Fig. 1 | Nature Communications

Fig. 1

From: High-throughput chromatin accessibility profiling at single-cell resolution

Fig. 1

µATAC-seq: a nano-well scATAC-seq implementation on the ICELL8 platform. a µATAC-seq workflow. b Distribution of cell counts per well measured by fluorescence microscopy (Hoechst). c µATAC-seq library complexity for null, mouse, and human targeted wells using two separate polymerases (e2Tak and Q5) for well barcoding and amplification (n = 5000 wells). For each sample, the box denotes the interquartile range centered at the median (red line), while the whiskers span the 5th and 95th percentile range. d Correlation between nano-well chips processed with either a e2Tak (replicate 1) or Q5 polymerase (replicate 2) across all accessible loci. e Inter-well mixing of mouse and human µATAC-seq fragments. f Representative population22 and single-cell ATAC-seq genome tracks for the Gapdh locus. g Signal-to-background (percent reads in peaks) as a function of read depth (n = 792). Only cells lying in the upper right quadrant (marked by dashed lines) are retained for downstream analysis

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