Fig. 5 | Nature Communications

Fig. 5

From: Speed dependent descending control of freezing behavior in Drosophila melanogaster

Fig. 5

Silencing DNp09 neurons disrupts freezing but not running. a DNp09 morphology. DNp09 split-GAL4 driving membrane-bound GFP (green) combined with anti-synaptotagmin-HA staining (purple). In bh, red corresponds to DNp09 > Kir2.1 flies, dark gray to DNp09/ + flies and light gray to Kir2.1/ + flies. In bd, n = 60 flies for each condition. b Proportion of freezing flies. Dashed lines represent stimulus presentations. c Percent time spent freezing during stimulation period. d Average (±s.e.m.) fly speed including only time periods classified as walking. Dashed lines indicate stimulus presentations. e Looming-triggered speed profile (average ± s.e.m.) of walking trials that include a pause (DNp09 > Kir2.1 n = 234, DNp09/ + n = 253, Kir2.1/ + n = 259). f Average ( + s.e.m.) speed during baseline period (n = 60 for all conditions). In gh, n = 50 flies for each group. g Proportion of freezing flies for low speed closed-loop looming stimulation. Dashed line indicates onset of stimulus presentations. h Percent time spent freezing during stimulation period for closed-loop experiment. ** denotes p < 0.01, *** denotes p < 0.001, ns not significant. Box plot elements: center line, median; box limits, upper (75) and lower (25) quartiles; whiskers, 1.5x interquartile range