Fig. 5 | Nature Communications

Fig. 5

From: Group I Paks are essential for epithelial- mesenchymal transition in an Apc-driven model of colorectal cancer

Fig. 5

Alternative splicing of CD44 contributes to EMT in PID-expressed cells. a Real-time PCR analysis showed relative expression levels of standard isoform, total and variants of CD44 in PID+ and PID- primary cells. Results were normalized to GAPDH. b Immunoblot analysis and quantification of CD44 isoform in PID+ (4, 7, and 26) and PID- (35, 48, and 56) cells. GAPDH served as the internal control. c Immunoblot analysis showed protein levels and localization of hnRNPM, PCBP1, ESRP1, Snai1 and P-Snai1 in PID+ (4 and 26) and PID- (35 and 56) primary cells. Lamin A/C and Vinculin served as loading control for cytoplasmic (C) and nuclear (N) fraction, respectively. Graphs represented quantification of proteins in nuclear and cytosolic fraction. d Immunoblot analysis and quantification of E-Cadherin, N-Cadherin, Zeb1, ESRP1, and Snai1 in PID- primary cells either transduced with retrovirus bearing empty vector (EV), wild-type Snai1 (WT) or virus expressing mutant Snai1-S246A (SA). GAPDH serve as a loading control. e Immunoblot analysis and quantification of ESRP1, CD44, E-Cadherin and GAPDH in PID+ primary cells either transducted with sh-Scramble or shRNA against ESRP1. f Schematic illustration of invasiness inhibition by PID peptide

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