Fig. 3 | Nature Communications

Fig. 3

From: Speed dependent descending control of freezing behavior in Drosophila melanogaster

Fig. 3

Flies flee to looming stimuli. a Average (±s.e.m.) fly speed including only time periods classified as walking. Dashed lines indicate stimulus presentations (in a, b looming n = 280 flies, control n = 298 flies). b Change in walking speed caused by stimulation (baseline period subtracted from stimulation period). c Example trajectories of walking trials. Black section corresponds to looming window and blue sections 500 ms before and after looming. d, e Distribution of path orientations before (d) and after (e) looming. Bar height indicates counts. Stimulus source (screen) was located at 90°. In df, only looming events where flies were walking before and after the stimulus were included (looming n = 1574 trials, control n = 2881 trials). f Looming-triggered speed profile. Average (±s.e.m.) speed in a 1 s window around looming for all walking trials. Blue numbers represent four stages of the response to looming: 1- pre-looming, 2- pause, 3- run, 4- post-looming. Top, size of looming disk (visual angle). g Looming-triggered speed profile of walking trials from the looming condition separated into responses that included a pause (dark blue, n = 806) and responses that did not (light blue, n = 767). h Change in walking speed caused by stimulus presentation (pre-looming period subtracted from post-looming period). i Fraction of flies performing the described behaviors for each of the 20 looming presentations. *** denotes, p < 0.001, ns not significant. Box plot elements: center line, median; box limits, upper (75) and lower (25) quartiles; whiskers, 1.5× interquartile range