Fig. 10 | Nature Communications

Fig. 10

From: LOTUS domain protein MARF1 binds CCR4-NOT deadenylase complex to post-transcriptionally regulate gene expression in oocytes

Fig. 10

Models for MARF1 function in oocytes. a RRM of MARF1 binds cyclin A mRNA and the mLOTUS domains of MARF1 binds and recruits the CCR4-NOT deadenylase complex(es) to cyclin A mRNA to shorten its poly-A tail. b MARF1 reduces Cyclin A protein level in stages 12–14 oocytes. This leads to the protein expression of Cyclin B and Cyclin B3, which results in phosphorylation of CDK1 Tyr15. Tyr15-phosphorylated CDK1 phosphorylates many target proteins, which may promote posterior germplasm localization of Vasa and Aubergine. The proper protein phosphorylation and/or proper Vasa/Aub localization to germplasm are required for successful completion of meiosis and fertility of eggs. c Loss of function of MARF1 causes an increase in Cyclin A protein level, which results in decrease of Cyclin B and Cyclin B3 protein levels in late-stage oocytes. This leads to a reduced phosphorylation of CDK1 Tyr15 and therefore an altered phosphorylation pattern of CDK1 target proteins. This may cause loss of the posterior germplasm localization of Vasa and Aubergine. Meiosis cannot complete successfully and therefore eggs are sterile

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