Fig. 3 | Nature Communications

Fig. 3

From: Antagonistic chemical coupling in self-reconfigurable host–guest protocells

Fig. 3

Disassembly and transformation in self-reconfigurable host–guest protocells. ad Time series of optical microscopy images showing enzyme-mediated disassembly and transformation of an individual GOx-containing proteinosome/myristic acid micelle coacervate droplet in the presence of glucose (10 mM; time t0). Arrow shows location of the single guest proteinosome (low-optical contrast) that remains intact as the host micelle coacervate droplet (high optical contrast) is progressively disassembled and transformed into a corona of elongated fatty acid vesicles (low-optical contrast). The proteinosome is released as an intact micro-capsule (Supplementary Movie 2); scale bars, 50 μm. el As for ad, but imaged by confocal fluorescence microscopy showing presence of intact FITC-labelled guest proteinosome at time t1 (green ring in eh, dashed line in il) within a disassembling Nile Red-doped micelle coacervate droplet (dashed line in eh, red object in il). Progressive outgrowth of red fluorescent elongated protrusions occurs from the surface of the deconstructing micelle coacervate droplet, along with inward contraction into the proteinosome interior (white arrows) (Supplementary Movie 3); scale bars, 50 μm. Numbers and white line in i refer to line profile and specific locations used to measure changes in red fluorescence (see m, n). m, n Spatial- (m) and time (n)-dependent changes in red fluorescence intensities for the host–guest protocell shown in il. Line profiles are shown in m (white line in i), and intensity values measured at three specific locations (1, 2, 3 in i) are shown in n. Error bars in n represent the standard deviation of the fluorescence intensity. oq High-magnification confocal fluorescence microscopy images showing detachment of spherical fatty acid vesicles at the end of elongated protrusions formed at the droplet/water interface over a period of 4 min (white arrows); scale bars, 10 μm. r, s 2D confocal microscopy image (r) and 3D reconstruction (s) of a single proteinosome containing multiple fatty acid vesicles after enzyme-mediated self-induced transformation of a FITC-labelled proteinosome/Nile Red-doped micelle coacervate nested protocell. t, u As for r, s, but after a more rapid decrease in pH (addition of HCl) showing absence of vesicle sequestration in the reconstructed hybrid protocell; scale bars, 20 μm

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