Fig. 1 | Nature Communications

Fig. 1

From: The last-born daughter cell contributes to division orientation of Drosophila larval neuroblasts

Fig. 1

NBs align their division axis with GMC position. a Two successive divisions of a larval NB expressing the membrane marker PH::RFP. Top row: no obvious movement of the last-born GMC is observed following the first division; bottom row: last-born GMC movement. Arrows: division axis. Asterisk: last-born GMC. Dashed line: NB/GMC interface at prophase. Scale bar: 5 µm. b Angles measured in the previous panel and quantified in the next panel. Angle α corresponds to the alignment of the second division (green arrow) with the previous one (blue arrow). Angle β corresponds to the alignment of the second division (green arrow) with an axis (magenta arrow) defined by the GMC (blue asterisk)/NB interface (magenta line) at prophase. c Distribution of the angles described in the previous panel: alignment of the second division axis with the previous division axis (angle α, 24 ± 15° (standard deviation), n = 37) and with the GMC (angle β, 14 ± 7°, n = 52) from one experiment In this boxplot and every following one: cross: outlier; grey circle: average; red dots: individual measurements; centre line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range. P values displayed over boxplots were calculated using a non-parametric two-tailed Mann–Whitney U Test in this figure and all subsequent ones

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