Table 1 Cryo-EM data collection, refinement, and validation statistics

From: Cryo-EM of full-length α-synuclein reveals fibril polymorphs with a common structural kernel

  Rod polymorph Twister polymorph
  EMD-7618 EMD-7619
  PDB:6CU7 PDB:6CU8
Data collection
 Magnification ×130,000 ×130,000
 Defocus range (μm) 1.5–4 1.5–4
 Voltage (kV) 300 300
 Microscope Titan Krios Titan Krios
 Camera Gatan K2 Summit (GIF) Gatan K2 Summit (GIF)
 Frame exposure time (s) 0.2 0.2
 No. of movie frames 50 50
 Total electron dose (e−2) 80 80
 Pixel size (Å) 1.07 1.07
Reconstruction
 Box size (pixel) 432 432
 Inter-box distance (Å) 36 36
 No. of segments extracted 182,253 182,253
 No. of segments after Class2D 23,830 61,698
 No. of segments after Class3D N/Aa 34,091
 Resolution 3.7 3.7
 Map sharpening B-factor (Å2) 100 100
 Helical rise (Å) 2.40 2.40
 Helical twist (°) 179.53 179.06
Atomic model
 No. of protein residues 60 41
 Ramachandran plot values   
   Most favored (%) 87.9 97.4
   Allowed (%) 10.3 2.6
   Disallowed (%) 1.72 0.0
 Rotamer outliers 0.0 0.0
 RMS deviations   
   Bond lengths (Å) 0.01 0.01
   Bond angles (°) 0.89 0.87
 Clashscore 25.67 22.39
 Map CC (whole unit cell) 0.356 0.375
 Map CC (around atoms) 0.754 0.727
  1. N/A not applicable
  2. Particles from Class2D are used in the refinement