Fig. 3 | Nature Communications

Fig. 3

From: The last-born daughter cell contributes to division orientation of Drosophila larval neuroblasts

Fig. 3

Last-born GMC ablation displaces NB polarization. a Two successive divisions of NBs expressing the membrane marker PH::RFP (magenta), the apical marker Baz::GFP and the centrosome marker Asl::YFP (green) pre- and post-ablation. The ablation takes place between the two frames separated by a lightning symbol. Blue and green arrows: division axis. Yellow arrows: apical centrosome. Arrowheads: limits of Baz::GFP apical crescents. Blue asterisk: GMC prior to ablation. Data from two independent experiments. Scale bar: 5 µm. b Schematic of the two possibilities explaining misaligned divisions following GMC ablation. Green crescent: apical pole. Grey lines: mitotic spindle. Green dots: centrosomes. c Deviation of the division axis following GMC ablation in control NBs (36 ± 20°, n = 35, also shown in Figs. 2c and 5c, data from ten independent experiments), no ablation in sas4 mutant NBs (33 ± 26°, n = 50, also shown in Supplementary Fig. 1d) and GMC ablation in sas4 mutant NBs (49 ± 28°, n = 22), data from six different experiments

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