Fig. 1 | Nature Communications

Fig. 1

From: Lineage dynamics of murine pancreatic development at single-cell resolution

Fig. 1

Single-cell sequencing identifies broad patterns of cellular heterogeneity in E14.5 murine pancreas. a Overview of murine pancreatic development. b Schematic of experimental approach. c t-Distributed stochastic neighbor embedding (t-SNE) visualization of populations from pooled E14.5 mouse pancreata (n = 14). Each dot represents the transcriptome of a single cell, color-coded according to its cellular identity (epithelial, mesenchymal, or immune/vascular). Each cell compartment contains multiple subpopulations, represented by varying degrees of color shading. d Established marker genes identify epithelial cells (Cdh1+), endocrine cells (Chga+), mesenchymal cells (Vim+ and Col3a1+), endothelial cells (Pecam1+), and immune cells (Rac2+). e Heatmap depicting greater than two-fold differentially expressed genes in each cluster compared to all other clusters. Cells are represented in columns, and genes in rows. Specific genes used to annotate clusters are indicated to the right of the heatmap

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