Fig. 4 | Nature Communications

Fig. 4

From: The last-born daughter cell contributes to division orientation of Drosophila larval neuroblasts

Fig. 4

The NB/GMC interface presents specific characteristics. a Two successive divisions of NBs expressing the membrane marker PH::RFP (magenta) and the midbody marker Pavarotti::GFP (green). Yellow asterisk: previously born GMC, cyan asterisk: GMC formed at subsequent division, magenta asterisks: last-born GMC in this series. Blue arrowhead: midbody formed at the first division recorded stays at the newly formed NB/GMC interface but is inherited by the GMC in the next division, when a new midbody remains at the subsequently formed NB/GMC interphase (magenta arrowhead). b NB expressing the membrane marker Rap1::GFP. Tubular plasma membrane extensions originating from the NB/GMC interface appear within ~ 5 min following closure of the cytokinesis ring (00h04m–00h09m), persist throughout interphase (00h55m) and disappear upon cell rounding at the onset of mitosis (01h03m). c Interphase NB expressing Sep2::GFP (green) and the membrane marker PH::RFP (magenta). d Interphase NBs expressing GFP or YFP-fusions to the indicated proteins localizing to similar tubular extensions at the NB/last-born GMC interface. Cindr::GFP also localizes to centrosomes (arrowhead). e Schematic illustrating the specific characteristics of the NB/last-born GMC interface and the inheritance of this interface by the GMC at each division. Green: midbody. Asterisk: GMCs, same colour code as Fig. 3a. Scale bar in all panels: 5 µm

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