Fig. 3 | Nature Communications

Fig. 3

From: Preventing acute asthmatic symptoms by targeting a neuronal mechanism involving carotid body lysophosphatidic acid receptors

Fig. 3

In vivo carotid body-mediated bronchoconstriction. ac The anesthetized in vivo preparation used to measure airway mechanics in carotid sinus nerve intact (solid, n = 6) or denervated (crossed, n = 6) preparations, in response to jugular vein injection of lysophosphatidic acid (LPA 5 µM, blue) or sodium cyanide (NaCN, orange; an independent test of carotid body function). Baseline lung resistance (RL) prior to LPA or NaCN injection: intact = 0.31 ± 0.01 cmH2O s ml−1; denervated = 0.28 ± 0.02 cmH2O s ml−1 (independent t-test: t10 = 1.668, p = 0.126). Baseline lung elastance (EL) prior to LPA or NaCN injection: intact = 6.8 ± 0.3 cmH2Oml−1; denervated = 7.6 ± 0.4 cmH2O ml−1 (independent t-test: t10 = 1.433, p = 0.182). b RL in denervated compared to intact CB preparations—LPA-independent t-test: t10 = 4.933, ***p < 0.001; NaCN-independent t-test: t10 = 2.711, *p = 0.022. c EL, in denervated compared to intact CB preparations—LPA-independent t-test: t10 = 6.772, ***p < 0.00001; NaCN-independent t-test: t10 = 4.202, **p < 0.01. In b and c, RL and EL are normalized to saline (baseline) measurements and presented as mean ± sem

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