Fig. 7 | Nature Communications

Fig. 7

From: Ancient and modern anticonvulsants act synergistically in a KCNQ potassium channel binding pocket

Fig. 7

IVA and MTX synergize with RTG to lock open KCNQ2/3. a Averaged traces showing effects of high-dose RTG, IVA, and MTX alone or in combination on KCNQ2/3 (n = 5–31). Voltage protocol upper inset. be Analysis of traces as in a: b peak current; c tail current; d G/Gmax; e current fold-change versus voltage; compounds and combinations color-coded as in a. n = 5–31. f Mean effects of low-dose 1 µM RTG versus 1 µM of each of RTG, IVA, and MTX (1 + 1 + 1) versus 1 µM RTG + 10 µM IVA + 10 µM MTX (1 + 10 + 10) on KCNQ2/3 tail currents and G/Gmax versus prepulse voltages; n = 8–13. g KCNQ2/3 current fold-increase versus voltage induced by compounds as indicated alone or in combination; n = 8–13. **p < 0.01. h KCNQ2/3 current fold-increase versus voltage induced by compounds as indicated alone or in combination; n = 8–13. **p < 0.01. All error bars indicate SEM. All comparisons by one-way ANOVA

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