliazucchi, M., Calvo, E. J. & Szleifer, I. Molecular theory of chemically modified electrodes by redox polyelectrolytes under equilibrium conditions: comparison with experiment. J. Phys. Chem. C. 112, 458–471 (2008).

    CAS  Google Scholar 

  93. 93.

    Gummel, J., Cousin, F. & Boué, F. Counterions release from electrostatic complexes of polyelectrolytes and proteins of opposite charge: a direct measurement. J. Am. Chem. Soc. 129, 5806–5807 (2007).

    CAS  PubMed  Google Scholar 

  94. 94.

    Wallin, T. & Linse, P. Monte Carlo simulations of polyelectrolytes at charged micelles. 1. Effects of chain flexibility. Langmuir 12, 305–314 (1996).

    CAS  Google Scholar 

  95. 95.

    Brown, E. D. & Wright, G. D. Antibacterial drug discovery in the resistance era. Nature 529, 336–343 (2016).

    ADS  CAS  PubMed  Google Scholar 

  96. 96.

    Ganewatta, M. S. & Tang, C. Controlling macromolecular structures towards effective antimicrobial polymers. Polymer 63, A1–A29 (2015).

    CAS  Google Scholar 

  97. 97.

    Tew, G. N. et al. De Novo design of biomimetic antimicrobial polymers. Proc. Natl Acad. Sci. USA 99, 5110–5114 (2002).

    ADS  CAS  PubMed  Google Scholar 

  98. 98.

    Engler, A. C. et al. Emerging trends in macromolecular antimicrobials to fight multi-drug-resistant infections. Nano Today 7, 201–222 (2012).

    CAS  Google Scholar 

  99. 99.

    Ergene, C., Yasuhara, K. & Palermo, E. F. Biomimetic antimicrobial polymers: recent advances in molecular design. Polym. Chem. 9, 2407–2427 (2018).

    CAS  Google Scholar 

  100. 100.

    Regiel-Futyra, A. et al. Bioinorganic antimicrobial strategies in the resistance era. Coord. Chem. Rev. 351, 76–117 (2017).

    CAS  Google Scholar 

  101. 101.

    Rivas, B. L., Pereira, E. & Maureira, A. Functional water-soluble polymers: polymer-metal ion removal and biocide properties. Polym. Int. 58, 1093–1114 (2009).

    CAS  Google Scholar 

  102. 102.

    Gorle, A. K. et al. Mononuclear polypyridylruthenium(II) complexes with high membrane permeability in gram-negative bacteriain particular Pseudomonas aeruginosa. Chem. Eur. J. 21, 10472–10481 (2015).

    CAS  PubMed  Google Scholar 

  103. 103.

    Gorle, A. K. et al. Tri- and Tetra-Nuclear Polypyridyl Ruthenium(II) Complexes as Antimicrobial Agents. Dalton. Trans. 43, 16713–16725 (2014).

    CAS  PubMed  Google Scholar 

  104. 104.

    Li, F. F. et al. The antimicrobial activity of inert oligonuclear Polypyridylruthenium(II) complexes against Pathogenic Bacteria, including Mrsa. Dalton Trans. 40, 5032–5038 (2011).

    CAS  PubMed  Google Scholar 

  105. 105.

    Hassan, E. A., Hassan, M. L., Moorefield, C. N. & Newkome, G. R. New supramolecular metallo-terpyridine carboxymethyl cellulose derivatives with antimicrobial properties. Carbohydr. Polym. 116, 2–8 (2015).

    CAS  PubMed  Google Scholar 

  106. 106.

    Gasser, G., Ott, I. & Metzler-Nolte, N. Organometallic anticancer compounds. J. Med. Chem. 54, 3–25 (2010).

    PubMed  PubMed Central  Google Scholar 

  107. 107.

    Abd-El-Aziz, A. S., Abdelghani, A. A., El-Sadany, S. K., Overy, D. P. & Kerr, R. G. Antimicrobial and anticancer activities of organoiron melamine dendrimers capped with piperazine moieties. Eur. Polym. J. 82, 307–323 (2016).

    CAS  Google Scholar 

  108. 108.

    Abd-El-Aziz, A. S., Agatemor, C., Etkin, N., Overy, D. P. & Kerr, R. G. Redox-active cationic organoiron complex: a promising lead structure for developing antimicrobial agents with activity against gram-positive pathogens including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus Faecium. RSC Adv. 5, 86421–86427 (2015).

    CAS  Google Scholar 

  109. 109.

    Abd-El-Aziz, A. S. et al. Design of piperazine organoiron macromolecules with antibacterial and anticancer activity. Macromol. Chem. Phys. 217, 987–996 (2016).

    CAS  Google Scholar 

  110. 110.

    Abd-El-Aziz, A. S. et al. Antimicrobial organometallic dendrimers with tunable activity against multidrug-resistant bacteria. Biomacromolecules 16, 3694–3703 (2015).

    CAS  PubMed  Google Scholar 

  111. 111.

    Park, S.-C. et al. Polymeric micellar nanoplatforms for fenton reaction as a new class of antibacterial agents. J. Control Release 221, 37–47 (2016).

    CAS  PubMed  Google Scholar 

  112. 112.

    Pierre, J. & Fontecave, M. Iron and activated oxygen species in biology: the basic chemistry. Biometals 12, 195–199 (1999).

    CAS  PubMed  Google Scholar 

  113. 113.

    Joyner, J. C. et al. Antimicrobial metallopeptides with broad nuclease and ribonuclease activity. Chem. Commun. 49, 2118–2120 (2013).

    CAS  Google Scholar 

  114. 114.

    Chantson, J. T., Falzacappa, M. V. V., Crovella, S. & Metzler-Nolte, N. Solid-phase synthesis, characterization, and antibacterial activities of metallocene-peptide bioconjugates. ChemMedChem 1, 1268–1274 (2006).

    CAS  PubMed  Google Scholar 

  115. 115.

    Noor, F., Wustholz, A., Kinscherf, R. & Metzler-Nolte, N. A Cobaltocenium-Peptide Bioconjugate Shows Enhanced Cellular Uptake and Directed Nuclear Delivery. Angew. Chem. Int Ed. 44, 2429–2432 (2005). The first paper reports the use of cobaltocenium for gene delivery.

    CAS  Google Scholar 

  116. 116.

    Chantson, J. T., Falzacappa, M. V. V., Crovella, S. & Metzler-Nolte, N. Antibacterial activities of ferrocenoyl- and cobaltocenium-peptide bioconjugates. J. Organomet. Chem. 690, 4564–4572 (2005).

    CAS  Google Scholar 

  117. 117.

    Yang, P. et al. Trio act of boronolectin with antibiotic-metal complexed macromolecules toward broad-spectrum antimicrobial efficacy. ACS Infect. Dis. 3, 845–853 (2017).

    CAS  PubMed  PubMed Central  Google Scholar 

  118. 118.

    Zhang, J. et al. Anion-responsive metallopolymer hydrogels for healthcare applications. Sci. Rep. 5, 11914 (2015).

    ADS  CAS  PubMed  PubMed Central  Google Scholar 

  119. 119.

    Takahashi, H., Caputo, G. A., Vemparala, S. & Kuroda, K. Synthetic random copolymers as a molecular platform to mimic host-defense antimicrobial peptides. Bioconjugate Chem. 28, 1340–1350 (2017).

    CAS  Google Scholar 

  120. 120.

    Lindhoud, S., Norde, W. & Stuart, M. A. C. Effects of polyelectrolyte complex micelles and their components on the enzymatic activity of lipase. Langmuir 26, 9802–9808 (2010).

    CAS  PubMed  Google Scholar 

  121. 121.

    Pageni, P. et al. Recyclable magnetic nanoparticles grafted with antimicrobial metallopolymer-antibiotic bioconjugates. Biomaterials 178, 363–372 (2018).

    CAS  PubMed  Google Scholar 

  122. 122.

    Pageni, P. et al. Charged metallopolymer-grafted silica nanoparticles for antimicrobial applications. Biomacromolecules 19, 417–425 (2018).

    CAS  PubMed  PubMed Central  Google Scholar 

  123. 123.

    Cavazzana-Calvo, M. et al. Gene therapy of human severe combined immunodeficiency (Scid)-X1 disease. Science 288, 669–672 (2000).

    ADS  CAS  PubMed  Google Scholar 

  124. 124.

    Yang, J. P., Zhang, Q., Chang, H. & Cheng, Y. Y. Surface-engineered dendrimers in gene delivery. Chem. Rev. 115, 5274–5300 (2015).

    CAS  PubMed  Google Scholar 

  125. 125.

    Yin, H. et al. Non-viral vectors for gene-based therapy. Nat. Rev. Genet. 15, 541–555 (2014).

    CAS  PubMed  Google Scholar 

  126. 126.

    Lachelt, U. & Wagner, E. Nucleic acid therapeutics using polyplexes: a journey of 50 Years (and Beyond). Chem. Rev. 115, 11043–11078 (2015).

    PubMed  Google Scholar 

  127. 127.

    van Staveren, D. R. & Metzler-Nolte, N. Bioorganometallic chemistry of ferrocene. Chem. Rev. 104, 5931–5985 (2004).

    PubMed  Google Scholar 

  128. 128.

    Qiu, H. B., Gilroy, J. B. & Manners, I. DNA-induced chirality in water-soluble Poly(Cobaltoceniumethylene). Chem. Commun. 49, 42–44 (2013).

    CAS  Google Scholar 

  129. 129.

    Mayer, U. F. J., Gilroy, J. B., O’Hare, D. & Manners, I. Ring-Opening Polymerization of 19-Electron [2]Cobaltocenophanes: A Route to High-Molecular-Weight, Water-Soluble Polycobaltocenium Polyelectrolytes. J. Am. Chem. Soc. 131, 10382–10383 (2009).

    CAS  PubMed  Google Scholar 

  130. 130.

    Gilroy, J. B., Patra, S. K., Mitchels, J. M., Winnik, M. A. & Manners, I. Main‐chain heterobimetallic block copolymers: synthesis and self‐assembly of polyferrocenylsilane‐b‐Poly (Cobaltoceniumethylene). Angew. Chem. Int. Ed. 50, 5851–5855 (2011).

    CAS  Google Scholar 

  131. 131.

    Musgrave, R. A. et al. Chiral Transmission to Cationic Polycobaltocenes over Multiple Length Scales Using Anionic Surfactants. J. Am. Chem. Soc. 140, 7222–7231 (2018). This work describes chiral complexes of metallo-polyelectrolytes with chiral surfactant counteranions.

    CAS  PubMed  Google Scholar 

  132. 132.

    Cheng, Y. L., Yumul, R. C. & Pun, S. H. Virus-inspired polymer for efficient in vitro and in vivo gene delivery. Angew. Chem. Int. Ed. 55, 12013–12017 (2016).

    CAS  Google Scholar 

  133. 133.

    Ma, Y. J., Dong, W. F., Hempenius, M. A., Mohwald, H. & Vancso, G. J. Layer-by-layer constructed macroporous architectures. Angew. Chem. Int. Ed. 46, 1702–1705 (2007).

    CAS  Google Scholar 

  134. 134.

    Zhong, Z. Y. et al. Water-soluble cationic Poly(Ferrocenylsilane): an efficient DNA condensation and transfection agent. J. Control Release 116, 81–83 (2006).

    Google Scholar 

  135. 135.

    Varcoe, J. R. & Slade, R. C. Prospects for alkaline anion‐exchange membranes in low temperature fuel cells. Fuel Cells 5, 187–200 (2005).

    CAS  Google Scholar 

  136. 136.

    Couture, G., Alaaeddine, A., Boschet, F. & Ameduri, B. Polymeric materials as anion-exchange membranes for alkaline fuel cells. Prog. Polym. Sci. 36, 1521–1557 (2011).

    CAS  Google Scholar 

  137. 137.

    Li, X., Zhang, H., Mai, Z., Zhang, H. & Vankelecom, I. Ion exchange membranes for vanadium redox flow battery (Vrb) applications. Energy Environ. Sci. 4, 1147–1160 (2011).

    CAS  Google Scholar 

  138. 138.

    Xu, T. Ion exchange membranes: state of their development and perspective. J. Membr. Sci. 263, 1–29 (2005).

    CAS  Google Scholar 

  139. 139.

    Robertson, N. J. et al. Tunable high performance cross-linked alkaline anion exchange membranes for fuel cell applications. J. Am. Chem. Soc. 132, 3400–3404 (2010).

    CAS  PubMed  Google Scholar 

  140. 140.

    Clark, T. J. et al. A ring-opening metathesis polymerization route to alkaline anion exchange membranes: development of hydroxide-conducting thin films from an ammonium-functionalized monomer. J. Am. Chem. Soc. 131, 12888–12889 (2009).

    CAS  PubMed  Google Scholar 

  141. 141.

    Pham, T. H., Olsson, J. S. & Jannasch, P. N-spirocyclic quaternary ammonium ionenes for anion-exchange membranes. J. Am. Chem. Soc. 139, 2888–2891 (2017).

    CAS  PubMed  Google Scholar 

  142. 142.

    Hugar, K. M., Kostalik, H. A. IV & Coates, G. W. Imidazolium cations with exceptional alkaline stability: a systematic study of structure–stability relationships. J. Am. Chem. Soc. 137, 8730–8737 (2015).

    CAS  PubMed  Google Scholar 

  143. 143.

    Fan, J. et al. Cationic polyelectrolytes, stable in 10 M KOHaq at 100 °C. ACS Macro Lett. 6, 1089–1093 (2017).

    CAS  Google Scholar 

  144. 144.

    Noonan, K. J. et al. Phosphonium-functionalized polyethylene: a new class of base-stable alkaline anion exchange membranes. J. Am. Chem. Soc. 134, 18161–18164 (2012).

    CAS  PubMed  Google Scholar 

  145. 145.

    Gu, S. et al. Quaternary phosphonium‐based polymers as hydroxide exchange membranes. ChemSusChem 3, 555–558 (2010).

    CAS  PubMed  Google Scholar 

  146. 146.

    Sata, T., Yamane, Y. & Matsusaki, K. Preparation and properties of anion exchange membranes having pyridinium or pyridinium derivatives as anion exchange groups. J. Polym. Sci., Part A: Polym. Chem. 36, 49–58 (1998).

    ADS  CAS  Google Scholar 

  147. 147.

    Kim, D. S., Labouriau, A., Guiver, M. D. & Kim, Y. S. Guanidinium-functionalized anion exchange polymer electrolytes via activated fluorophenyl-amine reaction. Chem. Mater. 23, 3795–3797 (2011).

    CAS  Google Scholar 

  148. 148.

    Zhang, Q., Li, S. & Zhang, S. A novel guanidinium grafted Poly (Aryl Ether Sulfone) for high-performance hydroxide exchange membranes. Chem. Commun. 46, 7495–7497 (2010).

    CAS  Google Scholar 

  149. 149.

    Arges, C. G. & Ramani, V. Two-Dimensional NMR spectroscopy reveals cation-triggered backbone degradation in polysulfone-based anion exchange membranes. Proc. Natl Acad. Sci. USA 110, 2490–2495 (2013).

    ADS  CAS  PubMed  Google Scholar 

  150. 150.

    Chen, N. et al. Cobaltocenium-containing polybenzimidazole polymers for alkaline anion exchange membrane applications. Polym. Chem. 8, 1381–1392 (2017).

    CAS  Google Scholar 

  151. 151.

    Gu, S. et al. Permethyl Cobaltocenium (Cp*2Co+) as an ultra-stable cation for polymer hydroxide-exchange membranes. Sci. Rep. 5, 11668 (2015).

    ADS  CAS  PubMed  PubMed Central  Google Scholar 

  152. 152.

    Varcoe, J. R. et al. Anion-exchange membranes in electrochemical energy systems. Energy Environ. Sci. 7, 3135–3191 (2014). An important review to summarize the development of polyelectrolyte membranes for electrochemical systems.

    CAS  Google Scholar 

  153. 153.

    Kwasny, M. T., Zhu, L., Hickner, M. A. & Tew, G. N. Thermodynamics of counterion release is critical for anion exchange membrane conductivity. J. Am. Chem. Soc. 140, 7961–7969 (2018).

    CAS  PubMed  Google Scholar 

  154. 154.

    Kwasny, M. T., Zhu, L., Hickner, M. A. & Tew, G. N. Utilizing thiol–ene chemistry for crosslinked nickel cation‐based anion exchange membranes. J. Polym. Sci., Part A: Polym. Chem. 56, 328–339 (2018).

    ADS  CAS  Google Scholar 

  155. 155.

    Shin, D. W., Guiver, M. D. & Lee, Y. M. Hydrocarbon-based polymer electrolyte membranes: importance of morphology on ion transport and membrane stability. Chem. Rev. 117, 4759–4805 (2017).

    CAS  PubMed  Google Scholar 

  156. 156.

    Li, N., Yan, T., Li, Z., Thurn-Albrecht, T. & Binder, W. H. Comb-shaped polymers to enhance hydroxide transport in anion exchange membranes. Energy Environ. Sci. 5, 7888–7892 (2012).

    CAS  Google Scholar 

  157. 157.

    Shamoun, R. F., Hariri, H. H., Ghostine, R. A. & Schlenoff, J. B. Thermal transformations in extruded saloplastic polyelectrolyte complexes. Macromolecules 45, 9759–9767 (2012).

    ADS  CAS  Google Scholar 

  158. 158.

    Rumyantsev, A. M., Kramarenko, E. Y., Borisov, O. V. Microphase separation in complex coacervate due to incompatibility between polyanion and polycation. Macromolecules 51, 6587–6601 (2018).

    ADS  CAS  Google Scholar 

  159. 159.

    Spruijt, E., Westphal, A. H., Borst, J. W., Cohen Stuart, M. A. & van der Gucht, J. Binodal compositions of polyelectrolyte complexes. Macromolecules 43, 6476–6484 (2010).

    ADS  CAS  Google Scholar 

  160. 160.

    Overbeek, J. T. G. & Voorn, M. Phase separation in polyelectrolyte solutions. Theory of complex coacervation. J. Cell. Physiol. 49, 7–26 (1957).

    CAS  Google Scholar 

  161. 161.

    Castelnovo, M. & Joanny, J.-F. Formation of polyelectrolyte multilayers. Langmuir 16, 7524–7532 (2000).

    CAS  Google Scholar 

  162. 162.

    Kudlay, A., Ermoshkin, A. V. & Olvera de La Cruz, M. Complexation of oppositely charged polyelectrolytes: effect of ion pair formation. Macromolecules 37, 9231–9241 (2004).

    ADS  CAS  Google Scholar 

  163. 163.

    Biesheuvel, P. M. & Cohen Stuart, M. A. Electrostatic free energy of weakly charged macromolecules in solution and intermacromolecular complexes consisting of oppositely charged polymers. Langmuir 20, 2785–2791 (2004).

    CAS  PubMed  Google Scholar 

  164. 164.

    Andreev, M., Prabhu, V. M., Douglas, J. F., Tirrell, M., de Pablo, J. J. Complex coacervation in polyelectrolytes from a coarse-grained model. Macromolecules 51, 6717–6723 (2018).

    ADS  CAS  Google Scholar 

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C.T., T.Z. and Y.S. acknowledge support from the National Institutes of Health (#R01AI120987). Y.Y. thanks NSFC (21504068), Fundamental Research Funds for the Central Universities (3102017jc01001 and 3102018jcc040), Open Project of State Key Laboratory of Supramolecular Structure and Materials (sklssm201826) and the Key Laboratory of Green Pesticide and Agricultural Bioengineering (2017GDGP0101).

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T.Z. and Y.S. equally contributed. T.Z., Y.S., J.Y., P.P. and M.A.R. wrote the manuscript. Y.Y. and C.T. finalized for publication.

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Correspondence to Yi Yan or Chuanbing Tang.

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Zhu, T., Sha, Y., Yan, J. et al. Metallo-polyelectrolytes as a class of ionic macromolecules for functional materials. Nat Commun 9, 4329 (2018).

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