Fig. 2 | Nature Communications

Fig. 2

From: Analysis of chromatin accessibility uncovers TEAD1 as a regulator of migration in human glioblastoma

Fig. 2

TEAD is the top selectively enriched motif at GSC-specific open chromatin and TEAD1 is its most highly expressed family member across GBMs a, b Homer de novo motif discovery outlines the 20 most highly enriched TF motifs at chromatin accessibility regions defined by the GSC tumor-specific (a) and developmentally shared (b) differential ATAC-seq peak analyses (motifs in bold show selective enrichment in only one peak set). The TEAD motif (with highest scores for TEAD4 and TEAD1) is the top, selectively enriched motif within differential GSC tumor-specific peaks (in red). See also Supplementary Data 1. c Bar graph of rld-normalized gene expression values for all significantly and uniquely enriched GSC tumor-specific TF motifs, generated from parallel RNA-seq data in E+GSC and E−GBM populations. TEAD1 is the only highly expressed gene (top 25th percentile), which is differentially overexpressed in E+GSCs (*p = 0.0496 for TEAD1, **p = 0.006 for ZNF416, n = 3. Bars represent mean ± SEM). d Violin plot of TEAD1-4 expression in TCGA GBM RNA-seqV2 data (n = 150) shows that TEAD1 is the most highly expressed TEAD family member, followed by TEAD2, TEAD3, and TEAD4. Expression shown is log2(normalized counts + 1), normalized as detailed in methods. p-Values from one-sided Wilcoxon matched pairs test. Bar represents median value. e Bar graph of rld-normalized gene expression values for TEAD1-4 derived from RNA-seq E + GSC data (***p < 0.001; n = 3. Bars represent mean ± SEM)

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