Table 1 OR2AT4 stimulation by Sandalore® treatment regulates different signaling protein phosphorylation pathways in human HFs ex vivo

From: Olfactory receptor OR2AT4 regulates human hair growth

Proteins regulated under 50 µM Sandalore stimulation Type of regulation Proteins regulated under 500 µM Sandalore stimulation Type of regulation
p38α (T180/Y182) ↑(2,5) p38α (T180/Y182) ↑(1,8)
ERK1/2 (T202/Y204, T185/Y187) ↑(1,9) ERK1/2 (T202/Y204, T185/Y187) ↑(1,8)
EGFR (Y1086) ↑(3,2) EGFR (Y1086) ↑(2,6)
MSK1/2 (S376/S360) ↑(2,1) MSK1/2 (S376/S360) ↑(2,1)
PYK2 (Y402) ↑(4,7) PYK2 (Y402) ↑(2,2)
Hsp60 ↓(0,6)   
JNK 1/2/3 (T183/Y185, T221/Y223) ↑(2,2)   
AMPKα1 (T183) ↑(2,0)   
PLC-γ1 (Y783) ↑(2,4)   
   Fgr (Y421) ↑(1,9)
   PRAS40 (T246) ↑(1,9)
   p53 (S392) ↓(0,5)
   p53 (S46) ↓(0,6)
   p53 (S15) ↓(0,5)
  1. PRAS40 proline-rich AKT1 substrate 4049, S46 phosphorylated p5350, p53 phosphoprotein p53, p38α mitogen-activated protein kinases 14, ERK1/2 extracellular signal-regulated kinases1/2, EGFR epidermal growth factor receptor, MSK1/2 mitogen and stress-activated protein kinase 1/2, PYK2 protein tyrosine kinase 2, Hsp60 heat shock protein 60, JNK1/2/3 c-Jun N-terminal protein kinase 1/2/3, AMPKα1 AMP-activated protein kinase α1, PLCγ phospholipase C γ1, Fgr Feline Gardner-Rasheed proto-oncogene, WNK1 WNK lysine-deficient protein kinase 1 isoform
  2. Protein extraction and phospho-kinase assay was performed in treated and vehicle human microdissected HFs following the manufacturer’s protocol (for Supplementary Information, see Methods section). Summary of kinases upregulated at least (1.8×-fold change of the control) or downregulated by >50% of the control by Sandalore® (50 and 500 µM, respectively)